Restriction map for
pFN21AE3017
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AviII AvrII BamHI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BlnI BmyI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I MaeI MaeII MamI MfeI
MluI MroI MseI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI SexAI SfiI SfoI SfuI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI TfiI Tru9I TthHB8I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 318 GAATTT [GA]^AATT[CT]
AfaI 150 GTAC GT^AC
ApoI 318 GAATTT [GA]^AATT[CT]
AspI 89 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 293 CCCGAG C^[CT]CG[GA]G
AvaII 81 GGACC G^G[AT]CC
BalI 143 TGGCCA TGG^CCA
BanI 288 GGCACC G^G[CT][GA]CC
BbsI 330 GAAGAC GAAGAC(2/6)
BcnI 279 CCCGG CC^[GC]GG
BlpI 229 GCTGAGC GC^T[ACGT]AGC
BpmI 16 CTCCAG CTGGAG(16/14)
Bpu1102I 229 GCTGAGC GC^T[ACGT]AGC
BpuAI 330 GAAGAC GAAGAC(2/6)
BsoBI 293 CCCGAG C^[CT]CG[GA]G
BsrFI 67 ACCGGC [GA]^CCGG[CT]
BssHII 23 GCGCGC G^CGCGC
BstXI 41 CCATCGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 229 GCTGAGC GC^T[ACGT]AGC
Cfr10I 67 ACCGGC [GA]^CCGG[CT]
DdeI 230 CTGAG C^T[ACGT]AG
DsaI 125 CCGCGG C^C[GA][CT]GG
EaeI 143 TGGCCA [CT]^GGCC[GA]
FauI 127 GCGGG CCCGC(4/6)
HaeII 70 GGCGCT [GA]GCGC^[CT]
HinfI 89 GACTC G^A[ACGT]TC
HphI 65 TCACC GGTGA(8/7)
KspI 125 CCGCGG CCGC^GG
MaeIII 64 GTCAC ^GT[ACGT]AC
MluNI 143 TGGCCA TGG^CCA
MscI 143 TGGCCA TGG^CCA
MspA1I 125 CCGCGG C[AC]G^C[GT]G
NciI 279 CCCGG CC^[GC]GG
PflFI 89 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 89 GACTC GAGTC(4/5)
RsaI 150 GTAC GT^AC
SacII 125 CCGCGG CCGC^GG
ScaI 149 AGTACT AGT^ACT
SfaNI 196 GATGC GCATC(5/9)
SgrAI 66 CACCGGCG C[GA]^CCGG[CT]G
SmlI 250 CTTGAG C^T[CT][GA]AG
Tsp45I 64 GTCAC ^GT[GC]AC
Tsp509I 319 AATT ^AATT
TspRI 19 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 89 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 146 CCAAGTACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 24 CGCG CG^CG
126 CGCG
AlwNI 97 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
136 CAGGAACTG
Asp700I 318 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
330 GAAGACTTTC
BglII 213 AGATCT A^GATCT
262 AGATCT
BsiYI 48 CCTGGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
279 CCCGGCTATGG
BslI 48 CCTGGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
279 CCCGGCTATGG
BsmFI 111 GTCCC GGGAC(10/14)
219 GTCCC
BsrI 141 ACTGG ACTGG(1/-1)
18 CCAGT
BstF5I 341 CATCC GGATG(2/0)
237 GGATG
BstUI 24 CGCG CG^CG
126 CGCG
BstYI 213 AGATCT [GA]^GATC[CT]
262 AGATCT
Cfr13I 51 GGCCC G^G[ACGT]CC
81 GGACC
DpnII 214 GATC ^GATC
263 GATC
FokI 341 CATCC GGATG(9/13)
237 GGATG
HaeIII 51 GGCC GG^CC
144 GGCC
HapII 68 CCGG C^CGG
280 CCGG
HpaII 68 CCGG C^CGG
280 CCGG
MboI 214 GATC ^GATC
263 GATC
MflI 213 AGATCT [GA]^GATC[CT]
262 AGATCT
MspI 68 CCGG C^CGG
280 CCGG
MvnI 24 CGCG CG^CG
126 CGCG
NdeII 214 GATC ^GATC
263 GATC
PstI 103 CTGCAG CTGCA^G
256 CTGCAG
Sau3AI 214 GATC ^GATC
263 GATC
Sau96I 51 GGCCC G^G[ACGT]CC
81 GGACC
SfcI 103 CTGCAG C^T[GA][CT]AG
256 CTGCAG
XhoII 213 AGATCT [GA]^GATC[CT]
262 AGATCT
XmnI 318 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
330 GAAGACTTTC
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI AluI BbvI BsaJI BssKI
BstNI Cac8I CfoI EcoRII Fnu4HI
HhaI HinP1I ItaI MboII MnlI
MvaI MwoI NlaIV ScrFI TseI