Restriction map for
pFN21AE3409
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AocI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BglII BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspEI BspHI BspLU11I BspMI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
Cfr10I CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SspBI SspI
SwaI Tsp509I Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 9 CGCG CG^CG
AfaI 311 GTAC GT^AC
Alw44I 579 GTGCAC G^TGCAC
Aor51HI 53 AGCGCT AGC^GCT
ApaLI 579 GTGCAC G^TGCAC
AvaI 26 CTCGGG C^[CT]CG[GA]G
AvrII 660 CCTAGG C^CTAGG
BalI 894 TGGCCA TGG^CCA
BcnI 174 CCGGG CC^[GC]GG
BlnI 660 CCTAGG C^CTAGG
BlpI 268 GCTAAGC GC^T[ACGT]AGC
BpmI 868 CTGGAG CTGGAG(16/14)
Bpu1102I 268 GCTAAGC GC^T[ACGT]AGC
BsmAI 440 GAGAC GTCTC(1/5)
BsmFI 657 GTCCC GGGAC(10/14)
BsmI 780 GCATTC GAATGC(1/-1)
BsoBI 26 CTCGGG C^[CT]CG[GA]G
BspDI 634 ATCGAT AT^CGAT
BsrBI 640 GAGCGG CCGCTC(-3/-3)
BsrDI 791 CATTGC GCAATG(2/0)
BstUI 9 CGCG CG^CG
BstYI 392 GGATCT [GA]^GATC[CT]
CelII 268 GCTAAGC GC^T[ACGT]AGC
ClaI 634 ATCGAT AT^CGAT
DraII 696 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 821 CCGTGG C^C[GA][CT]GG
EaeI 894 TGGCCA [CT]^GGCC[GA]
Eco47III 53 AGCGCT AGC^GCT
EcoNI 357 CCTGACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 696 AGGCCCT [GA]G^G[ACGT]CC[CT]
HapII 174 CCGG C^CGG
HgaI 837 GCGTC GACGC(5/10)
HpaII 174 CCGG C^CGG
MflI 392 GGATCT [GA]^GATC[CT]
MluNI 894 TGGCCA TGG^CCA
MscI 894 TGGCCA TGG^CCA
MseI 490 TTAA T^TAA
MslI 522 CATCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 174 CCGG C^CGG
MvnI 9 CGCG CG^CG
NciI 174 CCGGG CC^[GC]GG
PleI 621 GAGTC GAGTC(4/5)
PshAI 645 GACTGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 865 CAGCTG CAG^CTG
RsaI 311 GTAC GT^AC
SacI 604 GAGCTC GAGCT^C
SapI 606 GCTCTTC GCTCTTC(1/4)
SbfI 555 CCTGCAGG CCTGCA^GG
SmlI 406 CTTGAG C^T[CT][GA]AG
SphI 517 GCATGC GCATG^C
Sse8387I 555 CCTGCAGG CCTGCA^GG
Tru9I 490 TTAA T^TAA
XhoII 392 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwI 366 GGATC GGATC(4/5)
392 GGATC
AspHI 579 GTGCAC G[AT]GC[AT]^C
604 GAGCTC
BfaI 106 CTAG C^TAG
661 CTAG
BsgI 616 GTGCAG GTGCAG(16/14)
745 GTGCAG
BsiHKAI 579 GTGCAC G[AT]GC[AT]^C
604 GAGCTC
BssSI 160 CACGAG CACGAG(-5/-1)
238 CTCGTG
Cac8I 517 GCATGC GC[ACGT]^[ACGT]GC
668 GCGTGC
DpnII 367 GATC ^GATC
393 GATC
EcoT14I 422 CCAAGG C^C[AT][AT]GG
660 CCTAGG
EcoT22I 202 ATGCAT ATGCA^T
519 ATGCAT
FauI 539 CCCGC CCCGC(4/6)
828 CCCGC
HaeII 53 AGCGCT [GA]GCGC^[CT]
873 GGCGCT
HphI 724 GGTGA GGTGA(8/7)
219 TCACC
MaeI 106 CTAG C^TAG
661 CTAG
MboI 367 GATC ^GATC
393 GATC
MspA1I 574 CAGCGG C[AC]G^C[GT]G
865 CAGCTG
MwoI 412 GCCATTCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
867 GCTGGAGGCGC
NdeII 367 GATC ^GATC
393 GATC
NsiI 202 ATGCAT ATGCA^T
519 ATGCAT
NspI 200 ACATGC [GA]CATG^[CT]
517 GCATGC
PflMI 69 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
886 CCAGCTCCTGG
Sau3AI 367 GATC ^GATC
393 GATC
StuI 278 AGGCCT AGG^CCT
402 AGGCCT
StyI 422 CCAAGG C^C[AT][AT]GG
660 CCTAGG
TaqI 635 TCGA T^CGA
811 TCGA
TfiI 149 GAATC G^A[AT]TC
510 GAATC
TspRI 625 CACTG CA[GC]TG[ACGT][ACGT]^
729 CAGTG
TthHB8I 635 TCGA T^CGA
811 TCGA
Van91I 69 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
886 CCAGCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanII
BbvI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI CfoI Cfr13I DdeI EarI
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HinfI ItaI Ksp632I MaeIII
MboII MnlI MvaI NlaIII NlaIV
PstI Sau96I ScrFI SfaNI SfcI
TseI Tsp45I