Restriction map for
pFN21AE5547
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII CfoI ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 994 GTAGAC GT^[AC][GT]AC
AciI 1137 GCGG CCGC(-3/-1)
AcsI 446 GAATTT [GA]^AATT[CT]
AflIII 831 ACGTGT A^C[GA][CT]GT
AlwNI 88 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 636 CCTCAGG CC^T[ACGT]AGG
ApoI 446 GAATTT [GA]^AATT[CT]
AvaI 56 CCCGGG C^[CT]CG[GA]G
BanI 1176 GGCACC G^G[CT][GA]CC
BfaI 713 CTAG C^TAG
BpmI 1141 CTGGAG CTGGAG(16/14)
BseRI 298 GAGGAG GAGGAG(10/8)
BsgI 63 GTGCAG GTGCAG(16/14)
BsmBI 1186 GAGACG CGTCTC(1/5)
BsoBI 56 CCCGGG C^[CT]CG[GA]G
BspMI 1179 ACCTGC ACCTGC(4/8)
BsrFI 748 ACCGGC [GA]^CCGG[CT]
BssSI 620 CACGAG CACGAG(-5/-1)
BstF5I 381 GGATG GGATG(2/0)
Bsu36I 636 CCTCAGG CC^T[ACGT]AGG
Cfr10I 748 ACCGGC [GA]^CCGG[CT]
DraII 662 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 977 CCATGG C^C[GA][CT]GG
EarI 82 CTCTTC CTCTTC(1/4)
Eco81I 636 CCTCAGG CC^T[ACGT]AGG
EcoO109I 662 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 7 GATATC GAT^ATC
EcoT22I 688 ATGCAT ATGCA^T
FokI 381 GGATG GGATG(9/13)
Ksp632I 82 CTCTTC CTCTTC(1/4)
MaeI 713 CTAG C^TAG
NcoI 977 CCATGG C^CATGG
NsiI 688 ATGCAT ATGCA^T
NspI 686 ACATGC [GA]CATG^[CT]
PflMI 1167 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1181 CTGCAG CTGCA^G
SfaNI 349 GCATC GCATC(5/9)
SmaI 56 CCCGGG CCC^GGG
SmlI 279 CTTGAG C^T[CT][GA]AG
SspI 223 AATATT AAT^ATT
StuI 897 AGGCCT AGG^CCT
TaqI 98 TCGA T^CGA
TthHB8I 98 TCGA T^CGA
Van91I 1167 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 712 TCTAGA T^CTAGA
XcmI 1130 CCAGGAAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 56 CCCGGG C^CCGGG
XmaI 56 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BbrPI 830 CACGTG CAC^GTG
1060 CACGTG
BsaAI 830 CACGTG [CT]AC^GT[GA]
1060 CACGTG
BsmFI 679 GGGAC GGGAC(10/14)
944 GGGAC
BsrDI 51 CATTGC GCAATG(2/0)
1116 CATTGC
BstYI 624 AGATCC [GA]^GATC[CT]
1126 AGATCC
Cac8I 61 GCGTGC GC[ACGT]^[ACGT]GC
153 GCTGGC
EcoT14I 894 CCAAGG C^C[AT][AT]GG
977 CCATGG
HgaI 150 GACGC GACGC(5/10)
1188 GACGC
HincII 139 GTTGAC GT[CT]^[GA]AC
904 GTTGAC
HindII 139 GTTGAC GT[CT]^[GA]AC
904 GTTGAC
HphI 545 TCACC GGTGA(8/7)
704 TCACC
MaeII 831 ACGT A^CGT
1061 ACGT
MflI 624 AGATCC [GA]^GATC[CT]
1126 AGATCC
MseI 503 TTAA T^TAA
553 TTAA
MspA1I 88 CAGCTG C[AC]G^C[GT]G
860 CAGCTG
PmaCI 830 CACGTG CAC^GTG
1060 CACGTG
PmlI 830 CACGTG CAC^GTG
1060 CACGTG
PvuII 88 CAGCTG CAG^CTG
860 CAGCTG
SfcI 697 CTACAG C^T[GA][CT]AG
1181 CTGCAG
StyI 894 CCAAGG C^C[AT][AT]GG
977 CCATGG
Tru9I 503 TTAA T^TAA
553 TTAA
XhoII 624 AGATCC [GA]^GATC[CT]
1126 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AfaI AluI AlwI AvaII BbvI
BcnI BsaJI BsiYI BslI BsmAI
BsmI BsrI BssKI BstNI Cfr13I
DdeI DpnII EcoRII Fnu4HI HaeIII
HapII HinfI HpaII ItaI MaeIII
MboI MboII MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI TfiI TseI Tsp45I Tsp509I
TspRI