Restriction map for
pFN21AE3678
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BseAI
BsgI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrDI BsrGI BsrI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NcoI NdeI NheI NruI
NsiI NspI NspV PacI PflFI
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XcmI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 353 GGTACC G^GTACC
AcyI 313 GGCGCC G[GA]^CG[CT]C
AgeI 216 ACCGGT A^CCGGT
Alw44I 435 GTGCAC G^TGCAC
AlwNI 706 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 205 GGGCCC GGGCC^C
ApaLI 435 GTGCAC G^TGCAC
Asp718I 353 GGTACC G^GTACC
BbeI 313 GGCGCC GGCGC^C
BbsI 472 GAAGAC GAAGAC(2/6)
BpuAI 472 GAAGAC GAAGAC(2/6)
BsaAI 229 TACGTG [CT]AC^GT[GA]
BsaHI 313 GGCGCC G[GA]^CG[CT]C
BsaWI 216 ACCGGT [AT]^CCGG[AT]
BsiEI 29 CGGCCG CG[GA][CT]^CG
BspHI 491 TCATGA T^CATGA
BstF5I 360 GGATG GGATG(2/0)
BstXI 302 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 130 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 6 CCACGG C^C[GA][CT]GG
EaeI 29 CGGCCG [CT]^GGCC[GA]
EagI 29 CGGCCG C^GGCCG
EarI 568 GAAGAG CTCTTC(1/4)
EclXI 29 CGGCCG C^GGCCG
Eco52I 29 CGGCCG C^GGCCG
EcoO109I 130 GGGTCCC [GA]G^G[ACGT]CC[CT]
FokI 360 GGATG GGATG(9/13)
Hin1I 313 GGCGCC G[GA]^CG[CT]C
KasI 313 GGCGCC G^GCGCC
KpnI 353 GGTACC GGTAC^C
Ksp632I 568 GAAGAG CTCTTC(1/4)
MaeII 230 ACGT A^CGT
MspA1I 349 CAGCGG C[AC]G^C[GT]G
NaeI 39 GCCGGC GCC^GGC
NarI 313 GGCGCC GG^CGCC
NgoMIV 39 GCCGGC G^CCGGC
NotI 28 GCGGCCGC GC^GGCCGC
PaeR7I 477 CTCGAG C^TCGAG
PinAI 216 ACCGGT A^CCGGT
PleI 475 GACTC GAGTC(4/5)
PpuMI 130 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 491 TCATGA T^CATGA
SacI 480 GAGCTC GAGCT^C
SfoI 313 GGCGCC GGC^GCC
SgrAI 38 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 477 CTCGAG C^T[CT][GA]AG
TfiI 192 GATTC G^A[AT]TC
XhoI 477 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaI 292 CCCGAG C^[CT]CG[GA]G
477 CTCGAG
BglI 34 GCCGCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
198 GCCGAATGGGC
BsoBI 292 CCCGAG C^[CT]CG[GA]G
477 CTCGAG
BsrBI 247 GAGCGG CCGCTC(-3/-3)
724 GAGCGG
BssHII 686 GCGCGC G^CGCGC
688 GCGCGC
BssSI 136 CACGAG CACGAG(-5/-1)
892 CACGAG
EcoNI 357 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
924 CCTGGCCCAGG
EcoT14I 170 CCAAGG C^C[AT][AT]GG
209 CCAAGG
HgaI 732 GACGC GACGC(5/10)
696 GCGTC
HinfI 192 GATTC G^A[ACGT]TC
475 GACTC
MaeIII 82 GTGAC ^GT[ACGT]AC
220 GTCAC
MslI 300 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
514 CACAAGGGTG
SfaNI 306 GATGC GCATC(5/9)
911 GCATC
StyI 170 CCAAGG C^C[AT][AT]GG
209 CCAAGG
Tsp45I 82 GTGAC ^GT[GC]AC
220 GTCAC
TspRI 564 CACTG CA[GC]TG[ACGT][ACGT]^
794 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrFI BssKI BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DpnII
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboI MboII MflI MnlI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PstI RsaI
Sau3AI Sau96I ScrFI SfcI TaqI
TseI TthHB8I XhoII