Restriction map for
pFN21AE1643
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# NO CUT ENZYMES ( 182 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BcgI
BciVI BclI BcnI BfaI BfrI
BglII BlnI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspDI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DpnII DraI DraII DraIII
DrdI DsaI EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoT14I EcoT22I FbaI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HindIII HpaII HphI
KasI KpnI KspI MaeI MaeII
MboI MfeI MflI MluI MluNI
MroI MscI MslI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI Sau3AI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI TfiI
Tth111I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AciI 33 GCGG CCGC(-3/-1)
AfaI 285 GTAC GT^AC
AflIII 150 ACATGT A^C[GA][CT]GT
AhdI 109 GACCGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 109 GACCGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 28 CCTCAGC CCTCAGC(-5/-2)
BbvI 255 GCAGC GCAGC(8/12)
BglI 329 GCCAGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 119 CTCCAG CTGGAG(16/14)
BsaBI 49 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 157 GAGACC GGTCTC(1/5)
BsmI 41 GCATTC GAATGC(1/-1)
Bsp1407I 284 TGTACA T^GTACA
BspLU11I 150 ACATGT A^CATGT
BsrGI 284 TGTACA T^GTACA
BstF5I 414 CATCC GGATG(2/0)
BstXI 185 CCATCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 29 CTCAG C^T[ACGT]AG
Eam1105I 109 GACCGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 316 CTCTTC CTCTTC(1/4)
EcoRV 49 GATATC GAT^ATC
FauI 33 GCGGG CCCGC(4/6)
Fnu4HI 255 GCAGC GC^[ACGT]GC
FokI 414 CATCC GGATG(9/13)
HaeIII 196 GGCC GG^CC
HgaI 84 GACGC GACGC(5/10)
HincII 459 GTTAAC GT[CT]^[GA]AC
HindII 459 GTTAAC GT[CT]^[GA]AC
HpaI 459 GTTAAC GTT^AAC
ItaI 255 GCAGC GC^[ACGT]GC
Ksp632I 316 CTCTTC CTCTTC(1/4)
MaeIII 124 GTCAC ^GT[ACGT]AC
MamI 49 GATATCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI 150 ACATGT [GA]CATG^[CT]
PflMI 423 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 253 CTGCAG CTGCA^G
PvuII 256 CAGCTG CAG^CTG
RsaI 285 GTAC GT^AC
SfcI 253 CTGCAG C^T[GA][CT]AG
SmlI 163 CTTGAG C^T[CT][GA]AG
SspBI 284 TGTACA T^GTACA
TaqI 353 TCGA T^CGA
TseI 255 GCAGC G^C[AT]GC
Tsp45I 124 GTCAC ^GT[GC]AC
Tsp509I 18 AATT ^AATT
TspRI 251 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 353 TCGA T^CGA
Van91I 423 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 324 CCACAGCCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AluI 257 AGCT AG^CT
391 AGCT
AvaI 99 CCCGAG C^[CT]CG[GA]G
295 CCCGAG
BsmAI 157 GAGAC GTCTC(1/5)
117 GTCTC
BsmFI 239 GGGAC GGGAC(10/14)
97 GTCCC
BsoBI 99 CCCGAG C^[CT]CG[GA]G
295 CCCGAG
BsrI 429 ACTGG ACTGG(1/-1)
121 CCAGT
BssKI 192 CCTGG ^CC[ACGT]GG
370 CCTGG
BstNI 192 CCTGG CC^[AT]GG
370 CCTGG
EcoRII 192 CCTGG ^CC[AT]GG
370 CCTGG
HinfI 206 GACTC G^A[ACGT]TC
355 GAGTC
MboII 72 TCTTC GAAGA(8/7)
317 TCTTC
MseI 136 TTAA T^TAA
460 TTAA
MspA1I 31 CAGCGG C[AC]G^C[GT]G
256 CAGCTG
MvaI 192 CCTGG CC^[AT]GG
370 CCTGG
MwoI 246 GCCCACACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
329 GCCAGAGTGGC
NlaIII 143 CATG CATG^
151 CATG
NlaIV 179 GGAACC GG[ACGT]^[ACGT]CC
239 GGGACC
PleI 206 GACTC GAGTC(4/5)
355 GAGTC
ScrFI 192 CCTGG CC^[ACGT]GG
370 CCTGG
Tru9I 136 TTAA T^TAA
460 TTAA
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# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
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AvaII BsaJI BsiYI BslI Cfr13I
MnlI Sau96I