Restriction map for
pFN21AB5724
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AocI
Aor51HI ApaI AscI Asp718I AspI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmFI
BsmI BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoT22I FauI FseI FspI HaeII
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MflI MluI MluNI
MroI MscI MslI MspA1I MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
StuI SwaI Tsp45I Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AciI 1474 GCGG CCGC(-3/-1)
AclI 2173 AACGTT AA^CGTT
AhdI 1070 GACGCTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 394 GTGCAC G^TGCAC
ApaLI 394 GTGCAC G^TGCAC
AspEI 1070 GACGCTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 809 CTCGGG C^[CT]CG[GA]G
AvaII 763 GGACC G^G[AT]CC
BciVI 1325 GGATAC GTATCC(6/5)
BclI 1394 TGATCA T^GATCA
BcnI 1149 CCGGG CC^[GC]GG
BsaBI 1455 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 223 GAGGAG GAGGAG(10/8)
BsgI 476 GTGCAG GTGCAG(16/14)
BsoBI 809 CTCGGG C^[CT]CG[GA]G
Bsp1407I 455 TGTACA T^GTACA
BspHI 1005 TCATGA T^CATGA
BsrGI 455 TGTACA T^GTACA
BstXI 766 CCAATTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 675 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 2153 CCATGG C^C[GA][CT]GG
Eam1105I 1070 GACGCTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 675 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 331 GAATTC G^AATTC
FbaI 1394 TGATCA T^GATCA
HapII 1149 CCGG C^CGG
HincII 2176 GTTGAC GT[CT]^[GA]AC
HindII 2176 GTTGAC GT[CT]^[GA]AC
HindIII 129 AAGCTT A^AGCTT
HpaII 1149 CCGG C^CGG
MamI 1455 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 767 CAATTG C^AATTG
MspI 1149 CCGG C^CGG
MunI 767 CAATTG C^AATTG
NciI 1149 CCGGG CC^[GC]GG
NcoI 2153 CCATGG C^CATGG
NheI 1234 GCTAGC G^CTAGC
NlaIV 1152 GGAACC GG[ACGT]^[ACGT]CC
NspI 785 ACATGC [GA]CATG^[CT]
PacI 1345 TTAATTAA TTAAT^TAA
PleI 2058 GACTC GAGTC(4/5)
Psp1406I 2173 AACGTT AA^CGTT
PstI 1275 CTGCAG CTGCA^G
RcaI 1005 TCATGA T^CATGA
ScaI 246 AGTACT AGT^ACT
SfcI 1275 CTGCAG C^T[GA][CT]AG
SpeI 904 ACTAGT A^CTAGT
SspBI 455 TGTACA T^GTACA
TaqI 1565 TCGA T^CGA
TthHB8I 1565 TCGA T^CGA
XcmI 636 CCATGCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwI 649 GGATC GGATC(4/5)
1588 GGATC
AlwNI 323 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
641 CAGCTACTG
Asp700I 719 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1366 GAAACTGTTC
AspHI 6 GAGCAC G[AT]GC[AT]^C
394 GTGCAC
BbvI 478 GCAGC GCAGC(8/12)
640 GCAGC
BsaJI 48 CCAAGG C^C[ACGT][ACGT]GG
2153 CCATGG
BsiHKAI 6 GAGCAC G[AT]GC[AT]^C
394 GTGCAC
Cfr13I 676 GGGCC G^G[ACGT]CC
763 GGACC
EarI 220 GAAGAG CTCTTC(1/4)
2083 GAAGAG
EcoRV 403 GATATC GAT^ATC
1561 GATATC
EcoT14I 48 CCAAGG C^C[AT][AT]GG
2153 CCATGG
Ksp632I 220 GAAGAG CTCTTC(1/4)
2083 GAAGAG
MaeIII 625 GTAAC ^GT[ACGT]AC
1959 GTTAC
MwoI 1234 GCTAGCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1780 GCCACAAAAGC
Sau96I 676 GGGCC G^G[ACGT]CC
763 GGACC
SfaNI 1231 GATGC GCATC(5/9)
1866 GATGC
SspI 196 AATATT AAT^ATT
533 AATATT
StyI 48 CCAAGG C^C[AT][AT]GG
2153 CCATGG
TseI 478 GCAGC G^C[AT]GC
640 GCAGC
XmnI 719 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1366 GAAACTGTTC
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AfaI AluI ApoI AseI
AsnI BbsI BfaI BmyI BpmI
BpuAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HgaI HinfI HphI ItaI
MaeI MaeII MboI MboII MnlI
MseI MvaI NdeII NlaIII PshBI
RsaI Sau3AI ScrFI TfiI Tru9I
Tsp509I TspRI