Restriction map for
pFN21AE0982
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AhdI Alw44I ApaLI ApoI
AseI AsnI Asp700I AspEI AspI
AvrII BalI BamHI BcgI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BseAI BseRI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII Eam1105I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI HindIII
HpaI MfeI MluI MluNI MroI
MscI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SspBI SspI
SwaI TfiI Tsp509I Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AgeI 1109 ACCGGT A^CCGGT
AlwNI 795 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 952 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1217 AGCGCT AGC^GCT
ApaI 692 GGGCCC GGGCC^C
AscI 1402 GGCGCGCC GG^CGCGCC
AviII 821 TGCGCA TGC^GCA
BbrPI 1053 CACGTG CAC^GTG
BbsI 751 GAAGAC GAAGAC(2/6)
BbvCI 1168 GCTGAGG CCTCAGC(-5/-2)
BclI 525 TGATCA T^GATCA
BfaI 1225 CTAG C^TAG
BglI 1255 GCCTTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 751 GAAGAC GAAGAC(2/6)
BsaAI 1053 CACGTG [CT]AC^GT[GA]
BsaBI 195 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1109 ACCGGT [AT]^CCGG[AT]
BsmI 677 GAATGC GAATGC(1/-1)
BspLU11I 632 ACATGT A^CATGT
BstXI 1418 CCACGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 872 AGATCC [GA]^GATC[CT]
Bsu36I 952 CCTGAGG CC^T[ACGT]AGG
DrdI 223 GACAACATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1217 AGCGCT AGC^GCT
Eco81I 952 CCTGAGG CC^T[ACGT]AGG
FbaI 525 TGATCA T^GATCA
FspI 821 TGCGCA TGC^GCA
HgaI 1120 GACGC GACGC(5/10)
HincII 61 GTTGAC GT[CT]^[GA]AC
HindII 61 GTTGAC GT[CT]^[GA]AC
HinfI 1198 GACTC G^A[ACGT]TC
KspI 402 CCGCGG CCGC^GG
MaeI 1225 CTAG C^TAG
MamI 195 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 872 AGATCC [GA]^GATC[CT]
MseI 884 TTAA T^TAA
PinAI 1109 ACCGGT A^CCGGT
PleI 1198 GACTC GAGTC(4/5)
PmaCI 1053 CACGTG CAC^GTG
PmlI 1053 CACGTG CAC^GTG
PstI 271 CTGCAG CTGCA^G
SacII 402 CCGCGG CCGC^GG
SapI 367 GAAGAGC GCTCTTC(1/4)
SbfI 270 CCTGCAGG CCTGCA^GG
SfcI 271 CTGCAG C^T[GA][CT]AG
SphI 372 GCATGC GCATG^C
Sse8387I 270 CCTGCAGG CCTGCA^GG
Tru9I 884 TTAA T^TAA
Tsp45I 14 GTGAC ^GT[GC]AC
XhoII 872 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
Acc65I 578 GGTACC G^GTACC
1067 GGTACC
Asp718I 578 GGTACC G^GTACC
1067 GGTACC
BsaI 325 GAGACC GGTCTC(1/5)
727 GAGACC
BsmAI 325 GAGAC GTCTC(1/5)
727 GAGAC
KpnI 578 GGTACC GGTAC^C
1067 GGTACC
MaeIII 14 GTGAC ^GT[ACGT]AC
568 GTAAC
MslI 627 CATCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
941 CATACTCGTG
NcoI 890 CCATGG C^CATGG
1244 CCATGG
NspI 372 GCATGC [GA]CATG^[CT]
632 ACATGT
PflMI 69 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
696 CCATGAGCTGG
SmlI 409 CTCAAG C^T[CT][GA]AG
490 CTCAAG
StuI 814 AGGCCT AGG^CCT
1739 AGGCCT
Van91I 69 CCAACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
696 CCATGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BmyI BpmI BsaHI BsaJI BsgI
BsiEI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssHII BssKI BssSI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII HphI ItaI KasI
Ksp632I MaeII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfoI
StyI TaqI TseI TspRI TthHB8I
XcmI