Restriction map for
pFN21AE2040
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
ApaLI ApoI AscI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BbeI BbrPI BbvCI BcgI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsiEI BsiWI
BsmI BsoBI BspDI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstUI BstXI CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco52I
EcoNI EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
PvuII RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I Van91I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcyI 210 GACGCC G[GA]^CG[CT]C
AflIII 1048 ACATGT A^C[GA][CT]GT
Aor51HI 485 AGCGCT AGC^GCT
ApaI 1040 GGGCCC GGGCC^C
Asp700I 827 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1308 GAGCTC G[AT]GC[AT]^C
BamHI 954 GGATCC G^GATCC
BanI 1239 GGCACC G^G[CT][GA]CC
BbsI 1490 GTCTTC GAAGAC(2/6)
BciVI 1513 GTATCC GTATCC(6/5)
BcnI 77 CCGGG CC^[GC]GG
BpmI 1508 CTCCAG CTGGAG(16/14)
BpuAI 1490 GTCTTC GAAGAC(2/6)
BsaHI 210 GACGCC G[GA]^CG[CT]C
BseRI 1752 CTCCTC GAGGAG(10/8)
BsiHKAI 1308 GAGCTC G[AT]GC[AT]^C
BsiYI 980 CCAGCCTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 980 CCAGCCTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1407I 1195 TGTACA T^GTACA
BspHI 206 TCATGA T^CATGA
BspLU11I 1048 ACATGT A^CATGT
BsrGI 1195 TGTACA T^GTACA
Bst1107I 1746 GTATAC GTA^TAC
BstYI 954 GGATCC [GA]^GATC[CT]
BstZ17I 1746 GTATAC GTA^TAC
CfoI 486 GCGC GCG^C
Eco47III 485 AGCGCT AGC^GCT
FauI 1517 CCCGC CCCGC(4/6)
HaeII 485 AGCGCT [GA]GCGC^[CT]
HaeIII 1041 GGCC GG^CC
HhaI 486 GCGC GCG^C
Hin1I 210 GACGCC G[GA]^CG[CT]C
HinP1I 486 GCGC G^CGC
HincII 945 GTTGAC GT[CT]^[GA]AC
HindII 945 GTTGAC GT[CT]^[GA]AC
MaeII 236 ACGT A^CGT
MflI 954 GGATCC [GA]^GATC[CT]
MslI 1086 CATTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1685 CCGCTG C[AC]G^C[GT]G
NciI 77 CCGGG CC^[GC]GG
NdeI 1145 CATATG CA^TATG
NspI 1048 ACATGT [GA]CATG^[CT]
PacI 931 TTAATTAA TTAAT^TAA
PleI 1292 GAGTC GAGTC(4/5)
PshAI 475 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 206 TCATGA T^CATGA
SacI 1308 GAGCTC GAGCT^C
SmlI 1305 CTTGAG C^T[CT][GA]AG
SspBI 1195 TGTACA T^GTACA
TfiI 909 GAATC G^A[AT]TC
XbaI 922 TCTAGA T^CTAGA
XhoII 954 GGATCC [GA]^GATC[CT]
XmnI 827 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1540 GTAGAC GT^[AC][GT]AC
1746 GTATAC
AccIII 399 TCCGGA T^CCGGA
912 TCCGGA
AocI 984 CCTAAGG CC^T[ACGT]AGG
1860 CCTAAGG
AseI 748 ATTAAT AT^TAAT
1555 ATTAAT
AsnI 748 ATTAAT AT^TAAT
1555 ATTAAT
BanII 1040 GGGCCC G[GA]GC[CT]^C
1308 GAGCTC
BclI 1710 TGATCA T^GATCA
1770 TGATCA
BfaI 466 CTAG C^TAG
923 CTAG
BsaWI 399 TCCGGA [AT]^CCGG[AT]
912 TCCGGA
BseAI 399 TCCGGA T^CCGGA
912 TCCGGA
BsgI 532 GTGCAG GTGCAG(16/14)
1781 GTGCAG
BsmBI 1334 CGTCTC CGTCTC(1/5)
1730 CGTCTC
BsmFI 342 GGGAC GGGAC(10/14)
565 GGGAC
BspEI 399 TCCGGA T^CCGGA
912 TCCGGA
BstEII 301 GGTCACC G^GT[ACGT]ACC
694 GGTTACC
BstPI 301 GGTCACC G^GT[ACGT]ACC
694 GGTTACC
Bsu36I 984 CCTAAGG CC^T[ACGT]AGG
1860 CCTAAGG
DsaI 551 CCATGG C^C[GA][CT]GG
836 CCATGG
Eco81I 984 CCTAAGG CC^T[ACGT]AGG
1860 CCTAAGG
EcoO65I 301 GGTCACC G^GT[ACGT]ACC
694 GGTTACC
EcoT14I 551 CCATGG C^C[AT][AT]GG
836 CCATGG
FbaI 1710 TGATCA T^GATCA
1770 TGATCA
HinfI 909 GAATC G^A[ACGT]TC
1292 GAGTC
MaeI 466 CTAG C^TAG
923 CTAG
MroI 399 TCCGGA T^CCGGA
912 TCCGGA
NcoI 551 CCATGG C^CATGG
836 CCATGG
PpuMI 342 GGGACCT [GA]G^G[AT]CC[CT]
1435 AGGACCT
PshBI 748 ATTAAT AT^TAAT
1555 ATTAAT
SapI 1417 GAAGAGC GCTCTTC(1/4)
382 GCTCTTC
StyI 551 CCATGG C^C[AT][AT]GG
836 CCATGG
TaqI 492 TCGA T^CGA
1295 TCGA
TspRI 9 CAGTG CA[GC]TG[ACGT][ACGT]^
963 CAGTG
TthHB8I 492 TCGA T^CGA
1295 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BmyI BsaJI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HapII
HgaI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI Tru9I
TseI Tsp45I Tsp509I