Restriction map for
pFN21AE5639
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BfrI BlnI
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HpaI
HphI KasI KpnI KspI MamI
MfeI MluI MroI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NsiI NspI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp509I Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AatII 521 GACGTC GACGT^C
AcyI 521 GACGTC G[GA]^CG[CT]C
AfaI 45 GTAC GT^AC
AlwI 1566 GATCC GGATC(4/5)
AspHI 508 GAGCTC G[AT]GC[AT]^C
AvaI 460 CCCGAG C^[CT]CG[GA]G
BanI 1470 GGTGCC G^G[CT][GA]CC
BfaI 1393 CTAG C^TAG
BglI 736 GCCCGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 599 AGATCT A^GATCT
BsaHI 521 GACGTC G[GA]^CG[CT]C
BsiHKAI 508 GAGCTC G[AT]GC[AT]^C
BsoBI 460 CCCGAG C^[CT]CG[GA]G
BsrFI 84 GCCGGT [GA]^CCGG[CT]
BstAPI 636 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 121 TTCGAA TT^CGAA
BstYI 599 AGATCT [GA]^GATC[CT]
Cfr10I 84 GCCGGT [GA]^CCGG[CT]
HgaI 987 GACGC GACGC(5/10)
Hin1I 521 GACGTC G[GA]^CG[CT]C
HindIII 125 AAGCTT A^AGCTT
MaeI 1393 CTAG C^TAG
MflI 599 AGATCT [GA]^GATC[CT]
NruI 939 TCGCGA TCG^CGA
NspV 121 TTCGAA TT^CGAA
RsaI 45 GTAC GT^AC
SacI 508 GAGCTC GAGCT^C
SfuI 121 TTCGAA TT^CGAA
TfiI 714 GAATC G^A[AT]TC
XhoII 599 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 940 CGCG CG^CG
1445 CGCG
BbsI 448 GAAGAC GAAGAC(2/6)
684 GAAGAC
BbvCI 412 GCTGAGG CCTCAGC(-5/-2)
568 GCTGAGG
BlpI 486 GCTGAGC GC^T[ACGT]AGC
510 GCTCAGC
Bpu1102I 486 GCTGAGC GC^T[ACGT]AGC
510 GCTCAGC
BpuAI 448 GAAGAC GAAGAC(2/6)
684 GAAGAC
BsmI 80 GAATGC GAATGC(1/-1)
537 GAATGC
BsrBI 55 GAGCGG CCGCTC(-3/-3)
817 GAGCGG
BstUI 940 CGCG CG^CG
1445 CGCG
CelII 486 GCTGAGC GC^T[ACGT]AGC
510 GCTCAGC
DsaI 659 CCATGG C^C[GA][CT]GG
854 CCATGG
FauI 819 GCGGG CCCGC(4/6)
1449 GCGGG
HincII 323 GTCAAC GT[CT]^[GA]AC
1329 GTTGAC
HindII 323 GTCAAC GT[CT]^[GA]AC
1329 GTTGAC
MaeII 118 ACGT A^CGT
522 ACGT
MslI 395 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1319 CATCCATGTG
MvnI 940 CGCG CG^CG
1445 CGCG
NcoI 659 CCATGG C^CATGG
854 CCATGG
PpuMI 500 AGGACCT [GA]G^G[AT]CC[CT]
677 AGGACCT
XcmI 269 CCACTGTGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1633 CCACCTCCCACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AluI AlwNI AocI ApaI
AvaII BalI BanII BbvI BcnI
BmyI BpmI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MaeIII MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I