Restriction map for
pFN21AE5721
##################################
# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI Asp700I
Asp718I AspEI AspHI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BciVI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspHI BspLU11I BsrFI BssHII
BssSI BstUI BstXI Bsu36I CelII
CfoI Cfr10I CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SmaI SmlI SpeI
Sse8387I StuI SwaI Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AclI 1402 AACGTT AA^CGTT
ApaI 2053 GGGCCC GGGCC^C
AseI 1009 ATTAAT AT^TAAT
AsnI 1009 ATTAAT AT^TAAT
AspI 622 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 480 CTCGGG C^[CT]CG[GA]G
BanI 253 GGTGCC G^G[CT][GA]CC
BanII 2053 GGGCCC G[GA]GC[CT]^C
BcgI 1622 GCAGCTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1863 AGATCT A^GATCT
BmyI 2053 GGGCCC G[AGT]GC[ACT]^C
BsmAI 1724 GTCTC GTCTC(1/5)
BsmFI 2034 GGGAC GGGAC(10/14)
BsoBI 480 CTCGGG C^[CT]CG[GA]G
Bsp1286I 2053 GGGCCC G[AGT]GC[ACT]^C
BspDI 637 ATCGAT AT^CGAT
BspMI 245 ACCTGC ACCTGC(4/8)
BsrBI 2042 GAGCGG CCGCTC(-3/-3)
Bst1107I 1048 GTATAC GTA^TAC
BstBI 1268 TTCGAA TT^CGAA
BstEII 664 GGTTACC G^GT[ACGT]ACC
BstPI 664 GGTTACC G^GT[ACGT]ACC
BstZ17I 1048 GTATAC GTA^TAC
ClaI 637 ATCGAT AT^CGAT
DraI 736 TTTAAA TTT^AAA
DraII 2034 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 2034 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 664 GGTTACC G^GT[ACGT]ACC
EcoRV 1531 GATATC GAT^ATC
EcoT14I 1979 CCAAGG C^C[AT][AT]GG
MslI 934 CACTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspV 1268 TTCGAA TT^CGAA
PflFI 622 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2034 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 1009 ATTAAT AT^TAAT
Psp1406I 1402 AACGTT AA^CGTT
SapI 336 GAAGAGC GCTCTTC(1/4)
SexAI 1915 ACCAGGT A^CC[AT]GGT
SfcI 1497 CTACAG C^T[GA][CT]AG
SfuI 1268 TTCGAA TT^CGAA
SphI 112 GCATGC GCATG^C
StyI 1979 CCAAGG C^C[AT][AT]GG
Tth111I 622 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1772 CCAAAACTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 2002 CCGC CCGC(-3/-1)
2044 GCGG
AlwNI 241 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
1957 CAGAAACTG
BpmI 1505 CTGGAG CTGGAG(16/14)
2039 CTGGAG
BsaAI 613 TACGTA [CT]AC^GT[GA]
1045 TACGTA
BsaJI 479 CCTCGG C^C[ACGT][ACGT]GG
1979 CCAAGG
BseRI 421 GAGGAG GAGGAG(10/8)
448 GAGGAG
BsiYI 1018 CCTGAATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1926 CCGAATCTTGG
BslI 1018 CCTGAATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1926 CCGAATCTTGG
Bsp1407I 530 TGTACA T^GTACA
1547 TGTACA
BsrDI 258 CATTGC GCAATG(2/0)
1836 CATTGC
BsrGI 530 TGTACA T^GTACA
1547 TGTACA
BstF5I 98 GGATG GGATG(2/0)
132 GGATG
BstYI 1827 GGATCT [GA]^GATC[CT]
1863 AGATCT
EaeI 1026 TGGCCG [CT]^GGCC[GA]
1685 CGGCCA
EcoT22I 941 ATGCAT ATGCA^T
1261 ATGCAT
FokI 98 GGATG GGATG(9/13)
132 GGATG
HphI 1090 GGTGA GGTGA(8/7)
1633 GGTGA
MflI 1827 GGATCT [GA]^GATC[CT]
1863 AGATCT
NsiI 941 ATGCAT ATGCA^T
1261 ATGCAT
SnaBI 613 TACGTA TAC^GTA
1045 TACGTA
SspBI 530 TGTACA T^GTACA
1547 TGTACA
XhoII 1827 GGATCT [GA]^GATC[CT]
1863 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BfaI BsmI
BsrI BssKI BstAPI BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI HaeIII HinfI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I