Restriction map for
pFN21AE4076
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccIII AclI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII BalI BanII
BbrPI BbvCI BcgI BciVI BclI
BfrI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssSI BstBI BstEII
BstPI Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII KpnI
Ksp632I MaeII MamI MfeI MluI
MluNI MroI MscI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tth111I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 579 GTATAC GT^[AC][GT]AC
AcsI 945 GAATTT [GA]^AATT[CT]
Aor51HI 929 AGCGCT AGC^GCT
ApoI 945 GAATTT [GA]^AATT[CT]
AvrII 747 CCTAGG C^CTAGG
BamHI 751 GGATCC G^GATCC
BglI 17 GCCACGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 747 CCTAGG C^CTAGG
BmyI 478 GGGCAC G[AGT]GC[ACT]^C
BseRI 784 CTCCTC GAGGAG(10/8)
BsiEI 293 CGGCCG CG[GA][CT]^CG
BsmBI 418 GAGACG CGTCTC(1/5)
Bsp1286I 478 GGGCAC G[AGT]GC[ACT]^C
BspHI 897 TCATGA T^CATGA
BspMI 143 ACCTGC ACCTGC(4/8)
Bst1107I 579 GTATAC GTA^TAC
BstAPI 509 GCAAGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 468 CCAGCGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 579 GTATAC GTA^TAC
DraII 1141 AGGCCCC [GA]G^G[ACGT]CC[CT]
EagI 293 CGGCCG C^GGCCG
EclXI 293 CGGCCG C^GGCCG
Eco47III 929 AGCGCT AGC^GCT
Eco52I 293 CGGCCG C^GGCCG
EcoO109I 1141 AGGCCCC [GA]G^G[ACGT]CC[CT]
HincII 1083 GTTAAC GT[CT]^[GA]AC
HindII 1083 GTTAAC GT[CT]^[GA]AC
HpaI 1083 GTTAAC GTT^AAC
PflMI 279 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 589 CAGCTG CAG^CTG
RcaI 897 TCATGA T^CATGA
SmaI 355 CCCGGG CCC^GGG
TaqI 989 TCGA T^CGA
TthHB8I 989 TCGA T^CGA
Van91I 279 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 355 CCCGGG C^CCGGG
XmaI 355 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BbeI 320 GGCGCC GGCGC^C
400 GGCGCC
BbsI 1150 GAAGAC GAAGAC(2/6)
1011 GTCTTC
BglII 512 AGATCT A^GATCT
842 AGATCT
BpuAI 1150 GAAGAC GAAGAC(2/6)
1011 GTCTTC
BsgI 225 GTGCAG GTGCAG(16/14)
432 GTGCAG
BssHII 180 GCGCGC G^CGCGC
471 GCGCGC
DdeI 365 CTGAG C^T[ACGT]AG
1079 CTCAG
EcoNI 324 CCTCCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1088 CCTCACCAAGG
EcoT14I 747 CCTAGG C^C[AT][AT]GG
1093 CCAAGG
HphI 538 GGTGA GGTGA(8/7)
1090 TCACC
KasI 320 GGCGCC G^GCGCC
400 GGCGCC
MaeIII 775 GTGAC ^GT[ACGT]AC
1195 GTGAC
MslI 669 CATTAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
952 CACTGGGATG
NarI 320 GGCGCC GG^CGCC
400 GGCGCC
PstI 265 CTGCAG CTGCA^G
908 CTGCAG
SfcI 265 CTGCAG C^T[GA][CT]AG
908 CTGCAG
SfoI 320 GGCGCC GGC^GCC
400 GGCGCC
StyI 747 CCTAGG C^C[AT][AT]GG
1093 CCAAGG
Tsp45I 775 GTGAC ^GT[GC]AC
1195 GTGAC
Tsp509I 946 AATT ^AATT
967 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AvaI AvaII BanI BbvI BcnI
BfaI BsaHI BsaI BsaJI BsiYI
BslI BsmAI BsmFI BsoBI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DpnII DsaI EaeI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII ItaI KspI MaeI
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PleI SacII Sau3AI Sau96I
ScrFI Tru9I TseI TspRI XhoII