Restriction map for
pFN21AE6604
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaJI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DraII DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FokI
FseI FspI HaeII HaeIII HgaI
HhaI Hin1I HinP1I HindIII KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI TspRI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 722 AAATTT [GA]^AATT[CT]
AgeI 12 ACCGGT A^CCGGT
Alw44I 231 GTGCAC G^TGCAC
ApaLI 231 GTGCAC G^TGCAC
ApoI 722 AAATTT [GA]^AATT[CT]
AspHI 231 GTGCAC G[AT]GC[AT]^C
BamHI 66 GGATCC G^GATCC
BbsI 321 GTCTTC GAAGAC(2/6)
BbvI 36 GCTGC GCAGC(8/12)
BfaI 355 CTAG C^TAG
BglII 624 AGATCT A^GATCT
BmyI 231 GTGCAC G[AGT]GC[ACT]^C
BpuAI 321 GTCTTC GAAGAC(2/6)
BsaWI 12 ACCGGT [AT]^CCGG[AT]
BsiHKAI 231 GTGCAC G[AT]GC[AT]^C
BsmAI 151 GTCTC GTCTC(1/5)
BsmI 207 GAATGC GAATGC(1/-1)
Bsp1286I 231 GTGCAC G[AGT]GC[ACT]^C
Cac8I 661 GCTGGC GC[ACGT]^[ACGT]GC
DraIII 560 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 377 GACCAAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 52 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 70 CCCGC CCCGC(4/6)
Fnu4HI 36 GCTGC GC^[ACGT]GC
HincII 87 GTTAAC GT[CT]^[GA]AC
HindII 87 GTTAAC GT[CT]^[GA]AC
HpaI 87 GTTAAC GTT^AAC
HphI 283 GGTGA GGTGA(8/7)
ItaI 36 GCTGC GC^[ACGT]GC
MaeI 355 CTAG C^TAG
MseI 88 TTAA T^TAA
MspA1I 659 CAGCTG C[AC]G^C[GT]G
MwoI 73 GCTTTTACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 66 GGATCC GG[ACGT]^[ACGT]CC
NspI 170 ACATGC [GA]CATG^[CT]
PinAI 12 ACCGGT A^CCGGT
PleI 384 GAGTC GAGTC(4/5)
PstI 53 CTGCAG CTGCA^G
PvuII 659 CAGCTG CAG^CTG
SexAI 437 ACCAGGT A^CC[AT]GGT
SfcI 53 CTGCAG C^T[GA][CT]AG
SmlI 682 CTCAAG C^T[CT][GA]AG
SspI 503 AATATT AAT^ATT
TaqI 430 TCGA T^CGA
Tru9I 88 TTAA T^TAA
TseI 36 GCTGC G^C[AT]GC
TthHB8I 430 TCGA T^CGA
XcmI 438 CCAGGTAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 71 CCGC CCGC(-3/-1)
261 CCGC
AvaII 258 GGACC G^G[AT]CC
376 GGACC
BsmFI 386 GTCCC GGGAC(10/14)
464 GTCCC
BspMI 51 ACCTGC ACCTGC(4/8)
159 ACCTGC
BsrFI 12 ACCGGT [GA]^CCGG[CT]
568 GCCGGT
BssKI 317 CCTGG ^CC[ACGT]GG
438 CCAGG
BstNI 317 CCTGG CC^[AT]GG
438 CCAGG
Cfr10I 12 ACCGGT [GA]^CCGG[CT]
568 GCCGGT
Cfr13I 258 GGACC G^G[ACGT]CC
376 GGACC
EcoRII 317 CCTGG ^CC[AT]GG
438 CCAGG
HapII 13 CCGG C^CGG
569 CCGG
HpaII 13 CCGG C^CGG
569 CCGG
MboII 651 GAAGA GAAGA(8/7)
322 TCTTC
MnlI 196 GAGG CCTC(7/6)
256 GAGG
MspI 13 CCGG C^CGG
569 CCGG
MvaI 317 CCTGG CC^[AT]GG
438 CCAGG
NlaIII 171 CATG CATG^
673 CATG
Sau96I 258 GGACC G^G[ACGT]CC
376 GGACC
ScrFI 317 CCTGG CC^[ACGT]GG
438 CCAGG
TfiI 288 GAATC G^A[AT]TC
643 GATTC
Tsp45I 305 GTGAC ^GT[GC]AC
587 GTGAC
Tsp509I 453 AATT ^AATT
723 AATT
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# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AfaI AluI AlwI BsiYI BslI
BsrI BstYI DdeI DpnII HinfI
MaeIII MboI MflI NdeII RsaI
Sau3AI XhoII