Restriction map for
pFN21AE1142
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AviII AvrII BalI BamHI
BcgI BciVI BclI BfrI BglI
BglII BlnI BsaBI BsaI BsaWI
BseAI BsiWI BsmI BspDI BspEI
BspHI BspMI BsrBI BsrDI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KspI MamI MfeI
MluNI MroI MscI MunI NcoI
NdeI NheI NotI NsiI NspV
PacI PaeR7I PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI TspRI XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 878 GGTACC G^GTACC
AcsI 1218 GAATTT [GA]^AATT[CT]
ApaI 187 GGGCCC GGGCC^C
ApoI 1218 GAATTT [GA]^AATT[CT]
Asp718I 878 GGTACC G^GTACC
AspI 1192 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 209 GGACC G^G[AT]CC
BbeI 115 GGCGCC GGCGC^C
BbrPI 613 CACGTG CAC^GTG
BbsI 1116 GAAGAC GAAGAC(2/6)
BbvCI 774 GCTGAGG CCTCAGC(-5/-2)
BlpI 475 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 475 GCTGAGC GC^T[ACGT]AGC
BpuAI 1116 GAAGAC GAAGAC(2/6)
BsgI 66 CTGCAC GTGCAG(16/14)
BsiEI 110 CGGCCG CG[GA][CT]^CG
BsmBI 544 GAGACG CGTCTC(1/5)
Bsp1407I 338 TGTACA T^GTACA
BspLU11I 620 ACATGT A^CATGT
BsrGI 338 TGTACA T^GTACA
BssHII 606 GCGCGC G^CGCGC
CelII 475 GCTGAGC GC^T[ACGT]AGC
DraI 1083 TTTAAA TTT^AAA
DraIII 69 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 110 CGGCCG C^GGCCG
EclXI 110 CGGCCG C^GGCCG
Eco52I 110 CGGCCG C^GGCCG
EcoT14I 746 CCAAGG C^C[AT][AT]GG
HincII 568 GTCAAC GT[CT]^[GA]AC
HindII 568 GTCAAC GT[CT]^[GA]AC
KasI 115 GGCGCC G^GCGCC
KpnI 878 GGTACC GGTAC^C
MluI 1253 ACGCGT A^CGCGT
NarI 115 GGCGCC GG^CGCC
PflFI 1192 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 153 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 613 CACGTG CAC^GTG
PmlI 613 CACGTG CAC^GTG
SacI 1045 GAGCTC GAGCT^C
ScaI 20 AGTACT AGT^ACT
SfoI 115 GGCGCC GGC^GCC
SmlI 1089 CTTGAG C^T[CT][GA]AG
SspBI 338 TGTACA T^GTACA
StuI 362 AGGCCT AGG^CCT
StyI 746 CCAAGG C^C[AT][AT]GG
TfiI 1157 GAATC G^A[AT]TC
Tth111I 1192 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 153 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 115 GGCGCC G[GA]^CG[CT]C
238 GACGCC
AflIII 620 ACATGT A^C[GA][CT]GT
1253 ACGCGT
AlwNI 226 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
484 CAGCAGCTG
AspHI 1045 GAGCTC G[AT]GC[AT]^C
1069 GTGCTC
AvaI 97 CCCGAG C^[CT]CG[GA]G
190 CCCGAG
BcnI 299 CCCGG CC^[GC]GG
852 CCGGG
BfaI 970 CTAG C^TAG
1181 CTAG
BpmI 1141 CTCCAG CTGGAG(16/14)
1245 CTCCAG
BsaHI 115 GGCGCC G[GA]^CG[CT]C
238 GACGCC
BseRI 220 GAGGAG GAGGAG(10/8)
751 GAGGAG
BsiHKAI 1045 GAGCTC G[AT]GC[AT]^C
1069 GTGCTC
BsmAI 544 GAGAC GTCTC(1/5)
645 GAGAC
BsmFI 1250 GGGAC GGGAC(10/14)
410 GTCCC
BsoBI 97 CCCGAG C^[CT]CG[GA]G
190 CCCGAG
BstAPI 68 GCACCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
225 GCAGCTGCTGC
BstYI 860 AGATCC [GA]^GATC[CT]
1241 GGATCT
DdeI 476 CTGAG C^T[ACGT]AG
775 CTGAG
DraII 186 GGGGCCC [GA]G^G[ACGT]CC[CT]
420 AGGCCCC
DsaI 144 CCACGG C^C[GA][CT]GG
345 CCACGG
EarI 907 GAAGAG CTCTTC(1/4)
948 GAAGAG
EcoO109I 186 GGGGCCC [GA]G^G[ACGT]CC[CT]
420 AGGCCCC
Hin1I 115 GGCGCC G[GA]^CG[CT]C
238 GACGCC
Ksp632I 907 GAAGAG CTCTTC(1/4)
948 GAAGAG
MaeI 970 CTAG C^TAG
1181 CTAG
MflI 860 AGATCC [GA]^GATC[CT]
1241 GGATCT
MseI 1084 TTAA T^TAA
1130 TTAA
NaeI 112 GCCGGC GCC^GGC
973 GCCGGC
NciI 299 CCCGG CC^[GC]GG
852 CCGGG
NgoMIV 112 GCCGGC G^CCGGC
973 GCCGGC
NruI 216 TCGCGA TCG^CGA
1059 TCGCGA
PstI 871 CTGCAG CTGCA^G
1074 CTGCAG
SfaNI 538 GATGC GCATC(5/9)
368 GCATC
SfcI 871 CTGCAG C^T[GA][CT]AG
1074 CTGCAG
Tru9I 1084 TTAA T^TAA
1130 TTAA
Tsp509I 1128 AATT ^AATT
1219 AATT
XhoII 860 AGATCC [GA]^GATC[CT]
1241 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BanI BanII BbvI BmyI BsaAI
BsaJI BsiYI BslI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DpnII
EaeI EcoNI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PleI PvuII RsaI Sau3AI
Sau96I ScrFI TaqI TseI Tsp45I
TthHB8I