Restriction map for
pFN21AE9448
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BciVI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BsgI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeI MaeIII MamI MfeI
MluI MseI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
TfiI Tru9I Tsp45I Tsp509I Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 336 CGCG CG^CG
AccIII 302 TCCGGA T^CCGGA
AvaI 640 CCCGAG C^[CT]CG[GA]G
BbeI 1220 GGCGCC GGCGC^C
BbvCI 1197 CCTCAGC CCTCAGC(-5/-2)
BcgI 429 CGAGTTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 473 TGATCA T^GATCA
BcnI 786 CCGGG CC^[GC]GG
BglI 946 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 634 TACGTG [CT]AC^GT[GA]
BsaWI 302 TCCGGA [AT]^CCGG[AT]
BseAI 302 TCCGGA T^CCGGA
BseRI 907 CTCCTC GAGGAG(10/8)
BsiEI 689 CGGCCG CG[GA][CT]^CG
BsoBI 640 CCCGAG C^[CT]CG[GA]G
BspEI 302 TCCGGA T^CCGGA
BspLU11I 317 ACATGT A^CATGT
BsrI 386 CCAGT ACTGG(1/-1)
BstAPI 855 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 336 CGCG CG^CG
BstYI 1205 GGATCT [GA]^GATC[CT]
DdeI 1198 CTCAG C^T[ACGT]AG
DsaI 182 CCACGG C^C[GA][CT]GG
EagI 689 CGGCCG C^GGCCG
EarI 910 CTCTTC CTCTTC(1/4)
EclXI 689 CGGCCG C^GGCCG
Eco52I 689 CGGCCG C^GGCCG
EcoNI 281 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 522 CCCGC CCCGC(4/6)
FbaI 473 TGATCA T^GATCA
HgaI 544 GCGTC GACGC(5/10)
KasI 1220 GGCGCC G^GCGCC
Ksp632I 910 CTCTTC CTCTTC(1/4)
MflI 1205 GGATCT [GA]^GATC[CT]
MroI 302 TCCGGA T^CCGGA
MslI 246 CACGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 336 CGCG CG^CG
NarI 1220 GGCGCC GG^CGCC
NciI 786 CCGGG CC^[GC]GG
NspI 317 ACATGT [GA]CATG^[CT]
PflMI 77 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SexAI 506 ACCTGGT A^CC[AT]GGT
SfiI 945 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1220 GGCGCC GGC^GCC
StuI 1019 AGGCCT AGG^CCT
Van91I 77 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1205 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 543 GGCGTC G[GA]^CG[CT]C
1220 GGCGCC
AfaI 966 GTAC GT^AC
1068 GTAC
AflIII 317 ACATGT A^C[GA][CT]GT
972 ACACGT
AlwI 1410 GATCC GGATC(4/5)
1205 GGATC
BbsI 1395 GAAGAC GAAGAC(2/6)
376 GTCTTC
BpuAI 1395 GAAGAC GAAGAC(2/6)
376 GTCTTC
BsaHI 543 GGCGTC G[GA]^CG[CT]C
1220 GGCGCC
BspMI 309 ACCTGC ACCTGC(4/8)
1091 ACCTGC
BsrBI 1155 CCGCTC CCGCTC(-3/-3)
1167 CCGCTC
BsrDI 1080 CATTGC GCAATG(2/0)
830 GCAATG
CfoI 629 GCGC GCG^C
1221 GCGC
HaeII 628 AGCGCC [GA]GCGC^[CT]
1220 GGCGCC
HhaI 629 GCGC GCG^C
1221 GCGC
Hin1I 543 GGCGTC G[GA]^CG[CT]C
1220 GGCGCC
HinP1I 629 GCGC G^CGC
1221 GCGC
MaeII 635 ACGT A^CGT
974 ACGT
PpuMI 170 GGGTCCC [GA]G^G[AT]CC[CT]
1421 AGGACCC
PstI 285 CTGCAG CTGCA^G
862 CTGCAG
RsaI 966 GTAC GT^AC
1068 GTAC
SacI 449 GAGCTC GAGCT^C
993 GAGCTC
SfaNI 252 GATGC GCATC(5/9)
104 GCATC
SfcI 285 CTGCAG C^T[GA][CT]AG
862 CTGCAG
TspRI 224 CAGTG CA[GC]TG[ACGT][ACGT]^
403 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaII
BalI BanI BanII BbvI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrFI BssKI
BstF5I BstNI BstXI Cac8I Cfr10I
Cfr13I DpnII DraII EaeI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MboI MboII MluNI MnlI MscI
MspA1I MspI MvaI MwoI NaeI
NdeII NgoMIV NlaIII NlaIV PleI
PvuII Sau3AI Sau96I ScrFI StyI
TseI XcmI