Restriction map for
pFN21AB8028
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# NO CUT ENZYMES ( 76 enzymes) #
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AatII AccIII AclI AflII AgeI
AscI AspI AviII AvrII BclI
BfrI BlnI BseAI BsgI BsiWI
BsmBI BspDI BspEI BspHI BsrBI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraI DraIII DrdI
EcoRV FbaI FseI FspI HincII
HindII HindIII HpaI KspI MluI
MroI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 71 enzymes) #
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Acc65I 98 GGTACC G^GTACC
AccII 64 CGCG CG^CG
AcyI 2617 GGCGCC G[GA]^CG[CT]C
AflIII 645 ACATGT A^C[GA][CT]GT
AhdI 2392 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1454 GTGCAC G^TGCAC
AocI 2452 CCTCAGG CC^T[ACGT]AGG
Aor51HI 460 AGCGCT AGC^GCT
ApaI 3232 GGGCCC GGGCC^C
ApaLI 1454 GTGCAC G^TGCAC
AseI 2107 ATTAAT AT^TAAT
AsnI 2107 ATTAAT AT^TAAT
Asp700I 2607 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 98 GGTACC G^GTACC
AspEI 2392 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2617 GGCGCC GGCGC^C
BbrPI 856 CACGTG CAC^GTG
BbvCI 3115 CCTCAGC CCTCAGC(-5/-2)
BcgI 2307 GCACATGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 328 GCCCAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2665 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2665 GCTGAGC GC^T[ACGT]AGC
BsaAI 856 CACGTG [CT]AC^GT[GA]
BsaBI 1578 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2617 GGCGCC G[GA]^CG[CT]C
BsaI 1779 GAGACC GGTCTC(1/5)
BsiEI 2302 CGGCCG CG[GA][CT]^CG
Bsp1407I 546 TGTACA T^GTACA
BspLU11I 645 ACATGT A^CATGT
BsrFI 2085 ACCGGC [GA]^CCGG[CT]
BsrGI 546 TGTACA T^GTACA
BstAPI 84 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1191 GGTTACC G^GT[ACGT]ACC
BstPI 1191 GGTTACC G^GT[ACGT]ACC
BstUI 64 CGCG CG^CG
Bsu36I 2452 CCTCAGG CC^T[ACGT]AGG
CelII 2665 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2085 ACCGGC [GA]^CCGG[CT]
EagI 2302 CGGCCG C^GGCCG
Eam1105I 2392 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2302 CGGCCG C^GGCCG
Eco47III 460 AGCGCT AGC^GCT
Eco52I 2302 CGGCCG C^GGCCG
Eco81I 2452 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1191 GGTTACC G^GT[ACGT]ACC
EcoRI 2460 GAATTC G^AATTC
EcoT22I 1025 ATGCAT ATGCA^T
HgaI 65 GCGTC GACGC(5/10)
Hin1I 2617 GGCGCC G[GA]^CG[CT]C
KasI 2617 GGCGCC G^GCGCC
KpnI 98 GGTACC GGTAC^C
MamI 1578 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 350 CAATTG C^AATTG
MunI 350 CAATTG C^AATTG
MvnI 64 CGCG CG^CG
NarI 2617 GGCGCC GG^CGCC
NsiI 1025 ATGCAT ATGCA^T
NspI 645 ACATGT [GA]CATG^[CT]
PflMI 349 CCAATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 856 CACGTG CAC^GTG
PmlI 856 CACGTG CAC^GTG
PshBI 2107 ATTAAT AT^TAAT
SfoI 2617 GGCGCC GGC^GCC
SmaI 110 CCCGGG CCC^GGG
SmlI 809 CTCAAG C^T[CT][GA]AG
SspBI 546 TGTACA T^GTACA
Van91I 349 CCAATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 509 CCACGCAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 110 CCCGGG C^CCGGG
XmaI 110 CCCGGG C^CCGGG
XmnI 2607 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AccI 787 GTAGAC GT^[AC][GT]AC
2977 GTAGAC
AcsI 1295 GAATTT [GA]^AATT[CT]
2460 GAATTC
ApoI 1295 GAATTT [GA]^AATT[CT]
2460 GAATTC
BamHI 177 GGATCC G^GATCC
1791 GGATCC
BglII 2074 AGATCT A^GATCT
3102 AGATCT
BpmI 1465 CTGGAG CTGGAG(16/14)
2713 CTGGAG
BsaWI 1324 ACCGGA [AT]^CCGG[AT]
3107 TCCGGT
BsmI 2901 GAATGC GAATGC(1/-1)
3200 GAATGC
BspMI 441 ACCTGC ACCTGC(4/8)
719 GCAGGT
BstXI 2019 CCACAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2231 CCAGCTCCTTGG
MaeII 857 ACGT A^CGT
3170 ACGT
NcoI 966 CCATGG C^CATGG
1724 CCATGG
PvuII 58 CAGCTG CAG^CTG
1645 CAGCTG
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BciVI BcnI
BfaI BmyI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII