Restriction map for
pFN21AE6073
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbsI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HapII HhaI HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MflI MluI
MroI MseI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SwaI TaqI Tru9I Tth111I TthHB8I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AcyI 310 GACGCC G[GA]^CG[CT]C
AfaI 204 GTAC GT^AC
AflIII 745 ACGTGT A^C[GA][CT]GT
AlwNI 26 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
BanII 181 GAGCCC G[GA]GC[CT]^C
BbvCI 153 CCTCAGC CCTCAGC(-5/-2)
BfaI 76 CTAG C^TAG
BpmI 594 CTCCAG CTGGAG(16/14)
BsaHI 310 GACGCC G[GA]^CG[CT]C
BsmBI 277 CGTCTC CGTCTC(1/5)
BsrDI 265 GCAATG GCAATG(2/0)
DpnII 953 GATC ^GATC
DraIII 502 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
FauI 428 CCCGC CCCGC(4/6)
Hin1I 310 GACGCC G[GA]^CG[CT]C
MaeI 76 CTAG C^TAG
MboI 953 GATC ^GATC
MslI 426 CACCCGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 377 CCATGG C^CATGG
NdeII 953 GATC ^GATC
PstI 695 CTGCAG CTGCA^G
PvuII 692 CAGCTG CAG^CTG
RsaI 204 GTAC GT^AC
Sau3AI 953 GATC ^GATC
SspI 936 AATATT AAT^ATT
StuI 734 AGGCCT AGG^CCT
TfiI 707 GAATC G^A[AT]TC
Tsp509I 300 AATT ^AATT
XcmI 339 CCACCTTCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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Asp700I 707 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
732 GAAGGCCTTC
AspHI 112 GTGCTC G[AT]GC[AT]^C
923 GTGCTC
AvaII 35 GGACC G^G[AT]CC
44 GGACC
BalI 398 TGGCCA TGG^CCA
509 TGGCCA
BbrPI 685 CACGTG CAC^GTG
744 CACGTG
BsaAI 685 CACGTG [CT]AC^GT[GA]
744 CACGTG
BsiHKAI 112 GTGCTC G[AT]GC[AT]^C
923 GTGCTC
BsmAI 807 GAGAC GTCTC(1/5)
278 GTCTC
BsmFI 34 GGGAC GGGAC(10/14)
285 GTCCC
DsaI 377 CCATGG C^C[GA][CT]GG
764 CCGTGG
EaeI 398 TGGCCA [CT]^GGCC[GA]
509 TGGCCA
EcoT14I 377 CCATGG C^C[AT][AT]GG
470 CCTTGG
HgaI 310 GACGC GACGC(5/10)
466 GCGTC
MaeII 686 ACGT A^CGT
745 ACGT
MluNI 398 TGGCCA TGG^CCA
509 TGGCCA
MscI 398 TGGCCA TGG^CCA
509 TGGCCA
MspA1I 47 CCGCTG C[AC]G^C[GT]G
692 CAGCTG
PleI 392 GACTC GAGTC(4/5)
435 GAGTC
PmaCI 685 CACGTG CAC^GTG
744 CACGTG
PmlI 685 CACGTG CAC^GTG
744 CACGTG
SapI 327 GCTCTTC GCTCTTC(1/4)
576 GCTCTTC
SfaNI 93 GATGC GCATC(5/9)
158 GCATC
StyI 377 CCATGG C^C[AT][AT]GG
470 CCTTGG
XmnI 707 GAATCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
732 GAAGGCCTTC
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI BbvI BmyI BsaJI
BseRI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI EarI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeIII MboII MnlI MvaI MwoI
NlaIII NlaIV PflMI Sau96I ScrFI
SfcI TseI Tsp45I TspRI Van91I