Restriction map for
pFN21AE2205
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsoBI BspDI BspEI BsrDI
BsrFI BssHII BssSI Bst1107I BstBI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoT22I FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI KspI
MamI MluI MroI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 487 GTAGAC GT^[AC][GT]AC
AflIII 1572 ACATGT A^C[GA][CT]GT
BalI 353 TGGCCA TGG^CCA
BanI 1341 GGTGCC G^G[CT][GA]CC
BbvCI 813 CCTCAGC CCTCAGC(-5/-2)
BcgI 368 GCAGCACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1355 GGATAC GTATCC(6/5)
BclI 1428 TGATCA T^GATCA
BcnI 22 CCGGG CC^[GC]GG
BsmBI 1294 CGTCTC CGTCTC(1/5)
BsmI 848 GCATTC GAATGC(1/-1)
Bsp1407I 380 TGTACA T^GTACA
BspHI 1097 TCATGA T^CATGA
BspLU11I 1572 ACATGT A^CATGT
BspMI 1058 ACCTGC ACCTGC(4/8)
BsrBI 716 CCGCTC CCGCTC(-3/-3)
BsrGI 380 TGTACA T^GTACA
BstAPI 1489 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1288 GGTTACC G^GT[ACGT]ACC
BstPI 1288 GGTTACC G^GT[ACGT]ACC
DraIII 427 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1425 GACTGATCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 353 TGGCCA [CT]^GGCC[GA]
EcoNI 1671 CCTTCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1288 GGTTACC G^GT[ACGT]ACC
EcoRI 1188 GAATTC G^AATTC
EcoRV 1726 GATATC GAT^ATC
FauI 715 CCCGC CCCGC(4/6)
FbaI 1428 TGATCA T^GATCA
HapII 22 CCGG C^CGG
HindIII 645 AAGCTT A^AGCTT
HpaII 22 CCGG C^CGG
MfeI 1582 CAATTG C^AATTG
MluNI 353 TGGCCA TGG^CCA
MscI 353 TGGCCA TGG^CCA
MspI 22 CCGG C^CGG
MunI 1582 CAATTG C^AATTG
NciI 22 CCGGG CC^[GC]GG
NcoI 413 CCATGG C^CATGG
NdeI 1719 CATATG CA^TATG
NspI 1572 ACATGT [GA]CATG^[CT]
PshAI 888 GACCTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1097 TCATGA T^CATGA
SapI 760 GAAGAGC GCTCTTC(1/4)
ScaI 1331 AGTACT AGT^ACT
SmlI 1675 CTCAAG C^T[CT][GA]AG
SspBI 380 TGTACA T^GTACA
SspI 1382 AATATT AAT^ATT
XbaI 870 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 16 enzymes) #
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AciI 716 CCGC CCGC(-3/-1)
971 CCGC
AcsI 796 GAATTT [GA]^AATT[CT]
1188 GAATTC
ApoI 796 GAATTT [GA]^AATT[CT]
1188 GAATTC
BpmI 5 CTCCAG CTGGAG(16/14)
1042 CTCCAG
BseRI 465 CTCCTC GAGGAG(10/8)
1534 GAGGAG
BsgI 1060 CTGCAC GTGCAG(16/14)
1021 GTGCAG
BsmFI 1067 GGGAC GGGAC(10/14)
1604 GGGAC
DsaI 413 CCATGG C^C[GA][CT]GG
1031 CCACGG
EarI 760 GAAGAG CTCTTC(1/4)
1557 GAAGAG
Ksp632I 760 GAAGAG CTCTTC(1/4)
1557 GAAGAG
MaeII 893 ACGT A^CGT
1470 ACGT
PflMI 853 CCAGTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1614 CCAGTTAATGG
PstI 1312 CTGCAG CTGCA^G
1487 CTGCAG
SexAI 471 ACCTGGT A^CC[AT]GGT
1337 ACCAGGT
Van91I 853 CCAGTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1614 CCAGTTAATGG
XcmI 520 CCAGCATTTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1394 CCAAGGAGGATGTGG
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
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AfaI AluI AlwI AlwNI ApaI
Asp700I AvaII BanII BbsI BbvI
BfaI BmyI BpuAI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI