Restriction map for
pFN21AE3472
##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI Asp718I
AspEI AvrII BfrI BlnI BsaI
BsaWI BseAI BsiWI Bsp1407I BspEI
BspLU11I BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I CpoI DraIII Eam1105I
Eco47III EcoO65I EcoRI EcoT22I FseI
KpnI MluI MroI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI Psp1406I PvuI RsrII
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflIII 3937 ACGTGT A^C[GA][CT]GT
AlwNI 3090 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1778 GGGCCC GGGCC^C
AseI 2931 ATTAAT AT^TAAT
AsnI 2931 ATTAAT AT^TAAT
Asp700I 3038 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 185 TGCGCA TGC^GCA
BalI 2792 TGGCCA TGG^CCA
BamHI 2179 GGATCC G^GATCC
BbvCI 3616 CCTCAGC CCTCAGC(-5/-2)
BcgI 3979 GCAGGGGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3663 GGATAC GTATCC(6/5)
BclI 551 TGATCA T^GATCA
BsaBI 3508 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 4347 GAATGC GAATGC(1/-1)
BspDI 4413 ATCGAT AT^CGAT
BspHI 1491 TCATGA T^CATGA
BspMI 3624 GCAGGT ACCTGC(4/8)
BsrDI 3580 GCAATG GCAATG(2/0)
BssHII 104 GCGCGC G^CGCGC
BssSI 1507 CACGAG CACGAG(-5/-1)
BstAPI 4399 GCAACTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 4413 ATCGAT AT^CGAT
EagI 419 CGGCCG C^GGCCG
EclXI 419 CGGCCG C^GGCCG
Eco52I 419 CGGCCG C^GGCCG
EcoRV 3643 GATATC GAT^ATC
FbaI 551 TGATCA T^GATCA
FspI 185 TGCGCA TGC^GCA
HindIII 3560 AAGCTT A^AGCTT
HpaI 922 GTTAAC GTT^AAC
MamI 3508 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2792 TGGCCA TGG^CCA
MscI 2792 TGGCCA TGG^CCA
NaeI 252 GCCGGC GCC^GGC
NgoMIV 252 GCCGGC G^CCGGC
PaeR7I 405 CTCGAG C^TCGAG
PshAI 3822 GACTTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2931 ATTAAT AT^TAAT
RcaI 1491 TCATGA T^CATGA
SacI 3379 GAGCTC GAGCT^C
StuI 3747 AGGCCT AGG^CCT
XbaI 1720 TCTAGA T^CTAGA
XhoI 405 CTCGAG C^TCGAG
XmnI 3038 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1939 GTCTAC GT^[AC][GT]AC
2050 GTAGAC
Alw44I 1046 GTGCAC G^TGCAC
2709 GTGCAC
ApaLI 1046 GTGCAC G^TGCAC
2709 GTGCAC
AspI 743 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1933 GACCCTGTC
BbrPI 1049 CACGTG CAC^GTG
3936 CACGTG
BglII 1474 AGATCT A^GATCT
1486 AGATCT
BlpI 1669 GCTGAGC GC^T[ACGT]AGC
2350 GCTGAGC
Bpu1102I 1669 GCTGAGC GC^T[ACGT]AGC
2350 GCTGAGC
BsaAI 1049 CACGTG [CT]AC^GT[GA]
3936 CACGTG
BsiEI 419 CGGCCG CG[GA][CT]^CG
480 CGGTCG
BsmBI 458 CGTCTC CGTCTC(1/5)
3590 CGTCTC
BsrBI 229 CCGCTC CCGCTC(-3/-3)
157 GAGCGG
BstXI 1117 CCAATGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2603 CCATCCAGTTGG
CelII 1669 GCTGAGC GC^T[ACGT]AGC
2350 GCTGAGC
DraI 1137 TTTAAA TTT^AAA
1962 TTTAAA
DrdI 264 GACTTCCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2882 GACCCCACAGTC
EcoNI 64 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3193 CCTACCCCAGG
PflFI 743 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1933 GACCCTGTC
PflMI 315 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2424 CCACTCAATGG
PmaCI 1049 CACGTG CAC^GTG
3936 CACGTG
PmlI 1049 CACGTG CAC^GTG
3936 CACGTG
PpuMI 2808 AGGACCT [GA]G^G[AT]CC[CT]
3398 AGGACCT
ScaI 1273 AGTACT AGT^ACT
2332 AGTACT
SmaI 42 CCCGGG CCC^GGG
427 CCCGGG
SphI 1346 GCATGC GCATG^C
1900 GCATGC
Tsp45I 3323 GTGAC ^GT[GC]AC
3820 GTGAC
Tth111I 743 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1933 GACCCTGTC
Van91I 315 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2424 CCACTCAATGG
XmaCI 42 CCCGGG C^CCGGG
427 CCCGGG
XmaI 42 CCCGGG C^CCGGG
427 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AocI ApoI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MspA1I MspI MunI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SacII SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XcmI
XhoII