##################################
# NO CUT ENZYMES (  186 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaJI       BsaWI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmFI       BsmI        Bsp1286I    Bsp1407I    BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssKI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstNI       
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      CpoI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRII      
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MaeI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MspA1I      MspI        MunI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        ScrFI       
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

AciI           97  GCGG                 CCGC(-3/-1)
AfaI           78  GTAC                 GT^AC
AseI          385  ATTAAT               AT^TAAT
AsnI          385  ATTAAT               AT^TAAT
AvaI           56  CCCGAG               C^[CT]CG[GA]G
BseRI          16  GAGGAG               GAGGAG(10/8)
BsgI          244  GTGCAG               GTGCAG(16/14)
BsmAI         744  GAGAC                GTCTC(1/5)
BsoBI          56  CCCGAG               C^[CT]CG[GA]G
BspDI         251  ATCGAT               AT^CGAT
BsrI          451  CCAGT                ACTGG(1/-1)
ClaI          251  ATCGAT               AT^CGAT
DraI          169  TTTAAA               TTT^AAA
EcoRI         215  GAATTC               G^AATTC
FauI           97  GCGGG                CCCGC(4/6)
MaeII         242  ACGT                 A^CGT
NspI          438  GCATGT               [GA]CATG^[CT]
PshBI         385  ATTAAT               AT^TAAT
PstI          457  CTGCAG               CTGCA^G
RsaI           78  GTAC                 GT^AC
SapI          269  GCTCTTC              GCTCTTC(1/4)
SspI          410  AATATT               AAT^ATT
TaqI          252  TCGA                 T^CGA
TthHB8I       252  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

Asp700I        67  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              409  GAATATTTTC          
BbvI          459  GCAGC                GCAGC(8/12)
              456  GCTGC               
BstF5I        503  CATCC                GGATG(2/0)
              629  CATCC               
DdeI           11  CTCAG                C^T[ACGT]AG
              824  CTAAG               
EarI          270  CTCTTC               CTCTTC(1/4)
               67  GAAGAG              
Fnu4HI        456  GCTGC                GC^[ACGT]GC
              459  GCAGC               
FokI          503  CATCC                GGATG(9/13)
              629  CATCC               
HphI          570  TCACC                GGTGA(8/7)
              696  TCACC               
ItaI          456  GCTGC                GC^[ACGT]GC
              459  GCAGC               
Ksp632I       270  CTCTTC               CTCTTC(1/4)
               67  GAAGAG              
MslI          557  CATATAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              683  CATATAAATG          
SfcI          327  CTACAG               C^T[GA][CT]AG
              457  CTGCAG              
TseI          456  GCTGC                G^C[AT]GC
              459  GCAGC               
XmnI           67  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              409  GAATATTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AcsI        AluI        ApoI        AvaII       BbsI        
BglII       BpuAI       BsiYI       BslI        BstYI       
Cfr13I      DpnII       HaeIII      HinfI       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
NdeII       NlaIII      NlaIV       PleI        Sau3AI      
Sau96I      SfaNI       SmlI        TfiI        Tru9I       
Tsp45I      Tsp509I     TspRI       XhoII