Restriction map for
pFN21AE5917
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
AluI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BbeI BbrPI BbsI BbvCI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HapII HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PstI PvuII RsrII
SacI SacII SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI Tsp509I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 202 GTCGAC GT^[AC][GT]AC
AflIII 445 ACGTGT A^C[GA][CT]GT
AlwNI 235 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 54 GGGCCC GGGCC^C
AspHI 905 GAGCAC G[AT]GC[AT]^C
AvaII 468 GGTCC G^G[AT]CC
BalI 368 TGGCCA TGG^CCA
BamHI 677 GGATCC G^GATCC
BcgI 245 CGAACCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 364 TACGTG [CT]AC^GT[GA]
BsiEI 357 CGATCG CG[GA][CT]^CG
BsiHKAI 905 GAGCAC G[AT]GC[AT]^C
Bsp1407I 770 TGTACA T^GTACA
BspMI 715 ACCTGC ACCTGC(4/8)
BsrGI 770 TGTACA T^GTACA
BstYI 677 GGATCC [GA]^GATC[CT]
EcoT14I 58 CCAAGG C^C[AT][AT]GG
FauI 788 CCCGC CCCGC(4/6)
HgaI 291 GACGC GACGC(5/10)
HincII 202 GTCGAC GT[CT]^[GA]AC
HindII 202 GTCGAC GT[CT]^[GA]AC
MflI 677 GGATCC [GA]^GATC[CT]
MluNI 368 TGGCCA TGG^CCA
MscI 368 TGGCCA TGG^CCA
MseI 851 TTAA T^TAA
MwoI 231 GCCCCAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 234 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 15 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 357 CGATCG CGAT^CG
SalI 202 GTCGAC G^TCGAC
SapI 84 GCTCTTC GCTCTTC(1/4)
SexAI 581 ACCAGGT A^CC[AT]GGT
SspBI 770 TGTACA T^GTACA
StuI 704 AGGCCT AGG^CCT
StyI 58 CCAAGG C^C[AT][AT]GG
TaqI 203 TCGA T^CGA
TfiI 63 GATTC G^A[AT]TC
Tru9I 851 TTAA T^TAA
TthHB8I 203 TCGA T^CGA
Van91I 234 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 677 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 174 GTAC GT^AC
771 GTAC
AlwI 678 GATCC GGATC(4/5)
677 GGATC
BanI 228 GGTGCC G^G[CT][GA]CC
892 GGTGCC
BpmI 153 CTCCAG CTGGAG(16/14)
408 CTGGAG
BsmAI 332 GTCTC GTCTC(1/5)
643 GTCTC
BsmFI 741 GGGAC GGGAC(10/14)
786 GTCCC
BspHI 401 TCATGA T^CATGA
510 TCATGA
DpnII 358 GATC ^GATC
678 GATC
EarI 85 CTCTTC CTCTTC(1/4)
745 CTCTTC
HphI 107 TCACC GGTGA(8/7)
422 TCACC
Ksp632I 85 CTCTTC CTCTTC(1/4)
745 CTCTTC
MaeII 365 ACGT A^CGT
445 ACGT
MboI 358 GATC ^GATC
678 GATC
NdeII 358 GATC ^GATC
678 GATC
RcaI 401 TCATGA T^CATGA
510 TCATGA
RsaI 174 GTAC GT^AC
771 GTAC
Sau3AI 358 GATC ^GATC
678 GATC
Tsp45I 22 GTCAC ^GT[GC]AC
433 GTCAC
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AocI BanII BbvI BmyI
BsaJI BseRI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Bsu36I
Cac8I Cfr13I DdeI EaeI Eco81I
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MaeIII MboII MnlI MslI
MvaI NlaIII NlaIV PleI Sau96I
ScrFI SfaNI TseI TspRI