Restriction map for
pFN21AE5175
##################################
# NO CUT ENZYMES ( 201 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsgI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssKI
BssSI Bst1107I BstBI BstEII BstNI
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT22I FbaI FseI FspI HaeII
HaeIII HhaI HinP1I HincII HindII
HindIII HinfI HpaI KasI KpnI
KspI MaeI MaeIII MamI MfeI
MflI MluI MluNI MroI MscI
MspA1I MunI MvaI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI Sau96I SbfI
ScaI ScrFI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tsp45I Tsp509I Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 63 GACGTC GACGT^C
AcyI 63 GACGTC G[GA]^CG[CT]C
AfaI 131 GTAC GT^AC
AspHI 213 GAGCTC G[AT]GC[AT]^C
BbsI 312 GAAGAC GAAGAC(2/6)
BpuAI 312 GAAGAC GAAGAC(2/6)
BsaHI 63 GACGTC G[GA]^CG[CT]C
BsaJI 218 CCAAGG C^C[ACGT][ACGT]GG
BsiEI 102 CGGTCG CG[GA][CT]^CG
BsiHKAI 213 GAGCTC G[AT]GC[AT]^C
BsmAI 290 GAGAC GTCTC(1/5)
BspMI 348 ACCTGC ACCTGC(4/8)
BstAPI 343 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 273 GGATG GGATG(2/0)
DpnII 198 GATC ^GATC
EarI 285 GAAGAG CTCTTC(1/4)
EcoT14I 218 CCAAGG C^C[AT][AT]GG
FauI 151 CCCGC CCCGC(4/6)
FokI 273 GGATG GGATG(9/13)
HapII 60 CCGG C^CGG
HgaI 74 GCGTC GACGC(5/10)
Hin1I 63 GACGTC G[GA]^CG[CT]C
HpaII 60 CCGG C^CGG
Ksp632I 285 GAAGAG CTCTTC(1/4)
MaeII 64 ACGT A^CGT
MboI 198 GATC ^GATC
MseI 172 TTAA T^TAA
MslI 204 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 60 CCGG C^CGG
NdeII 198 GATC ^GATC
NlaIII 270 CATG CATG^
RsaI 131 GTAC GT^AC
SacI 213 GAGCTC GAGCT^C
Sau3AI 198 GATC ^GATC
StyI 218 CCAAGG C^C[AT][AT]GG
TaqI 105 TCGA T^CGA
Tru9I 172 TTAA T^TAA
TspRI 204 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 105 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AciI 152 CCGC CCGC(-3/-1)
353 CCGC
AlwNI 22 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
344 CAGCACCTG
BanII 148 GAGCCC G[GA]GC[CT]^C
213 GAGCTC
BmyI 148 GAGCCC G[AGT]GC[ACT]^C
213 GAGCTC
BsiYI 60 CCGGACGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
121 CCAAAGAAGGG
BslI 60 CCGGACGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
121 CCAAAGAAGGG
Bsp1286I 148 GAGCCC G[AGT]GC[ACT]^C
213 GAGCTC
Cac8I 17 GCGAGC GC[ACGT]^[ACGT]GC
150 GCCCGC
DdeI 259 CTTAG C^T[ACGT]AG
308 CTAAG
HphI 210 GGTGA GGTGA(8/7)
83 TCACC
MnlI 255 CCTC CCTC(7/6)
139 GAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
AluI BbvI Fnu4HI ItaI MboII
MwoI TseI