##################################
# NO CUT ENZYMES (  194 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BclI        
BfrI        BglI        BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmBI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DdeI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HpaI        HphI        KasI        
KpnI        KspI        MaeII       MamI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MvnI        MwoI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AvaII         239  GGACC                G^G[AT]CC
BbvI            4  GCTGC                GCAGC(8/12)
BcnI          236  CCCGG                CC^[GC]GG
BglII         547  AGATCT               A^GATCT
BsaI          500  GAGACC               GGTCTC(1/5)
BsmAI         500  GAGAC                GTCTC(1/5)
BsmFI         424  GTCCC                GGGAC(10/14)
BsrDI          43  GCAATG               GCAATG(2/0)
BsrI           13  CCAGT                ACTGG(1/-1)
BstF5I        531  GGATG                GGATG(2/0)
BstYI         547  AGATCT               [GA]^GATC[CT]
Cfr13I        239  GGACC                G^G[ACGT]CC
DpnII         548  GATC                 ^GATC
DraI          508  TTTAAA               TTT^AAA
EarI          166  CTCTTC               CTCTTC(1/4)
EcoT14I       177  CCAAGG               C^C[AT][AT]GG
Fnu4HI          4  GCTGC                GC^[ACGT]GC
FokI          531  GGATG                GGATG(9/13)
HaeIII        188  GGCC                 GG^CC
HapII         237  CCGG                 C^CGG
HindIII       118  AAGCTT               A^AGCTT
HpaII         237  CCGG                 C^CGG
ItaI            4  GCTGC                GC^[ACGT]GC
Ksp632I       166  CTCTTC               CTCTTC(1/4)
MboI          548  GATC                 ^GATC
MfeI           24  CAATTG               C^AATTG
MflI          547  AGATCT               [GA]^GATC[CT]
MspI          237  CCGG                 C^CGG
MunI           24  CAATTG               C^AATTG
NciI          236  CCCGG                CC^[GC]GG
NdeII         548  GATC                 ^GATC
NlaIII        433  CATG                 CATG^
Sau3AI        548  GATC                 ^GATC
Sau96I        239  GGACC                G^G[ACGT]CC
SfcI          219  CTGTAG               C^T[GA][CT]AG
StyI          177  CCAAGG               C^C[AT][AT]GG
TseI            4  GCTGC                G^C[AT]GC
Tsp45I        398  GTGAC                ^GT[GC]AC
TspRI         561  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoII         547  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

BbsI           19  GAAGAC               GAAGAC(2/6)
              574  GAAGAC              
BciVI         387  GGATAC               GTATCC(6/5)
              472  GGATAC              
BfaI          278  CTAG                 C^TAG
              481  CTAG                
BpuAI          19  GAAGAC               GAAGAC(2/6)
              574  GAAGAC              
BstNI          96  CCTGG                CC^[AT]GG
              427  CCAGG               
Cac8I         116  GCAAGC               GC[ACGT]^[ACGT]GC
              272  GCCAGC              
EcoRII         96  CCTGG                ^CC[AT]GG
              427  CCAGG               
MaeI          278  CTAG                 C^TAG
              481  CTAG                
MaeIII        292  GTAAC                ^GT[ACGT]AC
              398  GTGAC               
MseI          356  TTAA                 T^TAA
              509  TTAA                
MvaI           96  CCTGG                CC^[AT]GG
              427  CCAGG               
SfaNI         444  GATGC                GCATC(5/9)
               29  GCATC               
Tru9I         356  TTAA                 T^TAA
              509  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AcsI        AluI        ApoI        BsaJI       BssKI       
HinfI       MboII       MnlI        ScrFI       TfiI        
Tsp509I