Restriction map for
pFN21AE1173
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AvrII BalI BanI
BbeI BbrPI BbvCI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FauI FbaI FseI Hin1I HpaI
KasI KpnI MfeI MluI MluNI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PstI
PvuI RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SnaBI Sse8387I SspBI
SspI SwaI Tsp45I Tth111I XbaI
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
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AccII 32 CGCG CG^CG
AccIII 893 TCCGGA T^CCGGA
AfaI 1165 GTAC GT^AC
AlwNI 1546 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 356 AGCGCT AGC^GCT
AseI 1192 ATTAAT AT^TAAT
AsnI 1192 ATTAAT AT^TAAT
AvaI 1252 CCCGGG C^[CT]CG[GA]G
AvaII 1144 GGTCC G^G[AT]CC
AviII 1484 TGCGCA TGC^GCA
BamHI 711 GGATCC G^GATCC
BbsI 566 GTCTTC GAAGAC(2/6)
BcgI 315 GCAGCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 566 GTCTTC GAAGAC(2/6)
BsaBI 1258 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 893 TCCGGA [AT]^CCGG[AT]
BseAI 893 TCCGGA T^CCGGA
BsmI 956 GAATGC GAATGC(1/-1)
BsoBI 1252 CCCGGG C^[CT]CG[GA]G
BspEI 893 TCCGGA T^CCGGA
BspLU11I 887 ACATGT A^CATGT
BsrDI 785 CATTGC GCAATG(2/0)
BstUI 32 CGCG CG^CG
BstXI 1587 CCAGTTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 1143 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 31 CCGCGG C^C[GA][CT]GG
EaeI 1103 CGGCCA [CT]^GGCC[GA]
EarI 1420 CTCTTC CTCTTC(1/4)
Eco47III 356 AGCGCT AGC^GCT
EcoO109I 1143 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 148 GATATC GAT^ATC
EcoT14I 1711 CCAAGG C^C[AT][AT]GG
FspI 1484 TGCGCA TGC^GCA
HaeII 356 AGCGCT [GA]GCGC^[CT]
HincII 883 GTTGAC GT[CT]^[GA]AC
HindII 883 GTTGAC GT[CT]^[GA]AC
HindIII 1388 AAGCTT A^AGCTT
Ksp632I 1420 CTCTTC CTCTTC(1/4)
KspI 31 CCGCGG CCGC^GG
MamI 1258 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 893 TCCGGA T^CCGGA
MvnI 32 CGCG CG^CG
PpuMI 1143 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 812 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1192 ATTAAT AT^TAAT
PvuII 968 CAGCTG CAG^CTG
RsaI 1165 GTAC GT^AC
SacII 31 CCGCGG CCGC^GG
SfcI 1373 CTACAG C^T[GA][CT]AG
SmaI 1252 CCCGGG CCC^GGG
SmlI 491 CTCAAG C^T[CT][GA]AG
SpeI 1353 ACTAGT A^CTAGT
SphI 1481 GCATGC GCATG^C
StuI 1491 AGGCCT AGG^CCT
StyI 1711 CCAAGG C^C[AT][AT]GG
XmaCI 1252 CCCGGG C^CCGGG
XmaI 1252 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 887 ACATGT A^C[GA][CT]GT
1787 ACGTGT
Asp700I 1513 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1603 GAATTTCTTC
BanII 11 GAGCCC G[GA]GC[CT]^C
779 GAGCCC
BfaI 983 CTAG C^TAG
1354 CTAG
BmyI 11 GAGCCC G[AGT]GC[ACT]^C
779 GAGCCC
BseRI 1618 CTCCTC GAGGAG(10/8)
8 GAGGAG
BsmAI 819 GTCTC GTCTC(1/5)
1465 GTCTC
BsmFI 810 GGGAC GGGAC(10/14)
1224 GTCCC
Bsp1286I 11 GAGCCC G[AGT]GC[ACT]^C
779 GAGCCC
BstBI 1520 TTCGAA TT^CGAA
1781 TTCGAA
CfoI 357 GCGC GCG^C
1485 GCGC
Cfr13I 45 GGCCC G^G[ACGT]CC
1144 GGTCC
HgaI 829 GACGC GACGC(5/10)
1054 GCGTC
HhaI 357 GCGC GCG^C
1485 GCGC
HinP1I 357 GCGC G^CGC
1485 GCGC
HphI 639 TCACC GGTGA(8/7)
1271 TCACC
MaeI 983 CTAG C^TAG
1354 CTAG
MaeII 1563 ACGT A^CGT
1787 ACGT
MspA1I 31 CCGCGG C[AC]G^C[GT]G
968 CAGCTG
NspI 887 ACATGT [GA]CATG^[CT]
1481 GCATGC
NspV 1520 TTCGAA TT^CGAA
1781 TTCGAA
PflMI 238 CCACAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
296 CCAAACTCTGG
PleI 1554 GACTC GAGTC(4/5)
36 GAGTC
Sau96I 45 GGCCC G^G[ACGT]CC
1144 GGTCC
SfuI 1520 TTCGAA TT^CGAA
1781 TTCGAA
Van91I 238 CCACAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
296 CCAAACTCTGG
XmnI 1513 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1603 GAATTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbvI BcnI BpmI BsaJI BsiYI
BslI BsrI BssKI BstF5I BstNI
BstYI Cac8I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI ScrFI SfaNI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII