Restriction map for
pFN21AE1838
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MluI MroI
MunI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 109 GTAGAC GT^[AC][GT]AC
AccII 36 CGCG CG^CG
AfaI 778 GTAC GT^AC
Asp700I 864 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 825 CTCGGG C^[CT]CG[GA]G
AvaII 517 GGACC G^G[AT]CC
BalI 858 TGGCCA TGG^CCA
BbvI 1017 GCTGC GCAGC(8/12)
BciVI 789 GGATAC GTATCC(6/5)
BmyI 287 GGGCAC G[AGT]GC[ACT]^C
BsmFI 830 GGGAC GGGAC(10/14)
BsoBI 825 CTCGGG C^[CT]CG[GA]G
Bsp1286I 287 GGGCAC G[AGT]GC[ACT]^C
BspHI 723 TCATGA T^CATGA
BsrFI 519 ACCGGC [GA]^CCGG[CT]
BstUI 36 CGCG CG^CG
BstXI 176 CCAATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 264 GGATCT [GA]^GATC[CT]
Cfr10I 519 ACCGGC [GA]^CCGG[CT]
Cfr13I 517 GGACC G^G[ACGT]CC
DraI 427 TTTAAA TTT^AAA
EaeI 858 TGGCCA [CT]^GGCC[GA]
EarI 868 CTCTTC CTCTTC(1/4)
EcoRI 403 GAATTC G^AATTC
HaeII 42 GGCGCT [GA]GCGC^[CT]
HapII 520 CCGG C^CGG
HpaII 520 CCGG C^CGG
HphI 682 TCACC GGTGA(8/7)
Ksp632I 868 CTCTTC CTCTTC(1/4)
MaeIII 946 GTGAC ^GT[ACGT]AC
MflI 264 GGATCT [GA]^GATC[CT]
MluNI 858 TGGCCA TGG^CCA
MscI 858 TGGCCA TGG^CCA
MspA1I 509 CAGCTG C[AC]G^C[GT]G
MspI 520 CCGG C^CGG
MvnI 36 CGCG CG^CG
PleI 218 GACTC GAGTC(4/5)
PvuII 509 CAGCTG CAG^CTG
RcaI 723 TCATGA T^CATGA
RsaI 778 GTAC GT^AC
SapI 867 GCTCTTC GCTCTTC(1/4)
Sau96I 517 GGACC G^G[ACGT]CC
SmlI 781 CTTGAG C^T[CT][GA]AG
SspI 676 AATATT AAT^ATT
TaqI 78 TCGA T^CGA
TseI 1017 GCTGC G^C[AT]GC
Tsp45I 946 GTGAC ^GT[GC]AC
TthHB8I 78 TCGA T^CGA
XhoII 264 GGATCT [GA]^GATC[CT]
XmnI 864 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AciI 912 CCGC CCGC(-3/-1)
6 GCGG
BpmI 90 CTCCAG CTGGAG(16/14)
245 CTGGAG
BsiYI 820 CCTTCCTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
962 CCAACAATGGG
BslI 820 CCTTCCTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
962 CCAACAATGGG
BsrI 599 ACTGG ACTGG(1/-1)
795 ACTGG
BstF5I 850 CATCC GGATG(2/0)
915 CATCC
CfoI 37 GCGC GCG^C
43 GCGC
EcoT14I 283 CCAAGG C^C[AT][AT]GG
981 CCAAGG
Fnu4HI 6 GCGGC GC^[ACGT]GC
1017 GCTGC
FokI 850 CATCC GGATG(9/13)
915 CATCC
HaeIII 324 GGCC GG^CC
859 GGCC
HhaI 37 GCGC GCG^C
43 GCGC
HinP1I 37 GCGC G^CGC
43 GCGC
ItaI 6 GCGGC GC^[ACGT]GC
1017 GCTGC
StyI 283 CCAAGG C^C[AT][AT]GG
981 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AcsI AluI AlwI ApoI BsaJI
BssKI BstNI Cac8I DdeI DpnII
EcoRII HinfI MboI MboII MnlI
MseI MslI MvaI NdeII NlaIII
Sau3AI ScrFI SfaNI SfcI TfiI
Tru9I Tsp509I TspRI