Restriction map for
pFN21AE3217
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BciVI BclI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CfoI ClaI CpoI DraI
DraII DraIII EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO109I EcoO65I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HpaI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MroI MseI
MspA1I MunI MvnI NarI NcoI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tru9I Tth111I TthHB8I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Alw44I 241 GTGCAC G^TGCAC
AlwI 258 GATCC GGATC(4/5)
AocI 301 CCTGAGG CC^T[ACGT]AGG
ApaLI 241 GTGCAC G^TGCAC
AvaI 185 CTCGGG C^[CT]CG[GA]G
AvaII 93 GGACC G^G[AT]CC
BbvCI 614 CCTCAGC CCTCAGC(-5/-2)
BcgI 208 CGAGAGCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 978 GAGACC GGTCTC(1/5)
BsgI 490 GTGCAG GTGCAG(16/14)
BsmFI 531 GTCCC GGGAC(10/14)
BsoBI 185 CTCGGG C^[CT]CG[GA]G
BspHI 825 TCATGA T^CATGA
BsrFI 200 GCCGGC [GA]^CCGG[CT]
BstYI 257 AGATCC [GA]^GATC[CT]
Bsu36I 301 CCTGAGG CC^T[ACGT]AGG
Cfr10I 200 GCCGGC [GA]^CCGG[CT]
DpnII 258 GATC ^GATC
DsaI 800 CCACGG C^C[GA][CT]GG
Eco81I 301 CCTGAGG CC^T[ACGT]AGG
EcoNI 297 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 627 GATATC GAT^ATC
EcoT22I 986 ATGCAT ATGCA^T
HincII 880 GTTGAC GT[CT]^[GA]AC
HindII 880 GTTGAC GT[CT]^[GA]AC
MboI 258 GATC ^GATC
MflI 257 AGATCC [GA]^GATC[CT]
NaeI 200 GCCGGC GCC^GGC
NdeII 258 GATC ^GATC
NgoMIV 200 GCCGGC G^CCGGC
NsiI 986 ATGCAT ATGCA^T
PflMI 337 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 725 CTGCAG CTGCA^G
RcaI 825 TCATGA T^CATGA
Sau3AI 258 GATC ^GATC
SfcI 725 CTGCAG C^T[GA][CT]AG
Tsp45I 358 GTGAC ^GT[GC]AC
Van91I 337 CCAGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 543 CCAGGAGAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 257 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 256 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
283 CAGTTCCTG
Asp700I 49 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1173 GAAGGCCTTC
BalI 290 TGGCCA TGG^CCA
420 TGGCCA
BcnI 486 CCCGG CC^[GC]GG
634 CCGGG
BfaI 58 CTAG C^TAG
887 CTAG
BlpI 1190 GCTCAGC GC^T[ACGT]AGC
1358 GCTCAGC
Bpu1102I 1190 GCTCAGC GC^T[ACGT]AGC
1358 GCTCAGC
BstXI 14 CCACAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
96 CCAGGACTTTGG
CelII 1190 GCTCAGC GC^T[ACGT]AGC
1358 GCTCAGC
Cfr13I 93 GGACC G^G[ACGT]CC
181 GGCCC
DrdI 522 GACTCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
764 GACATTTCAGTC
EcoT14I 248 CCAAGG C^C[AT][AT]GG
397 CCAAGG
HindIII 314 AAGCTT A^AGCTT
680 AAGCTT
HphI 1057 GGTGA GGTGA(8/7)
1367 TCACC
MaeI 58 CTAG C^TAG
887 CTAG
MaeIII 164 GTTAC ^GT[ACGT]AC
358 GTGAC
MluNI 290 TGGCCA TGG^CCA
420 TGGCCA
MscI 290 TGGCCA TGG^CCA
420 TGGCCA
MslI 826 CATGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1051 CATAGTGGTG
NciI 486 CCCGG CC^[GC]GG
634 CCGGG
NlaIV 125 GGAGCC GG[ACGT]^[ACGT]CC
393 GGAGCC
NspI 988 GCATGT [GA]CATG^[CT]
1249 GCATGT
Sau96I 93 GGACC G^G[ACGT]CC
181 GGCCC
StyI 248 CCAAGG C^C[AT][AT]GG
397 CCAAGG
XmnI 49 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1173 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AluI ApoI AspHI BanII
BbvI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
DdeI EaeI EcoRI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MboII MnlI MspI MvaI
MwoI NlaIII PleI SacI ScrFI
SfaNI StuI TfiI TseI Tsp509I
TspRI