Restriction map for
pFN21AE2207
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsiEI
BsiWI BsmAI BsmBI Bsp1407I BspDI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MscI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI Tth111I TthHB8I XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 461 TCCGGA T^CCGGA
AfaI 301 GTAC GT^AC
ApaI 321 GGGCCC GGGCC^C
AseI 247 ATTAAT AT^TAAT
AsnI 247 ATTAAT AT^TAAT
AspHI 268 GAGCAC G[AT]GC[AT]^C
AvaI 935 CCCGGG C^[CT]CG[GA]G
BanII 321 GGGCCC G[GA]GC[CT]^C
BfaI 764 CTAG C^TAG
BpmI 919 CTGGAG CTGGAG(16/14)
BsaWI 461 TCCGGA [AT]^CCGG[AT]
BseAI 461 TCCGGA T^CCGGA
BseRI 191 CTCCTC GAGGAG(10/8)
BsiHKAI 268 GAGCAC G[AT]GC[AT]^C
BsmI 571 GAATGC GAATGC(1/-1)
BsoBI 935 CCCGGG C^[CT]CG[GA]G
BspEI 461 TCCGGA T^CCGGA
BspHI 231 TCATGA T^CATGA
BsrDI 149 GCAATG GCAATG(2/0)
BsrFI 614 ACCGGC [GA]^CCGG[CT]
BstEII 413 GGTCACC G^GT[ACGT]ACC
BstPI 413 GGTCACC G^GT[ACGT]ACC
Cac8I 361 GCCAGC GC[ACGT]^[ACGT]GC
Cfr10I 614 ACCGGC [GA]^CCGG[CT]
EarI 387 CTCTTC CTCTTC(1/4)
EcoNI 823 CCTGCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 413 GGTCACC G^GT[ACGT]ACC
Ksp632I 387 CTCTTC CTCTTC(1/4)
MaeI 764 CTAG C^TAG
MroI 461 TCCGGA T^CCGGA
MseI 248 TTAA T^TAA
MslI 19 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 430 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 228 GACTC GAGTC(4/5)
PshBI 247 ATTAAT AT^TAAT
RcaI 231 TCATGA T^CATGA
RsaI 301 GTAC GT^AC
ScaI 300 AGTACT AGT^ACT
SmaI 935 CCCGGG CCC^GGG
Tru9I 248 TTAA T^TAA
Tsp45I 414 GTCAC ^GT[GC]AC
Tsp509I 246 AATT ^AATT
Van91I 430 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 763 TCTAGA T^CTAGA
XcmI 9 CCAAAATCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 935 CCCGGG C^CCGGG
XmaI 935 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwNI 397 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
431 CAGTCCCTG
BamHI 3 GGATCC G^GATCC
866 GGATCC
BcnI 935 CCCGG CC^[GC]GG
936 CCGGG
BmyI 268 GAGCAC G[AGT]GC[ACT]^C
321 GGGCCC
Bsp1286I 268 GAGCAC G[AGT]GC[ACT]^C
321 GGGCCC
BspMI 186 ACCTGC ACCTGC(4/8)
658 ACCTGC
BstYI 3 GGATCC [GA]^GATC[CT]
866 GGATCC
HphI 177 GGTGA GGTGA(8/7)
415 TCACC
MaeIII 414 GTCAC ^GT[ACGT]AC
611 GTAAC
MboII 388 TCTTC GAAGA(8/7)
683 TCTTC
MflI 3 GGATCC [GA]^GATC[CT]
866 GGATCC
MwoI 365 GCTCTGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
875 GCCCAGGGTGC
NciI 935 CCCGG CC^[GC]GG
936 CCGGG
NspI 294 ACATGC [GA]CATG^[CT]
662 GCATGT
PpuMI 515 AGGACCT [GA]G^G[AT]CC[CT]
586 AGGACCC
SfaNI 833 GATGC GCATC(5/9)
680 GCATC
TfiI 96 GATTC G^A[AT]TC
715 GATTC
XhoII 3 GGATCC [GA]^GATC[CT]
866 GGATCC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AluI AlwI AvaII BbvI BsaJI
BsgI BsiYI BslI BsmFI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MboI MnlI MspI MvaI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI TseI TspRI