Restriction map for
pFN21AE3125
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccII AccIII AcsI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BciVI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsiYI
BslI BsmBI Bsp1407I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MslI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
PvuII RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 428 GTAGAC GT^[AC][GT]AC
AclI 317 AACGTT AA^CGTT
AflIII 407 ACATGT A^C[GA][CT]GT
AlwNI 109 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 706 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 740 CCCGGG C^[CT]CG[GA]G
AvaII 370 GGACC G^G[AT]CC
BanII 329 GAGCTC G[GA]GC[CT]^C
BbsI 589 GAAGAC GAAGAC(2/6)
BcgI 175 GCATGGTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 589 GAAGAC GAAGAC(2/6)
BsaBI 661 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 73 GAGGAG GAGGAG(10/8)
BsgI 281 GTGCAG GTGCAG(16/14)
BsmFI 521 GTCCC GGGAC(10/14)
BsmI 710 GCATTC GAATGC(1/-1)
BsoBI 740 CCCGGG C^[CT]CG[GA]G
BspLU11I 407 ACATGT A^CATGT
BstBI 446 TTCGAA TT^CGAA
EcoRV 850 GATATC GAT^ATC
EcoT14I 611 CCTTGG C^C[AT][AT]GG
HapII 741 CCGG C^CGG
HpaII 741 CCGG C^CGG
MaeII 318 ACGT A^CGT
MamI 661 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 424 TTAA T^TAA
MspI 741 CCGG C^CGG
NspI 407 ACATGT [GA]CATG^[CT]
NspV 446 TTCGAA TT^CGAA
Psp1406I 317 AACGTT AA^CGTT
SacI 329 GAGCTC GAGCT^C
SapI 237 GAAGAGC GCTCTTC(1/4)
ScaI 22 AGTACT AGT^ACT
SfuI 446 TTCGAA TT^CGAA
SmaI 740 CCCGGG CCC^GGG
StyI 611 CCTTGG C^C[AT][AT]GG
Tru9I 424 TTAA T^TAA
XmaCI 740 CCCGGG C^CCGGG
XmaI 740 CCCGGG C^CCGGG
XmnI 706 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AciI 761 CCGC CCGC(-3/-1)
127 GCGG
AlwI 153 GATCC GGATC(4/5)
491 GGATC
AspHI 180 GTGCTC G[AT]GC[AT]^C
329 GAGCTC
BalI 158 TGGCCA TGG^CCA
830 TGGCCA
BbvI 229 GCAGC GCAGC(8/12)
48 GCTGC
BcnI 740 CCCGG CC^[GC]GG
741 CCGGG
BfaI 70 CTAG C^TAG
563 CTAG
BmyI 180 GTGCTC G[AGT]GC[ACT]^C
329 GAGCTC
BsiHKAI 180 GTGCTC G[AT]GC[AT]^C
329 GAGCTC
Bsp1286I 180 GTGCTC G[AGT]GC[ACT]^C
329 GAGCTC
BspDI 121 ATCGAT AT^CGAT
385 ATCGAT
BstYI 152 AGATCC [GA]^GATC[CT]
491 GGATCT
Cac8I 763 GCACGC GC[ACGT]^[ACGT]GC
767 GCTTGC
Cfr13I 370 GGACC G^G[ACGT]CC
738 GGCCC
ClaI 121 ATCGAT AT^CGAT
385 ATCGAT
EaeI 158 TGGCCA [CT]^GGCC[GA]
830 TGGCCA
EarI 237 GAAGAG CTCTTC(1/4)
532 GAAGAG
Fnu4HI 48 GCTGC GC^[ACGT]GC
229 GCAGC
ItaI 48 GCTGC GC^[ACGT]GC
229 GCAGC
Ksp632I 237 GAAGAG CTCTTC(1/4)
532 GAAGAG
MaeI 70 CTAG C^TAG
563 CTAG
MflI 152 AGATCC [GA]^GATC[CT]
491 GGATCT
MluNI 158 TGGCCA TGG^CCA
830 TGGCCA
MscI 158 TGGCCA TGG^CCA
830 TGGCCA
MwoI 48 GCTGCAGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
274 GCCAATCGTGC
NciI 740 CCCGG CC^[GC]GG
741 CCGGG
PstI 49 CTGCAG CTGCA^G
115 CTGCAG
Sau96I 370 GGACC G^G[ACGT]CC
738 GGCCC
SfaNI 124 GATGC GCATC(5/9)
65 GCATC
SfcI 49 CTGCAG C^T[GA][CT]AG
115 CTGCAG
TfiI 444 GATTC G^A[AT]TC
506 GATTC
TseI 48 GCTGC G^C[AT]GC
229 GCAGC
Tsp45I 39 GTCAC ^GT[GC]AC
724 GTGAC
Tsp509I 11 AATT ^AATT
422 AATT
XhoII 152 AGATCC [GA]^GATC[CT]
491 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AfaI AluI BsaJI BsmAI BsrI
BssKI BstF5I BstNI DdeI DpnII
EcoRII FokI HaeIII HinfI HphI
MaeIII MboI MboII MnlI MvaI
NdeII NlaIII PleI RsaI Sau3AI
ScrFI TaqI TspRI TthHB8I