Restriction map for
pFN21AE5358
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BcnI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeI MaeII MaeIII MamI
MfeI MflI MluI MroI MslI
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI TfiI
Tsp45I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 814 GTCTAC GT^[AC][GT]AC
AfaI 225 GTAC GT^AC
Alw44I 190 GTGCAC G^TGCAC
AlwI 138 GATCC GGATC(4/5)
AlwNI 886 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 190 GTGCAC G^TGCAC
AseI 610 ATTAAT AT^TAAT
AsnI 610 ATTAAT AT^TAAT
AvaII 666 GGACC G^G[AT]CC
BalI 796 TGGCCA TGG^CCA
BbsI 50 GTCTTC GAAGAC(2/6)
BclI 749 TGATCA T^GATCA
BpuAI 50 GTCTTC GAAGAC(2/6)
BseRI 621 GAGGAG GAGGAG(10/8)
BsiEI 240 CGGCCG CG[GA][CT]^CG
BsmI 638 GAATGC GAATGC(1/-1)
BspMI 116 GCAGGT ACCTGC(4/8)
BstAPI 654 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 869 TTCGAA TT^CGAA
Cfr13I 666 GGACC G^G[ACGT]CC
DraII 665 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 799 CCATGG C^C[GA][CT]GG
EagI 240 CGGCCG C^GGCCG
EclXI 240 CGGCCG C^GGCCG
Eco52I 240 CGGCCG C^GGCCG
EcoO109I 665 AGGACCC [GA]G^G[ACGT]CC[CT]
FauI 161 CCCGC CCCGC(4/6)
FbaI 749 TGATCA T^GATCA
HindIII 865 AAGCTT A^AGCTT
HinfI 48 GAGTC G^A[ACGT]TC
HphI 450 GGTGA GGTGA(8/7)
MluNI 796 TGGCCA TGG^CCA
MscI 796 TGGCCA TGG^CCA
MspA1I 300 CAGCTG C[AC]G^C[GT]G
NcoI 799 CCATGG C^CATGG
NspV 869 TTCGAA TT^CGAA
PacI 607 TTAATTAA TTAAT^TAA
PflMI 284 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 48 GAGTC GAGTC(4/5)
PpuMI 665 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 610 ATTAAT AT^TAAT
PvuII 300 CAGCTG CAG^CTG
RsaI 225 GTAC GT^AC
SacI 692 GAGCTC GAGCT^C
Sau96I 666 GGACC G^G[ACGT]CC
SbfI 660 CCTGCAGG CCTGCA^GG
ScaI 224 AGTACT AGT^ACT
SfaNI 757 GATGC GCATC(5/9)
SfuI 869 TTCGAA TT^CGAA
SmlI 862 CTCAAG C^T[CT][GA]AG
Sse8387I 660 CCTGCAGG CCTGCA^GG
Van91I 284 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AciI 162 CCGC CCGC(-3/-1)
761 CCGC
Asp700I 69 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
485 GAAAATTTTC
AspHI 190 GTGCAC G[AT]GC[AT]^C
692 GAGCTC
BanI 154 GGTGCC G^G[CT][GA]CC
387 GGTGCC
BanII 405 GGGCTC G[GA]GC[CT]^C
692 GAGCTC
BsaI 235 GGTCTC GGTCTC(1/5)
591 GGTCTC
BsiHKAI 190 GTGCAC G[AT]GC[AT]^C
692 GAGCTC
BsmAI 236 GTCTC GTCTC(1/5)
592 GTCTC
EaeI 240 CGGCCG [CT]^GGCC[GA]
796 TGGCCA
EcoT14I 128 CCTTGG C^C[AT][AT]GG
799 CCATGG
PstI 172 CTGCAG CTGCA^G
661 CTGCAG
SfcI 172 CTGCAG C^T[GA][CT]AG
661 CTGCAG
StyI 128 CCTTGG C^C[AT][AT]GG
799 CCATGG
TaqI 221 TCGA T^CGA
870 TCGA
TthHB8I 221 TCGA T^CGA
870 TCGA
XmnI 69 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
485 GAAAATTTTC
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
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AcsI AluI ApoI BbvI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII ItaI MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI ScrFI Tru9I TseI
Tsp509I TspRI