Restriction map for
pFN21AE5794
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccII AccIII AflII
AflIII AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvrII BbrPI BcgI
BciVI BclI BfrI BglI BglII
BlnI BsaAI BsaBI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII BstBI BstEII BstPI
BstUI ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI KspI MamI MluI MroI
MvnI NcoI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SacI SacII SalI
SbfI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StyI SwaI TfiI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 717 GTATAC GT^[AC][GT]AC
AclI 324 AACGTT AA^CGTT
AcsI 831 AAATTT [GA]^AATT[CT]
AcyI 1414 GGCGCC G[GA]^CG[CT]C
AfaI 647 GTAC GT^AC
AgeI 214 ACCGGT A^CCGGT
AocI 766 CCTCAGG CC^T[ACGT]AGG
ApoI 831 AAATTT [GA]^AATT[CT]
Asp700I 28 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1481 TGCGCA TGC^GCA
BamHI 1840 GGATCC G^GATCC
BbeI 1414 GGCGCC GGCGC^C
BbsI 339 GTCTTC GAAGAC(2/6)
BbvCI 202 GCTGAGG CCTCAGC(-5/-2)
BfaI 1520 CTAG C^TAG
BlpI 388 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 388 GCTCAGC GC^T[ACGT]AGC
BpuAI 339 GTCTTC GAAGAC(2/6)
BsaHI 1414 GGCGCC G[GA]^CG[CT]C
BsgI 1330 GTGCAG GTGCAG(16/14)
BsiEI 1526 CGGTCG CG[GA][CT]^CG
BsmI 28 GAATGC GAATGC(1/-1)
BsrBI 1497 GAGCGG CCGCTC(-3/-3)
BsrDI 1234 GCAATG GCAATG(2/0)
BssSI 821 CACGAG CACGAG(-5/-1)
Bst1107I 717 GTATAC GTA^TAC
BstZ17I 717 GTATAC GTA^TAC
Bsu36I 766 CCTCAGG CC^T[ACGT]AGG
CelII 388 GCTCAGC GC^T[ACGT]AGC
DraIII 517 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1000 GACCTTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 766 CCTCAGG CC^T[ACGT]AGG
EcoNI 736 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1481 TGCGCA TGC^GCA
HgaI 337 GCGTC GACGC(5/10)
Hin1I 1414 GGCGCC G[GA]^CG[CT]C
KasI 1414 GGCGCC G^GCGCC
MaeI 1520 CTAG C^TAG
MfeI 628 CAATTG C^AATTG
MunI 628 CAATTG C^AATTG
NaeI 1878 GCCGGC GCC^GGC
NarI 1414 GGCGCC GG^CGCC
NdeI 527 CATATG CA^TATG
NgoMIV 1878 GCCGGC G^CCGGC
PinAI 214 ACCGGT A^CCGGT
Psp1406I 324 AACGTT AA^CGTT
RsaI 647 GTAC GT^AC
SapI 1869 GAAGAGC GCTCTTC(1/4)
ScaI 646 AGTACT AGT^ACT
SexAI 1256 ACCTGGT A^CC[AT]GGT
SfoI 1414 GGCGCC GGC^GCC
SmlI 1003 CTTGAG C^T[CT][GA]AG
TaqI 1610 TCGA T^CGA
TthHB8I 1610 TCGA T^CGA
XmnI 28 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
BalI 1644 TGGCCA TGG^CCA
1942 TGGCCA
BanII 1296 GGGCTC G[GA]GC[CT]^C
1890 GGGCTC
BsaWI 214 ACCGGT [AT]^CCGG[AT]
1924 TCCGGT
BspHI 12 TCATGA T^CATGA
1406 TCATGA
BspMI 708 ACCTGC ACCTGC(4/8)
742 GCAGGT
BsrFI 214 ACCGGT [GA]^CCGG[CT]
1878 GCCGGC
BstAPI 793 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1987 GCACGGGCTGC
BstXI 1229 CCAAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1548 CCAATGGCCTGG
BstYI 1304 AGATCC [GA]^GATC[CT]
1840 GGATCC
Cfr10I 214 ACCGGT [GA]^CCGG[CT]
1878 GCCGGC
DpnII 1305 GATC ^GATC
1841 GATC
DsaI 952 CCACGG C^C[GA][CT]GG
1176 CCGTGG
EaeI 1644 TGGCCA [CT]^GGCC[GA]
1942 TGGCCA
EarI 1687 CTCTTC CTCTTC(1/4)
1869 GAAGAG
HaeII 1024 GGCGCT [GA]GCGC^[CT]
1414 GGCGCC
Ksp632I 1687 CTCTTC CTCTTC(1/4)
1869 GAAGAG
MaeII 325 ACGT A^CGT
1328 ACGT
MboI 1305 GATC ^GATC
1841 GATC
MflI 1304 AGATCC [GA]^GATC[CT]
1840 GGATCC
MluNI 1644 TGGCCA TGG^CCA
1942 TGGCCA
MscI 1644 TGGCCA TGG^CCA
1942 TGGCCA
MseI 329 TTAA T^TAA
593 TTAA
NdeII 1305 GATC ^GATC
1841 GATC
NspI 239 GCATGC [GA]CATG^[CT]
1970 GCATGC
PflMI 888 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1011 CCAGCGGGTGG
PstI 409 CTGCAG CTGCA^G
1148 CTGCAG
RcaI 12 TCATGA T^CATGA
1406 TCATGA
Sau3AI 1305 GATC ^GATC
1841 GATC
SphI 239 GCATGC GCATG^C
1970 GCATGC
Tru9I 329 TTAA T^TAA
593 TTAA
Tsp45I 61 GTGAC ^GT[GC]AC
1856 GTGAC
Van91I 888 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1011 CCAGCGGGTGG
XhoII 1304 AGATCC [GA]^GATC[CT]
1840 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaI AvaII BanI BbvI BcnI
BmyI BpmI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboII MnlI MslI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
PleI PpuMI PvuII Sau96I ScrFI
SfaNI SfcI StuI TseI Tsp509I
TspRI XcmI