Restriction map for
pFN21AB5755
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AocI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BclI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I ClaI DraI
DraIII EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I FbaI FseI FspI
HindIII HpaI KasI KpnI KspI
MaeII MamI MflI MluI MluNI
MroI MscI MseI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI SacI SacII SalI
SapI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI SwaI Tru9I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 850 CGCG CG^CG
AcyI 1497 GACGCC G[GA]^CG[CT]C
AlwNI 820 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 755 AGCGCT AGC^GCT
Asp700I 13 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 2226 CGAACTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 875 CCGGG CC^[GC]GG
BlpI 2010 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2010 GCTAAGC GC^T[ACGT]AGC
BsaHI 1497 GACGCC G[GA]^CG[CT]C
BsaWI 2084 ACCGGA [AT]^CCGG[AT]
BsrDI 559 CATTGC GCAATG(2/0)
BsrFI 1220 ACCGGC [GA]^CCGG[CT]
BstUI 850 CGCG CG^CG
CelII 2010 GCTAAGC GC^T[ACGT]AGC
CfoI 756 GCGC GCG^C
Cfr10I 1220 ACCGGC [GA]^CCGG[CT]
CpoI 2086 CGGACCG CG^G[AT]CCG
DrdI 1321 GACCCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2246 TGGCCG [CT]^GGCC[GA]
Eco47III 755 AGCGCT AGC^GCT
EcoNI 2142 CCTTCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 172 GAATTC G^AATTC
EcoRV 1833 GATATC GAT^ATC
EcoT22I 556 ATGCAT ATGCA^T
HaeII 755 AGCGCT [GA]GCGC^[CT]
HhaI 756 GCGC GCG^C
Hin1I 1497 GACGCC G[GA]^CG[CT]C
HinP1I 756 GCGC G^CGC
HincII 1851 GTTGAC GT[CT]^[GA]AC
HindII 1851 GTTGAC GT[CT]^[GA]AC
MfeI 1081 CAATTG C^AATTG
MunI 1081 CAATTG C^AATTG
MvnI 850 CGCG CG^CG
NciI 875 CCGGG CC^[GC]GG
NsiI 556 ATGCAT ATGCA^T
NspI 1941 GCATGT [GA]CATG^[CT]
PpuMI 2283 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 118 CAGCTG CAG^CTG
RsrII 2086 CGGACCG CG^G[AT]CCG
SbfI 179 CCTGCAGG CCTGCA^GG
Sse8387I 179 CCTGCAGG CCTGCA^GG
XmnI 13 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AlwI 472 GATCC GGATC(4/5)
502 GATCC
ApaI 481 GGGCCC GGGCC^C
992 GGGCCC
AspI 339 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
597 GACCATGTC
BbsI 1230 GAAGAC GAAGAC(2/6)
1435 GAAGAC
BfaI 404 CTAG C^TAG
1964 CTAG
BpuAI 1230 GAAGAC GAAGAC(2/6)
1435 GAAGAC
BsgI 783 CTGCAC GTGCAG(16/14)
146 GTGCAG
BsmAI 1420 GAGAC GTCTC(1/5)
1555 GAGAC
BspMI 178 ACCTGC ACCTGC(4/8)
414 ACCTGC
BstXI 1134 CCATGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1765 CCACATTCCTGG
DpnII 472 GATC ^GATC
502 GATC
EarI 544 GAAGAG CTCTTC(1/4)
2210 GAAGAG
HgaI 1497 GACGC GACGC(5/10)
1328 GCGTC
Ksp632I 544 GAAGAG CTCTTC(1/4)
2210 GAAGAG
MaeI 404 CTAG C^TAG
1964 CTAG
MboI 472 GATC ^GATC
502 GATC
MspA1I 118 CAGCTG C[AC]G^C[GT]G
780 CCGCTG
NcoI 997 CCATGG C^CATGG
1134 CCATGG
NdeII 472 GATC ^GATC
502 GATC
PflFI 339 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
597 GACCATGTC
Sau3AI 472 GATC ^GATC
502 GATC
ScaI 1034 AGTACT AGT^ACT
1340 AGTACT
SfaNI 1288 GATGC GCATC(5/9)
362 GCATC
StuI 620 AGGCCT AGG^CCT
1885 AGGCCT
TaqI 1934 TCGA T^CGA
2300 TCGA
Tth111I 339 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
597 GACCATGTC
TthHB8I 1934 TCGA T^CGA
2300 TCGA
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
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AciI AcsI AfaI AluI Alw44I
ApaLI ApoI AspHI AvaII BanI
BanII BbvI BciVI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI BsmI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII DsaI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboII MnlI MslI MspI MvaI
MwoI NlaIII NlaIV PflMI PleI
PstI RsaI Sau96I ScrFI SfcI
SmlI StyI TfiI TseI Tsp45I
Tsp509I TspRI Van91I XcmI