Restriction map for
pFN21AE3116
##################################
# NO CUT ENZYMES ( 176 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BcgI BfaI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI Bsp1286I BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoT14I EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AciI 488 GCGG CCGC(-3/-1)
AlwI 463 GGATC GGATC(4/5)
Asp700I 12 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 477 GCTGAGG CCTCAGC(-5/-2)
BciVI 849 GGATAC GTATCC(6/5)
BclI 639 TGATCA T^GATCA
BcnI 36 CCCGG CC^[GC]GG
BpmI 130 CTGGAG CTGGAG(16/14)
BsaI 623 GAGACC GGTCTC(1/5)
BsmAI 623 GAGAC GTCTC(1/5)
BsmI 544 GCATTC GAATGC(1/-1)
Bsp1407I 687 TGTACA T^GTACA
BspDI 76 ATCGAT AT^CGAT
BspHI 642 TCATGA T^CATGA
BsrGI 687 TGTACA T^GTACA
BstAPI 310 GCAAATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 504 GGATG GGATG(2/0)
BstXI 299 CCAGAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 137 GCGAGC GC[ACGT]^[ACGT]GC
ClaI 76 ATCGAT AT^CGAT
DdeI 478 CTGAG C^T[ACGT]AG
DsaI 56 CCACGG C^C[GA][CT]GG
EaeI 53 CGGCCA [CT]^GGCC[GA]
EcoRV 238 GATATC GAT^ATC
FbaI 639 TGATCA T^GATCA
FokI 504 GGATG GGATG(9/13)
HapII 37 CCGG C^CGG
HpaII 37 CCGG C^CGG
MaeIII 404 GTCAC ^GT[ACGT]AC
MslI 406 CACAAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 486 CAGCGG C[AC]G^C[GT]G
MspI 37 CCGG C^CGG
NciI 36 CCCGG CC^[GC]GG
PstI 243 CTGCAG CTGCA^G
RcaI 642 TCATGA T^CATGA
SfaNI 646 GATGC GCATC(5/9)
SfcI 243 CTGCAG C^T[GA][CT]AG
SspBI 687 TGTACA T^GTACA
SspI 593 AATATT AAT^ATT
TaqI 77 TCGA T^CGA
Tsp45I 404 GTCAC ^GT[GC]AC
TthHB8I 77 TCGA T^CGA
XmnI 12 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AluI 278 AGCT AG^CT
399 AGCT
BsrI 129 ACTGG ACTGG(1/-1)
627 CCAGT
BstNI 445 CCTGG CC^[AT]GG
451 CCTGG
Cfr13I 39 GGCCC G^G[ACGT]CC
448 GGCCC
DpnII 464 GATC ^GATC
640 GATC
EarI 115 GAAGAG CTCTTC(1/4)
499 GAAGAG
EcoRII 445 CCTGG ^CC[AT]GG
451 CCTGG
HphI 709 GGTGA GGTGA(8/7)
175 TCACC
Ksp632I 115 GAAGAG CTCTTC(1/4)
499 GAAGAG
MboI 464 GATC ^GATC
640 GATC
MvaI 445 CCTGG CC^[AT]GG
451 CCTGG
MwoI 310 GCAAATGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
435 GCGAAAATGGC
NdeII 464 GATC ^GATC
640 GATC
Sau3AI 464 GATC ^GATC
640 GATC
Sau96I 39 GGCCC G^G[ACGT]CC
448 GGCCC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AcsI AfaI ApoI BbvI BsaJI
BsiYI BslI BssKI Fnu4HI HaeIII
HinfI ItaI MboII MnlI MseI
NlaIII PleI RsaI ScrFI TfiI
Tru9I TseI Tsp509I TspRI