Restriction map for
pFN21AE6754
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccII AccIII AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbrPI BbvCI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BsrBI BssHII
BstBI BstUI CelII ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoRV EcoT22I FseI
FspI KpnI KspI MaeI MfeI
MluI MluNI MroI MscI MunI
MvnI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI RsrII SacII SalI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI SspI SwaI Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AclI 1141 AACGTT AA^CGTT
AfaI 834 GTAC GT^AC
AflIII 1772 ACATGT A^C[GA][CT]GT
AlwNI 547 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
BciVI 913 GTATCC GTATCC(6/5)
BclI 149 TGATCA T^GATCA
BglII 1553 AGATCT A^GATCT
BsaBI 150 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1946 TCCGGT [AT]^CCGG[AT]
Bsp1407I 833 TGTACA T^GTACA
BspLU11I 1772 ACATGT A^CATGT
BsrDI 1011 CATTGC GCAATG(2/0)
BsrGI 833 TGTACA T^GTACA
Bst1107I 1845 GTATAC GTA^TAC
BstAPI 2124 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1107 GGTGACC G^GT[ACGT]ACC
BstPI 1107 GGTGACC G^GT[ACGT]ACC
BstXI 1376 CCAAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1845 GTATAC GTA^TAC
DrdI 3 GACCCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1107 GGTGACC G^GT[ACGT]ACC
EcoRI 1369 GAATTC G^AATTC
FbaI 149 TGATCA T^GATCA
HindIII 1411 AAGCTT A^AGCTT
HpaI 1855 GTTAAC GTT^AAC
MamI 150 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 745 GCCGGC GCC^GGC
NcoI 1112 CCATGG C^CATGG
NdeI 1649 CATATG CA^TATG
NgoMIV 745 GCCGGC G^CCGGC
PaeR7I 872 CTCGAG C^TCGAG
PflMI 619 CCAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1141 AACGTT AA^CGTT
RsaI 834 GTAC GT^AC
SbfI 1976 CCTGCAGG CCTGCA^GG
SmaI 684 CCCGGG CCC^GGG
Sse8387I 1976 CCTGCAGG CCTGCA^GG
SspBI 833 TGTACA T^GTACA
StuI 1275 AGGCCT AGG^CCT
Van91I 619 CCAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 785 CCAGCATTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 872 CTCGAG C^TCGAG
XmaCI 684 CCCGGG C^CCGGG
XmaI 684 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccI 316 GTCTAC GT^[AC][GT]AC
1845 GTATAC
AcsI 476 GAATTT [GA]^AATT[CT]
1369 GAATTC
ApoI 476 GAATTT [GA]^AATT[CT]
1369 GAATTC
BbeI 859 GGCGCC GGCGC^C
2016 GGCGCC
BsaI 1788 GGTCTC GGTCTC(1/5)
1958 GGTCTC
BsmAI 1789 GTCTC GTCTC(1/5)
1959 GTCTC
BsmI 2067 GAATGC GAATGC(1/-1)
788 GCATTC
BssSI 822 CTCGTG CACGAG(-5/-1)
2110 CTCGTG
BstYI 1553 AGATCT [GA]^GATC[CT]
1750 AGATCC
DsaI 1079 CCGTGG C^C[GA][CT]GG
1112 CCATGG
EcoNI 1973 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2025 CCTGGCTAAGG
FauI 995 CCCGC CCCGC(4/6)
1570 CCCGC
HaeII 859 GGCGCC [GA]GCGC^[CT]
2016 GGCGCC
HgaI 281 GCGTC GACGC(5/10)
314 GCGTC
HincII 1855 GTTAAC GT[CT]^[GA]AC
2035 GTTGAC
HindII 1855 GTTAAC GT[CT]^[GA]AC
2035 GTTGAC
KasI 859 GGCGCC G^GCGCC
2016 GGCGCC
MaeII 1142 ACGT A^CGT
1184 ACGT
MflI 1553 AGATCT [GA]^GATC[CT]
1750 AGATCC
MseI 512 TTAA T^TAA
1856 TTAA
NarI 859 GGCGCC GG^CGCC
2016 GGCGCC
NspI 221 GCATGT [GA]CATG^[CT]
1772 ACATGT
SacI 25 GAGCTC GAGCT^C
989 GAGCTC
SapI 27 GCTCTTC GCTCTTC(1/4)
171 GCTCTTC
SfoI 859 GGCGCC GGC^GCC
2016 GGCGCC
SmlI 872 CTCGAG C^T[CT][GA]AG
1414 CTTGAG
TaqI 873 TCGA T^CGA
1550 TCGA
TfiI 427 GAATC G^A[AT]TC
691 GAATC
Tru9I 512 TTAA T^TAA
1856 TTAA
Tsp509I 477 AATT ^AATT
1370 AATT
TthHB8I 873 TCGA T^CGA
1550 TCGA
XhoII 1553 AGATCT [GA]^GATC[CT]
1750 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcyI AluI AlwI AocI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcgI BcnI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TseI Tsp45I TspRI