Restriction map for
pFN21AE2531
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccI AccIII AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MluI
MroI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshBI PvuI PvuII RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 494 CGCG CG^CG
AclI 287 AACGTT AA^CGTT
AflIII 304 ACATGT A^C[GA][CT]GT
AvaI 1946 CCCGAG C^[CT]CG[GA]G
AvaII 99 GGACC G^G[AT]CC
BalI 1468 TGGCCA TGG^CCA
BanII 2058 GAGCTC G[GA]GC[CT]^C
BcnI 504 CCGGG CC^[GC]GG
BseRI 765 CTCCTC GAGGAG(10/8)
BsmAI 1949 GAGAC GTCTC(1/5)
BsoBI 1946 CCCGAG C^[CT]CG[GA]G
BspLU11I 304 ACATGT A^CATGT
BspMI 1883 GCAGGT ACCTGC(4/8)
BsrDI 1962 CATTGC GCAATG(2/0)
BstAPI 362 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 494 CGCG CG^CG
BstYI 138 GGATCT [GA]^GATC[CT]
DraIII 1232 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1951 GACACCATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1000 CCTAAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 536 GAATTC G^AATTC
EcoRV 115 GATATC GAT^ATC
FauI 393 GCGGG CCCGC(4/6)
HapII 504 CCGG C^CGG
HgaI 1211 GACGC GACGC(5/10)
HpaII 504 CCGG C^CGG
MaeII 288 ACGT A^CGT
MfeI 668 CAATTG C^AATTG
MflI 138 GGATCT [GA]^GATC[CT]
MluNI 1468 TGGCCA TGG^CCA
MscI 1468 TGGCCA TGG^CCA
MslI 969 CACAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1543 CAGCGG C[AC]G^C[GT]G
MspI 504 CCGG C^CGG
MunI 668 CAATTG C^AATTG
MvnI 494 CGCG CG^CG
NciI 504 CCGGG CC^[GC]GG
NdeI 227 CATATG CA^TATG
PshAI 666 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 287 AACGTT AA^CGTT
PstI 1088 CTGCAG CTGCA^G
SacI 2058 GAGCTC GAGCT^C
SapI 40 GCTCTTC GCTCTTC(1/4)
SmlI 774 CTTGAG C^T[CT][GA]AG
SphI 2094 GCATGC GCATG^C
StuI 1603 AGGCCT AGG^CCT
XbaI 234 TCTAGA T^CTAGA
XhoII 138 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AfaI 996 GTAC GT^AC
1911 GTAC
AlwNI 363 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1015 CAGTGCCTG
AspHI 553 GAGCAC G[AT]GC[AT]^C
2058 GAGCTC
AspI 511 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1846 GACGGTGTC
BanI 1277 GGTGCC G^G[CT][GA]CC
1886 GGTGCC
BsaBI 1507 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1998 GATGCTGATC
BsgI 1854 CTGCAC GTGCAG(16/14)
1881 GTGCAG
BsiHKAI 553 GAGCAC G[AT]GC[AT]^C
2058 GAGCTC
BsmI 255 GAATGC GAATGC(1/-1)
643 GCATTC
BssSI 454 CACGAG CACGAG(-5/-1)
46 CTCGTG
BstXI 915 CCAAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1471 CCATGTCCTTGG
EaeI 705 CGGCCA [CT]^GGCC[GA]
1468 TGGCCA
EcoT22I 278 ATGCAT ATGCA^T
934 ATGCAT
MamI 1507 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1998 GATGCTGATC
NsiI 278 ATGCAT ATGCA^T
934 ATGCAT
NspI 304 ACATGT [GA]CATG^[CT]
2094 GCATGC
PflFI 511 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1846 GACGGTGTC
PflMI 465 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
708 CCAACGAATGG
RsaI 996 GTAC GT^AC
1911 GTAC
Tth111I 511 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1846 GACGGTGTC
Van91I 465 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
708 CCAACGAATGG
XcmI 968 CCACAGCAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1431 CCACTTGATCTCTGG
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbsI BbvI BclI BfaI BmyI
BpmI BpuAI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I