Restriction map for
pFN21AE1925
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI Alw44I AlwNI
AocI ApaLI ApoI AscI AseI
AsnI Asp718I AspI AvrII BamHI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BsaAI
BsaBI BsaWI BseAI BsgI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI KspI MaeI MamI MfeI
MflI MluI MroI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuII
RcaI RsrII SacII SalI SbfI
SfiI SfuI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TfiI Tsp509I Tth111I Van91I
XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 685 GTCTAC GT^[AC][GT]AC
AhdI 760 GACGGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1133 AGCGCT AGC^GCT
AspEI 760 GACGGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 716 TGCGCA TGC^GCA
BlpI 729 GCTCAGC GC^T[ACGT]AGC
BpmI 1005 CTCCAG CTGGAG(16/14)
Bpu1102I 729 GCTCAGC GC^T[ACGT]AGC
BseRI 1365 GAGGAG GAGGAG(10/8)
BspMI 360 GCAGGT ACCTGC(4/8)
BsrFI 1076 GCCGGC [GA]^CCGG[CT]
BstAPI 1094 GCAGCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 729 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1076 GCCGGC [GA]^CCGG[CT]
Eam1105I 760 GACGGTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1133 AGCGCT AGC^GCT
FspI 716 TGCGCA TGC^GCA
HinfI 985 GAGTC G^A[ACGT]TC
MaeIII 457 GTGAC ^GT[ACGT]AC
MseI 1343 TTAA T^TAA
NaeI 1076 GCCGGC GCC^GGC
NgoMIV 1076 GCCGGC G^CCGGC
PleI 985 GAGTC GAGTC(4/5)
PpuMI 1009 AGGTCCC [GA]G^G[AT]CC[CT]
PvuI 413 CGATCG CGAT^CG
SacI 334 GAGCTC GAGCT^C
SapI 1251 GCTCTTC GCTCTTC(1/4)
ScaI 134 AGTACT AGT^ACT
SexAI 622 ACCTGGT A^CC[AT]GGT
SfcI 1203 CTACAG C^T[GA][CT]AG
SgrAI 1075 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1014 CCCGGG CCC^GGG
StuI 1229 AGGCCT AGG^CCT
Tru9I 1343 TTAA T^TAA
Tsp45I 457 GTGAC ^GT[GC]AC
XcmI 329 CCAACGAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1014 CCCGGG C^CCGGG
XmaI 1014 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwI 1337 GATCC GGATC(4/5)
1271 GGATC
Asp700I 258 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1131 GAAGCGCTTC
AspHI 334 GAGCTC G[AT]GC[AT]^C
1174 GTGCTC
AvaI 102 CTCGGG C^[CT]CG[GA]G
1014 CCCGGG
AvaII 441 GGACC G^G[AT]CC
1010 GGTCC
BbsI 187 GTCTTC GAAGAC(2/6)
1315 GTCTTC
BglI 100 GCCTCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1208 GCCTGTACGGC
BpuAI 187 GTCTTC GAAGAC(2/6)
1315 GTCTTC
BsaI 619 GAGACC GGTCTC(1/5)
561 GGTCTC
BsiHKAI 334 GAGCTC G[AT]GC[AT]^C
1174 GTGCTC
BsmAI 619 GAGAC GTCTC(1/5)
562 GTCTC
BsoBI 102 CTCGGG C^[CT]CG[GA]G
1014 CCCGGG
BstXI 431 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
509 CCACCATCGTGG
DdeI 730 CTCAG C^T[ACGT]AG
983 CTGAG
MaeII 206 ACGT A^CGT
845 ACGT
XmnI 258 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1131 GAAGCGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI BalI BanI BanII BbeI
BbvI BcnI BmyI BsaHI BsaJI
BsiEI BsiYI BslI BsmFI Bsp1286I
BsrBI BsrI BssHII BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI KasI Ksp632I
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SfoI TaqI TseI TspRI
TthHB8I