Restriction map for
pFN21AE3016
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# NO CUT ENZYMES ( 196 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MaeIII MamI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI Tru9I Tsp45I
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 187 GTAGAC GT^[AC][GT]AC
AciI 196 GCGG CCGC(-3/-1)
AfaI 288 GTAC GT^AC
AvaII 181 GGTCC G^G[AT]CC
BanII 199 GAGCCC G[GA]GC[CT]^C
BciVI 4 GTATCC GTATCC(6/5)
BclI 227 TGATCA T^GATCA
BcnI 291 CCCGG CC^[GC]GG
BglI 49 GCCATGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 199 GAGCCC G[AGT]GC[ACT]^C
BsaJI 299 CCCTGG C^C[ACGT][ACGT]GG
BseRI 242 CTCCTC GAGGAG(10/8)
BsiYI 299 CCCTGGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 299 CCCTGGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 79 GTCCC GGGAC(10/14)
Bsp1286I 199 GAGCCC G[AGT]GC[ACT]^C
BstNI 300 CCTGG CC^[AT]GG
BstXI 272 CCAGACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 181 GGTCC G^G[ACGT]CC
DpnII 228 GATC ^GATC
DraII 180 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 293 CGGCCA [CT]^GGCC[GA]
EcoO109I 180 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRII 300 CCTGG ^CC[AT]GG
FbaI 227 TGATCA T^GATCA
HapII 292 CCGG C^CGG
HpaII 292 CCGG C^CGG
HphI 89 TCACC GGTGA(8/7)
MboI 228 GATC ^GATC
MspI 292 CCGG C^CGG
MvaI 300 CCTGG CC^[AT]GG
NciI 291 CCCGG CC^[GC]GG
NdeII 228 GATC ^GATC
NlaIV 198 GGAGCC GG[ACGT]^[ACGT]CC
PpuMI 180 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 288 GTAC GT^AC
Sau3AI 228 GATC ^GATC
Sau96I 181 GGTCC G^G[ACGT]CC
SfcI 185 CTGTAG C^T[GA][CT]AG
Tsp509I 252 AATT ^AATT
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# TWO CUT ENZYMES ( 12 enzymes) #
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BpmI 103 CTCCAG CTGGAG(16/14)
270 CTCCAG
BssKI 291 CCCGG ^CC[ACGT]GG
300 CCTGG
BstF5I 110 GGATG GGATG(2/0)
263 GGATG
DdeI 151 CTGAG C^T[ACGT]AG
313 CTGAG
FokI 110 GGATG GGATG(9/13)
263 GGATG
HaeIII 57 GGCC GG^CC
294 GGCC
MspA1I 44 CAGCTG C[AC]G^C[GT]G
277 CAGCTG
NspI 39 GCATGC [GA]CATG^[CT]
333 GCATGC
PvuII 44 CAGCTG CAG^CTG
277 CAGCTG
ScrFI 291 CCCGG CC^[ACGT]GG
300 CCTGG
SfaNI 111 GATGC GCATC(5/9)
321 GATGC
SphI 39 GCATGC GCATG^C
333 GCATGC
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
AluI BbvI Cac8I Fnu4HI ItaI
MboII MnlI MwoI NlaIII TseI