Restriction map for
pFN21AE1691
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BcgI
BclI BfrI BglI BglII BlnI
BpuAI BsaAI BsaBI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I ClaI CpoI DpnII
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MboI MfeI MflI
MluI MluNI MroI MscI MunI
NaeI NarI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tth111I XbaI
XcmI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 361 GACGTC GACGT^C
AccII 560 CGCG CG^CG
AcyI 361 GACGTC G[GA]^CG[CT]C
AfaI 477 GTAC GT^AC
AocI 255 CCTGAGG CC^T[ACGT]AGG
ApaI 442 GGGCCC GGGCC^C
BanI 221 GGTGCC G^G[CT][GA]CC
BbvCI 427 CCTCAGC CCTCAGC(-5/-2)
BciVI 419 GTATCC GTATCC(6/5)
BfaI 7 CTAG C^TAG
BlpI 298 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 298 GCTGAGC GC^T[ACGT]AGC
BsaHI 361 GACGTC G[GA]^CG[CT]C
BsaI 563 GAGACC GGTCTC(1/5)
BseRI 553 CTCCTC GAGGAG(10/8)
BsrFI 479 ACCGGC [GA]^CCGG[CT]
BstF5I 108 GGATG GGATG(2/0)
BstUI 560 CGCG CG^CG
BstXI 284 CCATGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 255 CCTGAGG CC^T[ACGT]AGG
CelII 298 GCTGAGC GC^T[ACGT]AGC
CfoI 279 GCGC GCG^C
Cfr10I 479 ACCGGC [GA]^CCGG[CT]
DrdI 589 GACCTGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 47 TGGCCG [CT]^GGCC[GA]
Eco81I 255 CCTGAGG CC^T[ACGT]AGG
EcoNI 167 CCTTTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 284 CCATGG C^C[AT][AT]GG
FauI 230 CCCGC CCCGC(4/6)
FokI 108 GGATG GGATG(9/13)
HaeII 278 AGCGCC [GA]GCGC^[CT]
HgaI 312 GACGC GACGC(5/10)
HhaI 279 GCGC GCG^C
Hin1I 361 GACGTC G[GA]^CG[CT]C
HinP1I 279 GCGC G^CGC
MaeI 7 CTAG C^TAG
MaeII 362 ACGT A^CGT
MseI 170 TTAA T^TAA
MslI 27 CACGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 560 CGCG CG^CG
NcoI 284 CCATGG C^CATGG
PaeR7I 520 CTCGAG C^TCGAG
PflMI 242 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 262 CAGCTG CAG^CTG
RsaI 477 GTAC GT^AC
SmaI 452 CCCGGG CCC^GGG
SmlI 520 CTCGAG C^T[CT][GA]AG
StyI 284 CCATGG C^C[AT][AT]GG
TaqI 521 TCGA T^CGA
Tru9I 170 TTAA T^TAA
Tsp509I 571 AATT ^AATT
TthHB8I 521 TCGA T^CGA
Van91I 242 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 520 CTCGAG C^TCGAG
XmaCI 452 CCCGGG C^CCGGG
XmaI 452 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaI 452 CCCGGG C^[CT]CG[GA]G
520 CTCGAG
BanII 98 GGGCTC G[GA]GC[CT]^C
442 GGGCCC
BmyI 98 GGGCTC G[AGT]GC[ACT]^C
442 GGGCCC
BsgI 24 CTGCAC GTGCAG(16/14)
388 CTGCAC
BsmAI 563 GAGAC GTCTC(1/5)
147 GTCTC
BsoBI 452 CCCGGG C^[CT]CG[GA]G
520 CTCGAG
Bsp1286I 98 GGGCTC G[AGT]GC[ACT]^C
442 GGGCCC
BspMI 218 GCAGGT ACCTGC(4/8)
267 GCAGGT
HinfI 196 GAGTC G^A[ACGT]TC
505 GAGTC
HphI 270 GGTGA GGTGA(8/7)
533 TCACC
MaeIII 64 GTGAC ^GT[ACGT]AC
532 GTCAC
MspA1I 262 CAGCTG C[AC]G^C[GT]G
335 CCGCTG
NlaIII 12 CATG CATG^
285 CATG
PleI 196 GAGTC GAGTC(4/5)
505 GAGTC
SfaNI 109 GATGC GCATC(5/9)
184 GATGC
StuI 164 AGGCCT AGG^CCT
524 AGGCCT
Tsp45I 64 GTGAC ^GT[GC]AC
532 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AlwNI AvaII BbvI
BcnI BpmI BsaJI BsiYI BslI
BsmFI BsrI BssKI BstNI Cac8I
Cfr13I DdeI DraII DsaI EarI
EcoO109I EcoRII Fnu4HI HaeIII HapII
HpaII ItaI Ksp632I MboII MnlI
MspI MvaI MwoI NciI NlaIV
PpuMI PstI Sau96I ScrFI SfcI
TseI TspRI