Restriction map for
pFN21AE5727
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# NO CUT ENZYMES ( 90 enzymes) #
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AclI AcsI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspHI AspI AvrII
BbsI BbvCI BclI BlnI BpuAI
BsaBI BsiHKAI BsiWI BsmI Bsp1407I
BspDI BspHI BsrGI BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I ClaI
DraI DsaI Eam1105I Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KspI
MamI MfeI MluI MunI NcoI
NdeI NheI NruI NsiI NspV
PacI PflFI PmeI PshAI PshBI
Psp1406I PvuI RcaI SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tth111I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 85 GGTACC G^GTACC
AccI 1390 GTCTAC GT^[AC][GT]AC
AccIII 1476 TCCGGA T^CCGGA
AflII 454 CTTAAG C^TTAAG
AflIII 1941 ACATGT A^C[GA][CT]GT
AgeI 75 ACCGGT A^CCGGT
Asp718I 85 GGTACC G^GTACC
BamHI 2031 GGATCC G^GATCC
BbrPI 1210 CACGTG CAC^GTG
BcgI 1590 CGAGATGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1473 GTATCC GTATCC(6/5)
BfaI 898 CTAG C^TAG
BfrI 454 CTTAAG C^TTAAG
BglII 275 AGATCT A^GATCT
BlpI 875 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 875 GCTCAGC GC^T[ACGT]AGC
BsaAI 1210 CACGTG [CT]AC^GT[GA]
BseAI 1476 TCCGGA T^CCGGA
BsmBI 296 GAGACG CGTCTC(1/5)
BspEI 1476 TCCGGA T^CCGGA
BspLU11I 1941 ACATGT A^CATGT
BsrDI 290 CATTGC GCAATG(2/0)
BsrI 649 CCAGT ACTGG(1/-1)
BstEII 1051 GGTCACC G^GT[ACGT]ACC
BstPI 1051 GGTCACC G^GT[ACGT]ACC
BstXI 724 CCAGGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 875 GCTCAGC GC^T[ACGT]AGC
CpoI 72 CGGACCG CG^G[AT]CCG
DraIII 475 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2053 GACGTGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1051 GGTCACC G^GT[ACGT]ACC
KpnI 85 GGTACC GGTAC^C
MaeI 898 CTAG C^TAG
MroI 1476 TCCGGA T^CCGGA
PaeR7I 1291 CTCGAG C^TCGAG
PflMI 630 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 75 ACCGGT A^CCGGT
PmaCI 1210 CACGTG CAC^GTG
PmlI 1210 CACGTG CAC^GTG
PstI 1505 CTGCAG CTGCA^G
RsrII 72 CGGACCG CG^G[AT]CCG
SfcI 1505 CTGCAG C^T[GA][CT]AG
Tsp509I 1448 AATT ^AATT
Van91I 630 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1291 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AatII 298 GACGTC GACGT^C
2059 GACGTC
Aor51HI 404 AGCGCT AGC^GCT
1295 AGCGCT
ApaI 639 GGGCCC GGGCC^C
1151 GGGCCC
AviII 146 TGCGCA TGC^GCA
1260 TGCGCA
BalI 1544 TGGCCA TGG^CCA
1962 TGGCCA
BglI 1645 GCCGTCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1955 GCCACCCTGGC
BsaI 565 GGTCTC GGTCTC(1/5)
1327 GGTCTC
BsaWI 75 ACCGGT [AT]^CCGG[AT]
1476 TCCGGA
BssHII 1218 GCGCGC G^CGCGC
1301 GCGCGC
EarI 693 CTCTTC CTCTTC(1/4)
1189 GAAGAG
Eco47III 404 AGCGCT AGC^GCT
1295 AGCGCT
EcoT14I 341 CCTTGG C^C[AT][AT]GG
534 CCAAGG
FspI 146 TGCGCA TGC^GCA
1260 TGCGCA
Ksp632I 693 CTCTTC CTCTTC(1/4)
1189 GAAGAG
MaeIII 1052 GTCAC ^GT[ACGT]AC
2051 GTGAC
MluNI 1544 TGGCCA TGG^CCA
1962 TGGCCA
MscI 1544 TGGCCA TGG^CCA
1962 TGGCCA
MseI 208 TTAA T^TAA
455 TTAA
MslI 27 CACCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1799 CACTCAAGTG
NotI 913 GCGGCCGC GC^GGCCGC
1510 GCGGCCGC
NspI 1772 ACATGC [GA]CATG^[CT]
1941 ACATGT
PpuMI 1109 GGGACCC [GA]G^G[AT]CC[CT]
1928 GGGACCT
StuI 656 AGGCCT AGG^CCT
689 AGGCCT
StyI 341 CCTTGG C^C[AT][AT]GG
534 CCAAGG
Tru9I 208 TTAA T^TAA
455 TTAA
Tsp45I 1052 GTCAC ^GT[GC]AC
2051 GTGAC
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# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BanI
BanII BbeI BbvI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiEI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EagI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfoI SmaI SmlI
TaqI TseI TspRI TthHB8I XhoII
XmaCI XmaI