Restriction map for
pFN21AE4226
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# NO CUT ENZYMES ( 84 enzymes) #
##################################
AccI AccIII AclI AgeI Aor51HI
AscI AseI AsnI AviII AvrII
BalI BcgI BclI BglII BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BsrDI BsrGI Bst1107I BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII Eco47III EcoO65I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KspI MamI
MfeI MluNI MroI MscI MunI
NcoI NdeI NheI NotI NruI
NsiI PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI ScaI SexAI
SfiI SnaBI SpeI SphI SspBI
SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflII 2383 CTTAAG C^TTAAG
Asp700I 382 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 81 GGATCC G^GATCC
BbeI 1300 GGCGCC GGCGC^C
BbrPI 733 CACGTG CAC^GTG
BbsI 1081 GTCTTC GAAGAC(2/6)
BciVI 479 GTATCC GTATCC(6/5)
BfaI 352 CTAG C^TAG
BfrI 2383 CTTAAG C^TTAAG
BglI 2260 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1081 GTCTTC GAAGAC(2/6)
BsaAI 733 CACGTG [CT]AC^GT[GA]
BsiEI 540 CGGCCG CG[GA][CT]^CG
BspLU11I 1349 ACATGT A^CATGT
BssSI 684 CTCGTG CACGAG(-5/-1)
BstBI 49 TTCGAA TT^CGAA
BstYI 81 GGATCC [GA]^GATC[CT]
EagI 540 CGGCCG C^GGCCG
EclXI 540 CGGCCG C^GGCCG
Eco52I 540 CGGCCG C^GGCCG
EcoRI 2313 GAATTC G^AATTC
KasI 1300 GGCGCC G^GCGCC
MaeI 352 CTAG C^TAG
MflI 81 GGATCC [GA]^GATC[CT]
MluI 695 ACGCGT A^CGCGT
MslI 798 CATTGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1300 GGCGCC GG^CGCC
NspI 1349 ACATGT [GA]CATG^[CT]
NspV 49 TTCGAA TT^CGAA
PmaCI 733 CACGTG CAC^GTG
PmlI 733 CACGTG CAC^GTG
PshAI 1750 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 72 CCTGCAGG CCTGCA^GG
SfoI 1300 GGCGCC GGC^GCC
SfuI 49 TTCGAA TT^CGAA
SgrAI 1123 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 72 CCTGCAGG CCTGCA^GG
XcmI 2255 CCAAAGCCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 81 GGATCC [GA]^GATC[CT]
XmnI 382 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 850 GACGTC GACGT^C
2190 GACGTC
Acc65I 268 GGTACC G^GTACC
1025 GGTACC
AflIII 695 ACGCGT A^C[GA][CT]GT
1349 ACATGT
AhdI 922 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1909 GACTCCAGGTC
Alw44I 805 GTGCAC G^TGCAC
1135 GTGCAC
AocI 1244 CCTCAGG CC^T[ACGT]AGG
1600 CCTGAGG
ApaLI 805 GTGCAC G^TGCAC
1135 GTGCAC
Asp718I 268 GGTACC G^GTACC
1025 GGTACC
AspEI 922 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1909 GACTCCAGGTC
AspI 361 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1414 GACTCTGTC
BbvCI 291 CCTCAGC CCTCAGC(-5/-2)
936 GCTGAGG
BsrBI 1356 CCGCTC CCGCTC(-3/-3)
1464 CCGCTC
BstAPI 135 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
906 GCAGGCGCTGC
Bsu36I 1244 CCTCAGG CC^T[ACGT]AGG
1600 CCTGAGG
DrdI 1033 GACGCACTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1942 GACCAAGAAGTC
DsaI 1618 CCGTGG C^C[GA][CT]GG
2290 CCACGG
Eam1105I 922 GACGTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1909 GACTCCAGGTC
Eco81I 1244 CCTCAGG CC^T[ACGT]AGG
1600 CCTGAGG
EcoNI 72 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
351 CCTAGCCCAGG
EcoT14I 304 CCTTGG C^C[AT][AT]GG
881 CCTTGG
KpnI 268 GGTACC GGTAC^C
1025 GGTACC
MseI 896 TTAA T^TAA
2384 TTAA
NaeI 229 GCCGGC GCC^GGC
2363 GCCGGC
NgoMIV 229 GCCGGC G^CCGGC
2363 GCCGGC
PflFI 361 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1414 GACTCTGTC
SapI 1014 GCTCTTC GCTCTTC(1/4)
1898 GCTCTTC
SmlI 439 CTTGAG C^T[CT][GA]AG
2383 CTTAAG
StuI 425 AGGCCT AGG^CCT
1115 AGGCCT
StyI 304 CCTTGG C^C[AT][AT]GG
881 CCTTGG
Tru9I 896 TTAA T^TAA
2384 TTAA
Tth111I 361 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1414 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XmaCI XmaI