Restriction map for
pFN21AE3425
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
Acc65I AclI AcsI AflII AhdI
AlwI AocI ApoI AseI AsnI
Asp700I Asp718I AspEI AvrII BalI
BamHI BbrPI BciVI BclI BfrI
BglII BlnI BsaAI BsaBI BsaI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FbaI
HindIII HpaI KpnI MamI MfeI
MflI MluNI MscI MseI MunI
NdeI NruI NsiI NspI NspV
PacI PaeR7I PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SapI SbfI ScaI
SexAI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 681 GACGTC GACGT^C
AccI 1159 GTCGAC GT^[AC][GT]AC
AccIII 1614 TCCGGA T^CCGGA
AgeI 126 ACCGGT A^CCGGT
Aor51HI 796 AGCGCT AGC^GCT
AscI 1372 GGCGCGCC GG^CGCGCC
AspI 681 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 344 TGCGCA TGC^GCA
BbsI 466 GTCTTC GAAGAC(2/6)
BbvCI 1386 CCTCAGC CCTCAGC(-5/-2)
BcgI 296 GCACGCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1293 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1293 GCTGAGC GC^T[ACGT]AGC
BpuAI 466 GTCTTC GAAGAC(2/6)
BseAI 1614 TCCGGA T^CCGGA
BsgI 1030 GTGCAG GTGCAG(16/14)
BspEI 1614 TCCGGA T^CCGGA
BsrI 1334 CCAGT ACTGG(1/-1)
BstAPI 1126 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 441 CATCC GGATG(2/0)
BstXI 1056 CCACCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1293 GCTGAGC GC^T[ACGT]AGC
DpnII 1519 GATC ^GATC
EarI 883 GAAGAG CTCTTC(1/4)
Eco47III 796 AGCGCT AGC^GCT
EcoRV 952 GATATC GAT^ATC
FokI 441 CATCC GGATG(9/13)
FseI 1077 GGCCGGCC GGCCGG^CC
FspI 344 TGCGCA TGC^GCA
Ksp632I 883 GAAGAG CTCTTC(1/4)
MboI 1519 GATC ^GATC
MroI 1614 TCCGGA T^CCGGA
NdeII 1519 GATC ^GATC
NheI 841 GCTAGC G^CTAGC
NotI 109 GCGGCCGC GC^GGCCGC
PflFI 681 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 225 CCATGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 126 ACCGGT A^CCGGT
PpuMI 498 GGGACCT [GA]G^G[AT]CC[CT]
SalI 1159 GTCGAC G^TCGAC
Sau3AI 1519 GATC ^GATC
SmlI 244 CTCAAG C^T[CT][GA]AG
TfiI 859 GAATC G^A[AT]TC
Tth111I 681 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 225 CCATGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1460 CCACTGCCGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 189 GTAC GT^AC
1590 GTAC
AflIII 963 ACGCGT A^C[GA][CT]GT
1142 ACGCGT
Alw44I 294 GTGCAC G^TGCAC
1558 GTGCAC
ApaLI 294 GTGCAC G^TGCAC
1558 GTGCAC
AspHI 294 GTGCAC G[AT]GC[AT]^C
1558 GTGCAC
BfaI 754 CTAG C^TAG
842 CTAG
BpmI 503 CTGGAG CTGGAG(16/14)
994 CTGGAG
BseRI 364 GAGGAG GAGGAG(10/8)
820 GAGGAG
BsiHKAI 294 GTGCAC G[AT]GC[AT]^C
1558 GTGCAC
HincII 1159 GTCGAC GT[CT]^[GA]AC
1603 GTCAAC
HindII 1159 GTCGAC GT[CT]^[GA]AC
1603 GTCAAC
MaeI 754 CTAG C^TAG
842 CTAG
MaeII 682 ACGT A^CGT
1025 ACGT
MaeIII 1337 GTCAC ^GT[ACGT]AC
1529 GTGAC
MboII 883 GAAGA GAAGA(8/7)
467 TCTTC
MluI 963 ACGCGT A^CGCGT
1142 ACGCGT
MslI 1057 CACCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1339 CACTTCGGTG
PleI 667 GACTC GAGTC(4/5)
733 GAGTC
RsaI 189 GTAC GT^AC
1590 GTAC
SfaNI 118 GCATC GCATC(5/9)
437 GCATC
TaqI 1040 TCGA T^CGA
1160 TCGA
Tsp45I 1337 GTCAC ^GT[GC]AC
1529 GTGAC
TthHB8I 1040 TCGA T^CGA
1160 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
ApaI AvaI AvaII BanI BanII
BbeI BbvI BcnI BglI BmyI
BsaHI BsaJI BsaWI BsiEI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BssHII BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI KspI
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NgoMIV NlaIII NlaIV PstI PvuII
SacII Sau96I ScrFI SfcI SfoI
SgrAI SmaI StyI TseI TspRI
XmaCI XmaI