Restriction map for
pFN21AE5914
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AluI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HindIII
HpaI HpaII KasI KpnI KspI
MamI MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp45I
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 202 GTCGAC GT^[AC][GT]AC
AlwI 678 GATCC GGATC(4/5)
AseI 577 ATTAAT AT^TAAT
AsnI 577 ATTAAT AT^TAAT
Asp700I 702 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 505 GGACC G^G[AT]CC
BalI 368 TGGCCA TGG^CCA
BciVI 567 GGATAC GTATCC(6/5)
BfaI 475 CTAG C^TAG
BpmI 153 CTCCAG CTGGAG(16/14)
BsaAI 364 TACGTG [CT]AC^GT[GA]
BsiEI 197 CGGTCG CG[GA][CT]^CG
BsmAI 332 GTCTC GTCTC(1/5)
BsmFI 124 GGGAC GGGAC(10/14)
BspHI 401 TCATGA T^CATGA
BstYI 677 AGATCC [GA]^GATC[CT]
DdeI 871 CTTAG C^T[ACGT]AG
EaeI 368 TGGCCA [CT]^GGCC[GA]
HincII 202 GTCGAC GT[CT]^[GA]AC
HindII 202 GTCGAC GT[CT]^[GA]AC
MaeI 475 CTAG C^TAG
MaeII 365 ACGT A^CGT
MaeIII 480 GTTAC ^GT[ACGT]AC
MflI 677 AGATCC [GA]^GATC[CT]
MluNI 368 TGGCCA TGG^CCA
MscI 368 TGGCCA TGG^CCA
MwoI 231 GCCCCAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 234 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 577 ATTAAT AT^TAAT
RcaI 401 TCATGA T^CATGA
SalI 202 GTCGAC G^TCGAC
SapI 84 GCTCTTC GCTCTTC(1/4)
TaqI 203 TCGA T^CGA
TfiI 63 GATTC G^A[AT]TC
TthHB8I 203 TCGA T^CGA
Van91I 234 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 677 AGATCC [GA]^GATC[CT]
XmnI 702 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AciI 196 GCGG CCGC(-3/-1)
565 GCGG
AlwNI 235 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
605 CAGTGTCTG
BanI 228 GGTGCC G^G[CT][GA]CC
695 GGTGCC
BbvI 221 GCAGC GCAGC(8/12)
284 GCTGC
BstF5I 256 CATCC GGATG(2/0)
905 GGATG
Cac8I 217 GCTTGC GC[ACGT]^[ACGT]GC
280 GCTGGC
DpnII 358 GATC ^GATC
678 GATC
Fnu4HI 221 GCAGC GC^[ACGT]GC
284 GCTGC
FokI 256 CATCC GGATG(9/13)
905 GGATG
HphI 243 GGTGA GGTGA(8/7)
345 GGTGA
ItaI 221 GCAGC GC^[ACGT]GC
284 GCTGC
MboI 358 GATC ^GATC
678 GATC
NdeII 358 GATC ^GATC
678 GATC
Sau3AI 358 GATC ^GATC
678 GATC
TseI 221 GCAGC G^C[AT]GC
284 GCTGC
Tsp509I 537 AATT ^AATT
861 AATT
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AfaI BanII BmyI BsaJI BseRI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI Cfr13I EarI EcoRII EcoT14I
HaeIII HinfI Ksp632I MboII MnlI
MseI MslI MvaI NlaIII NlaIV
PleI RsaI Sau96I ScrFI SfaNI
StyI Tru9I TspRI