##################################
# NO CUT ENZYMES (  187 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BseAI       
BseRI       BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        ClaI        CpoI        
DpnII       DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        KasI        Ksp632I     KspI        MaeII       
MamI        MboI        MboII       MfeI        MflI        
MluI        MroI        MseI        MslI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StyI        SwaI        TaqI        TfiI        
Tru9I       Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I        379  GGTACC               G^GTACC
AfaI          380  GTAC                 GT^AC
AgeI          184  ACCGGT               A^CCGGT
Asp718I       379  GGTACC               G^GTACC
AspHI         336  GAGCAC               G[AT]GC[AT]^C
AvaII          53  GGACC                G^G[AT]CC
BalI          309  TGGCCA               TGG^CCA
BanII         520  GAGCCC               G[GA]GC[CT]^C
BglI          311  GCCAGGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI           63  CTCCAG               CTGGAG(16/14)
BsaJI         446  CCGAGG               C^C[ACGT][ACGT]GG
BsaWI         184  ACCGGT               [AT]^CCGG[AT]
BsiHKAI       336  GAGCAC               G[AT]GC[AT]^C
BsrFI         184  ACCGGT               [GA]^CCGG[CT]
BssKI         312  CCAGG                ^CC[ACGT]GG
BstNI         312  CCAGG                CC^[AT]GG
Cfr10I        184  ACCGGT               [GA]^CCGG[CT]
Cfr13I         53  GGACC                G^G[ACGT]CC
DdeI          518  CTGAG                C^T[ACGT]AG
DraII          52  GGGACCT              [GA]G^G[ACGT]CC[CT]
EaeI          309  TGGCCA               [CT]^GGCC[GA]
EcoO109I       52  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRII        312  CCAGG                ^CC[AT]GG
HapII         185  CCGG                 C^CGG
HinfI         199  GACTC                G^A[ACGT]TC
HpaII         185  CCGG                 C^CGG
HphI          405  GGTGA                GGTGA(8/7)
KpnI          379  GGTACC               GGTAC^C
MaeIII        197  GTGAC                ^GT[ACGT]AC
MluNI         309  TGGCCA               TGG^CCA
MscI          309  TGGCCA               TGG^CCA
MspI          185  CCGG                 C^CGG
MvaI          312  CCAGG                CC^[AT]GG
PinAI         184  ACCGGT               A^CCGGT
PleI          199  GACTC                GAGTC(4/5)
PpuMI          52  GGGACCT              [GA]G^G[AT]CC[CT]
RsaI          380  GTAC                 GT^AC
Sau96I         53  GGACC                G^G[ACGT]CC
ScrFI         312  CCAGG                CC^[ACGT]GG
StuI          449  AGGCCT               AGG^CCT
Tsp45I        197  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

BanI          353  GGTGCC               G^G[CT][GA]CC
              379  GGTACC              
BmyI          336  GAGCAC               G[AGT]GC[ACT]^C
              520  GAGCCC              
BsaI           91  GAGACC               GGTCTC(1/5)
              121  GAGACC              
BsgI          411  CTGCAC               GTGCAG(16/14)
              436  CTGCAC              
BsmAI          91  GAGAC                GTCTC(1/5)
              121  GAGAC               
BsmFI          52  GGGAC                GGGAC(10/14)
              190  GGGAC               
Bsp1286I      336  GAGCAC               G[AGT]GC[ACT]^C
              520  GAGCCC              
BspMI          55  ACCTGC               ACCTGC(4/8)
              382  ACCTGC              
BstF5I        422  CATCC                GGATG(2/0)
              467  CATCC               
FokI          422  CATCC                GGATG(9/13)
              467  CATCC               
TspRI          48  CAGTG                CA[GC]TG[ACGT][ACGT]^
              324  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   19 enzymes) #
#########################################

AciI        AluI        AlwNI       BbvI        BfaI        
BsiYI       BslI        BsrI        Cac8I       Fnu4HI      
HaeIII      ItaI        MaeI        MnlI        MspA1I      
MwoI        NlaIV       PvuII       TseI