Restriction map for
pFN21AE2191
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI AlwI
AlwNI AocI Aor51HI ApaI AscI
Asp700I Asp718I AspEI AspI AvaI
AviII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI BspDI BspEI BspLU11I BspMI
BsrDI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HindIII HpaI KasI KpnI
KspI MamI MflI MluI MluNI
MroI MscI MslI MspA1I MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I Van91I
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 652 GTAGAC GT^[AC][GT]AC
AcyI 3 GGCGTC G[GA]^CG[CT]C
Alw44I 779 GTGCAC G^TGCAC
ApaLI 779 GTGCAC G^TGCAC
AseI 144 ATTAAT AT^TAAT
AsnI 144 ATTAAT AT^TAAT
AvrII 826 CCTAGG C^CTAGG
BbvI 544 GCTGC GCAGC(8/12)
BclI 36 TGATCA T^GATCA
BcnI 641 CCGGG CC^[GC]GG
BlnI 826 CCTAGG C^CTAGG
BsaAI 454 TACGTG [CT]AC^GT[GA]
BsaHI 3 GGCGTC G[GA]^CG[CT]C
Bsp1407I 1062 TGTACA T^GTACA
BspHI 173 TCATGA T^CATGA
BsrBI 9 GAGCGG CCGCTC(-3/-3)
BsrGI 1062 TGTACA T^GTACA
BsrI 907 ACTGG ACTGG(1/-1)
BssSI 98 CTCGTG CACGAG(-5/-1)
BstNI 775 CCTGG CC^[AT]GG
Cac8I 769 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 849 GCGC GCG^C
DraII 645 GGGACCT [GA]G^G[ACGT]CC[CT]
EarI 860 GAAGAG CTCTTC(1/4)
EcoO109I 645 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 775 CCTGG ^CC[AT]GG
EcoT14I 826 CCTAGG C^C[AT][AT]GG
FauI 14 GCGGG CCCGC(4/6)
FbaI 36 TGATCA T^GATCA
HapII 641 CCGG C^CGG
HgaI 4 GCGTC GACGC(5/10)
HhaI 849 GCGC GCG^C
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HinP1I 849 GCGC G^CGC
HincII 811 GTTGAC GT[CT]^[GA]AC
HindII 811 GTTGAC GT[CT]^[GA]AC
HpaII 641 CCGG C^CGG
HphI 39 TCACC GGTGA(8/7)
Ksp632I 860 GAAGAG CTCTTC(1/4)
MfeI 212 CAATTG C^AATTG
MspI 641 CCGG C^CGG
MunI 212 CAATTG C^AATTG
MvaI 775 CCTGG CC^[AT]GG
NciI 641 CCGGG CC^[GC]GG
NdeI 419 CATATG CA^TATG
NlaIV 645 GGGACC GG[ACGT]^[ACGT]CC
PpuMI 645 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 144 ATTAAT AT^TAAT
RcaI 173 TCATGA T^CATGA
ScaI 137 AGTACT AGT^ACT
SspBI 1062 TGTACA T^GTACA
StyI 826 CCTAGG C^C[AT][AT]GG
TseI 544 GCTGC G^C[AT]GC
Tsp45I 457 GTGAC ^GT[GC]AC
TspRI 764 CAGTG CA[GC]TG[ACGT][ACGT]^
XbaI 231 TCTAGA T^CTAGA
XcmI 308 CCAAGAGAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AluI 20 AGCT AG^CT
543 AGCT
AspHI 659 GAGCAC G[AT]GC[AT]^C
779 GTGCAC
AvaII 646 GGACC G^G[AT]CC
928 GGACC
BbsI 250 GAAGAC GAAGAC(2/6)
798 GTCTTC
BmyI 659 GAGCAC G[AGT]GC[ACT]^C
779 GTGCAC
BpmI 365 CTCCAG CTGGAG(16/14)
1040 CTCCAG
BpuAI 250 GAAGAC GAAGAC(2/6)
798 GTCTTC
BseRI 618 CTCCTC GAGGAG(10/8)
669 CTCCTC
BsiHKAI 659 GAGCAC G[AT]GC[AT]^C
779 GTGCAC
BsiYI 48 CCAGAGGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
160 CCTGCTGTGGG
BslI 48 CCAGAGGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
160 CCTGCTGTGGG
BsmFI 87 GGGAC GGGAC(10/14)
645 GGGAC
Bsp1286I 659 GAGCAC G[AGT]GC[ACT]^C
779 GTGCAC
BssKI 641 CCGGG ^CC[ACGT]GG
775 CCTGG
DpnII 37 GATC ^GATC
871 GATC
Fnu4HI 11 GCGGC GC^[ACGT]GC
544 GCTGC
ItaI 11 GCGGC GC^[ACGT]GC
544 GCTGC
MboI 37 GATC ^GATC
871 GATC
MwoI 298 GCAAGGACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
781 GCACAAAAGGC
NdeII 37 GATC ^GATC
871 GATC
NlaIII 174 CATG CATG^
352 CATG
PstI 560 CTGCAG CTGCA^G
841 CTGCAG
Sau3AI 37 GATC ^GATC
871 GATC
ScrFI 641 CCGGG CC^[ACGT]GG
775 CCTGG
TaqI 7 TCGA T^CGA
81 TCGA
TfiI 130 GAATC G^A[AT]TC
194 GATTC
TthHB8I 7 TCGA T^CGA
81 TCGA
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AcsI AfaI ApoI BfaI
BsaJI BstF5I Cfr13I DdeI FokI
HaeIII HinfI MaeI MaeII MaeIII
MboII MnlI MseI PleI RsaI
Sau96I SfcI Tru9I Tsp509I