Restriction map for
pFN21AE5916
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI AlwNI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MluI MroI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StyI SwaI
Tth111I Van91I XcmI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AflIII 206 ACATGT A^C[GA][CT]GT
Alw44I 417 GTGCAC G^TGCAC
AocI 870 CCTTAGG CC^T[ACGT]AGG
ApaLI 417 GTGCAC G^TGCAC
AspHI 417 GTGCAC G[AT]GC[AT]^C
AvaI 219 CTCGAG C^[CT]CG[GA]G
BamHI 677 GGATCC G^GATCC
BbsI 178 GTCTTC GAAGAC(2/6)
BpuAI 178 GTCTTC GAAGAC(2/6)
BsaI 642 GGTCTC GGTCTC(1/5)
BsaJI 132 CCTGGG C^C[ACGT][ACGT]GG
BseRI 720 CTCCTC GAGGAG(10/8)
BsgI 160 CTGCAC GTGCAG(16/14)
BsiHKAI 417 GTGCAC G[AT]GC[AT]^C
BsmAI 643 GTCTC GTCTC(1/5)
BsmFI 229 GTCCC GGGAC(10/14)
BsmI 876 GAATGC GAATGC(1/-1)
BsoBI 219 CTCGAG C^[CT]CG[GA]G
Bsp1407I 770 TGTACA T^GTACA
BspLU11I 206 ACATGT A^CATGT
BspMI 715 ACCTGC ACCTGC(4/8)
BsrBI 625 CCGCTC CCGCTC(-3/-3)
BsrGI 770 TGTACA T^GTACA
Bsu36I 870 CCTTAGG CC^T[ACGT]AGG
Cac8I 240 GCTAGC GC[ACGT]^[ACGT]GC
CfoI 758 GCGC GCG^C
Eco81I 870 CCTTAGG CC^T[ACGT]AGG
FauI 598 GCGGG CCCGC(4/6)
HaeII 757 AGCGCC [GA]GCGC^[CT]
HhaI 758 GCGC GCG^C
HinP1I 758 GCGC G^CGC
MseI 106 TTAA T^TAA
MwoI 598 GCGGGTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NheI 240 GCTAGC G^CTAGC
NspI 206 ACATGT [GA]CATG^[CT]
PaeR7I 219 CTCGAG C^TCGAG
ScaI 223 AGTACT AGT^ACT
SexAI 581 ACCAGGT A^CC[AT]GGT
SspBI 770 TGTACA T^GTACA
StuI 704 AGGCCT AGG^CCT
TaqI 220 TCGA T^CGA
TfiI 44 GATTC G^A[AT]TC
Tru9I 106 TTAA T^TAA
Tsp509I 554 AATT ^AATT
TspRI 420 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 220 TCGA T^CGA
XbaI 154 TCTAGA T^CTAGA
XhoI 219 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AfaI 224 GTAC GT^AC
771 GTAC
BalI 194 TGGCCA TGG^CCA
665 TGGCCA
BanII 136 GGGCTC G[GA]GC[CT]^C
893 GGGCTC
BbvI 755 GCAGC GCAGC(8/12)
492 GCTGC
BpmI 64 CTGGAG CTGGAG(16/14)
408 CTGGAG
BsrI 407 ACTGG ACTGG(1/-1)
681 CCAGT
BstF5I 33 CATCC GGATG(2/0)
657 CATCC
BstYI 677 GGATCC [GA]^GATC[CT]
812 AGATCC
EaeI 194 TGGCCA [CT]^GGCC[GA]
665 TGGCCA
FokI 33 CATCC GGATG(9/13)
657 CATCC
MaeIII 186 GTCAC ^GT[ACGT]AC
377 GTCAC
MboII 810 GAAGA GAAGA(8/7)
179 TCTTC
MflI 677 GGATCC [GA]^GATC[CT]
812 AGATCC
MluNI 194 TGGCCA TGG^CCA
665 TGGCCA
MscI 194 TGGCCA TGG^CCA
665 TGGCCA
PleI 151 GACTC GAGTC(4/5)
905 GAGTC
RsaI 224 GTAC GT^AC
771 GTAC
SfaNI 237 GATGC GCATC(5/9)
255 GATGC
SfcI 99 CTACAG C^T[GA][CT]AG
864 CTACAG
TseI 492 GCTGC G^C[AT]GC
755 GCAGC
Tsp45I 186 GTCAC ^GT[GC]AC
377 GTCAC
XhoII 677 GGATCC [GA]^GATC[CT]
812 AGATCC
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI AlwI AvaII BfaI
BmyI BsiYI BslI Bsp1286I BssKI
BstNI Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HinfI HphI ItaI
MaeI MboI MnlI MslI MvaI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SmlI