Restriction map for
pFN21AE1213
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HincII
HindII HindIII HpaI HphI KasI
KpnI Ksp632I KspI MaeI MaeIII
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tsp45I TspRI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 62 GAATTC [GA]^AATT[CT]
AcyI 310 GACGCC G[GA]^CG[CT]C
AfaI 318 GTAC GT^AC
AflIII 167 ACACGT A^C[GA][CT]GT
AluI 386 AGCT AG^CT
AlwI 401 GGATC GGATC(4/5)
ApoI 62 GAATTC [GA]^AATT[CT]
AspHI 265 GTGCTC G[AT]GC[AT]^C
AvaI 31 CCCGAG C^[CT]CG[GA]G
BanII 129 GGGCTC G[GA]GC[CT]^C
BbsI 181 GTCTTC GAAGAC(2/6)
BpuAI 181 GTCTTC GAAGAC(2/6)
BsaHI 310 GACGCC G[GA]^CG[CT]C
BsgI 382 GTGCAG GTGCAG(16/14)
BsiHKAI 265 GTGCTC G[AT]GC[AT]^C
BsoBI 31 CCCGAG C^[CT]CG[GA]G
BsrI 315 CCAGT ACTGG(1/-1)
BssHII 349 GCGCGC G^CGCGC
BstF5I 39 CATCC GGATG(2/0)
BstYI 401 GGATCT [GA]^GATC[CT]
DdeI 58 CTGAG C^T[ACGT]AG
DpnII 402 GATC ^GATC
DsaI 299 CCGTGG C^C[GA][CT]GG
EcoNI 360 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 62 GAATTC G^AATTC
FokI 39 CATCC GGATG(9/13)
Hin1I 310 GACGCC G[GA]^CG[CT]C
HinfI 391 GACTC G^A[ACGT]TC
MboI 402 GATC ^GATC
MflI 401 GGATCT [GA]^GATC[CT]
NdeII 402 GATC ^GATC
NlaIII 100 CATG CATG^
PleI 391 GACTC GAGTC(4/5)
PvuII 385 CAGCTG CAG^CTG
RsaI 318 GTAC GT^AC
Sau3AI 402 GATC ^GATC
TaqI 260 TCGA T^CGA
Tsp509I 63 AATT ^AATT
TthHB8I 260 TCGA T^CGA
XhoII 401 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BcnI 158 CCCGG CC^[GC]GG
229 CCGGG
BmyI 129 GGGCTC G[AGT]GC[ACT]^C
265 GTGCTC
BseRI 121 GAGGAG GAGGAG(10/8)
217 GAGGAG
Bsp1286I 129 GGGCTC G[AGT]GC[ACT]^C
265 GTGCTC
BspMI 107 ACCTGC ACCTGC(4/8)
92 GCAGGT
Cfr13I 210 GGGCC G^G[ACGT]CC
226 GGGCC
HapII 159 CCGG C^CGG
229 CCGG
HgaI 310 GACGC GACGC(5/10)
277 GCGTC
HpaII 159 CCGG C^CGG
229 CCGG
MboII 182 TCTTC GAAGA(8/7)
404 TCTTC
MspA1I 343 CCGCTG C[AC]G^C[GT]G
385 CAGCTG
MspI 159 CCGG C^CGG
229 CCGG
NciI 158 CCCGG CC^[GC]GG
229 CCGGG
Sau96I 210 GGGCC G^G[ACGT]CC
226 GGGCC
SfaNI 136 GATGC GCATC(5/9)
38 GCATC
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AccII AciI BbvI BsaJI BsiYI
BslI BsmFI BssKI BstNI BstUI
Cac8I CfoI EcoRII FauI Fnu4HI
HaeIII HhaI HinP1I ItaI MaeII
MnlI MvaI MvnI MwoI NlaIV
ScrFI TseI