Restriction map for
pFN21AB5753
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRV EcoT14I FauI
FbaI FseI FspI HaeII Hin1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tth111I TthHB8I Van91I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 45 CGCG CG^CG
AciI 44 CCGC CCGC(-3/-1)
AflIII 1280 ACACGT A^C[GA][CT]GT
AvaII 2010 GGACC G^G[AT]CC
BalI 1407 TGGCCA TGG^CCA
BanII 1140 GAGCCC G[GA]GC[CT]^C
BciVI 1380 GGATAC GTATCC(6/5)
BcnI 62 CCGGG CC^[GC]GG
BglI 43 GCCGCGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1949 CTCCAG CTGGAG(16/14)
BsaI 256 GAGACC GGTCTC(1/5)
BsaJI 1695 CCCTGG C^C[ACGT][ACGT]GG
BsgI 17 CTGCAC GTGCAG(16/14)
BsmFI 232 GGGAC GGGAC(10/14)
BstAPI 2218 GCAGCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 6 GGTTACC G^GT[ACGT]ACC
BstPI 6 GGTTACC G^GT[ACGT]ACC
BstUI 45 CGCG CG^CG
Cfr13I 2010 GGACC G^G[ACGT]CC
EaeI 1407 TGGCCA [CT]^GGCC[GA]
EcoO65I 6 GGTTACC G^GT[ACGT]ACC
EcoT22I 1457 ATGCAT ATGCA^T
HapII 62 CCGG C^CGG
HgaI 46 GCGTC GACGC(5/10)
HpaII 62 CCGG C^CGG
HphI 1174 GGTGA GGTGA(8/7)
MaeII 1282 ACGT A^CGT
MluNI 1407 TGGCCA TGG^CCA
MscI 1407 TGGCCA TGG^CCA
MspI 62 CCGG C^CGG
MvnI 45 CGCG CG^CG
NciI 62 CCGGG CC^[GC]GG
NsiI 1457 ATGCAT ATGCA^T
NspI 343 GCATGC [GA]CATG^[CT]
PshAI 1693 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1810 CTGCAG CTGCA^G
SapI 201 GAAGAGC GCTCTTC(1/4)
Sau96I 2010 GGACC G^G[ACGT]CC
SpeI 1326 ACTAGT A^CTAGT
SphI 343 GCATGC GCATG^C
XbaI 451 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Alw44I 838 GTGCAC G^TGCAC
1048 GTGCAC
AlwI 517 GATCC GGATC(4/5)
118 GGATC
ApaLI 838 GTGCAC G^TGCAC
1048 GTGCAC
AseI 937 ATTAAT AT^TAAT
1732 ATTAAT
AsnI 937 ATTAAT AT^TAAT
1732 ATTAAT
BsiYI 305 CCATTTTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
359 CCAGTGTTAGG
BslI 305 CCATTTTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
359 CCAGTGTTAGG
CfoI 97 GCGC GCG^C
1288 GCGC
HhaI 97 GCGC GCG^C
1288 GCGC
HinP1I 97 GCGC G^CGC
1288 GCGC
HincII 1674 GTTGAC GT[CT]^[GA]AC
2080 GTTGAC
HindII 1674 GTTGAC GT[CT]^[GA]AC
2080 GTTGAC
MspA1I 955 CAGCTG C[AC]G^C[GT]G
2090 CAGCTG
PshBI 937 ATTAAT AT^TAAT
1732 ATTAAT
PvuII 955 CAGCTG CAG^CTG
2090 CAGCTG
Tsp45I 1157 GTGAC ^GT[GC]AC
1451 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AcsI AfaI AluI ApoI AspHI
BbsI BbvI BfaI BglII BmyI
BpuAI BsiHKAI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
DdeI DpnII EarI EcoRI EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI ScaI ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp509I TspRI
XhoII