Restriction map for
pFN21AE4054
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# NO CUT ENZYMES ( 186 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I FauI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MamI MflI
MluI MluNI MroI MscI MspA1I
MspI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AccII 453 CGCG CG^CG
AciI 454 GCGG CCGC(-3/-1)
AcsI 485 AAATTT [GA]^AATT[CT]
AflIII 880 ACATGT A^C[GA][CT]GT
ApoI 485 AAATTT [GA]^AATT[CT]
BbsI 763 GAAGAC GAAGAC(2/6)
BclI 380 TGATCA T^GATCA
BfaI 644 CTAG C^TAG
BmyI 649 GTGCCC G[AGT]GC[ACT]^C
BpmI 630 CTGGAG CTGGAG(16/14)
BpuAI 763 GAAGAC GAAGAC(2/6)
BseRI 197 CTCCTC GAGGAG(10/8)
BsiYI 432 CCAGGGGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 432 CCAGGGGACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 649 GTGCCC G[AGT]GC[ACT]^C
BspLU11I 880 ACATGT A^CATGT
BstUI 453 CGCG CG^CG
EcoT22I 849 ATGCAT ATGCA^T
FbaI 380 TGATCA T^GATCA
MaeI 644 CTAG C^TAG
MfeI 602 CAATTG C^AATTG
MseI 820 TTAA T^TAA
MslI 528 CATTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 602 CAATTG C^AATTG
MvnI 453 CGCG CG^CG
NsiI 849 ATGCAT ATGCA^T
SphI 847 GCATGC GCATG^C
Tru9I 820 TTAA T^TAA
TspRI 553 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 20 enzymes) #
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BbvI 355 GCAGC GCAGC(8/12)
595 GCTGC
BsmAI 633 GAGAC GTCTC(1/5)
426 GTCTC
BsmFI 436 GGGAC GGGAC(10/14)
884 GTCCC
BsmI 249 GAATGC GAATGC(1/-1)
542 GAATGC
Cfr13I 281 GGCCC G^G[ACGT]CC
306 GGCCC
DpnII 153 GATC ^GATC
381 GATC
Fnu4HI 355 GCAGC GC^[ACGT]GC
595 GCTGC
HaeIII 281 GGCC GG^CC
306 GGCC
HgaI 27 GACGC GACGC(5/10)
626 GCGTC
ItaI 355 GCAGC GC^[ACGT]GC
595 GCTGC
MaeII 318 ACGT A^CGT
350 ACGT
MaeIII 517 GTGAC ^GT[ACGT]AC
899 GTGAC
MboI 153 GATC ^GATC
381 GATC
MboII 696 GAAGA GAAGA(8/7)
763 GAAGA
MwoI 598 GCCACAATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
714 GCTTCAACTGC
NdeII 153 GATC ^GATC
381 GATC
Sau3AI 153 GATC ^GATC
381 GATC
Sau96I 281 GGCCC G^G[ACGT]CC
306 GGCCC
TseI 355 GCAGC G^C[AT]GC
595 GCTGC
Tsp45I 517 GTGAC ^GT[GC]AC
899 GTGAC
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AfaI AluI BsaJI BssKI BstF5I
BstNI Cac8I DdeI EcoRII FokI
HinfI HphI MnlI MvaI NlaIII
NspI PleI RsaI ScrFI TaqI
TfiI Tsp509I TthHB8I