Restriction map for
pFN21AE1226
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# NO CUT ENZYMES ( 143 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AgeI AhdI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MroI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI ScaI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 274 GGTACC G^GTACC
AccI 667 GTAGAC GT^[AC][GT]AC
AccII 200 CGCG CG^CG
AflIII 199 ACGCGT A^C[GA][CT]GT
Alw44I 256 GTGCAC G^TGCAC
AlwNI 884 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 256 GTGCAC G^TGCAC
Asp718I 274 GGTACC G^GTACC
BbrPI 679 CACGTG CAC^GTG
BfaI 562 CTAG C^TAG
BseRI 769 GAGGAG GAGGAG(10/8)
BsmBI 892 GAGACG CGTCTC(1/5)
BspMI 59 ACCTGC ACCTGC(4/8)
BsrBI 462 CCGCTC CCGCTC(-3/-3)
BsrDI 183 CATTGC GCAATG(2/0)
BstUI 200 CGCG CG^CG
BstXI 149 CCACCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 620 GCGC GCG^C
DraIII 710 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 478 CTCTTC CTCTTC(1/4)
EcoT14I 123 CCATGG C^C[AT][AT]GG
HhaI 620 GCGC GCG^C
HinP1I 620 GCGC G^CGC
KpnI 274 GGTACC GGTAC^C
Ksp632I 478 CTCTTC CTCTTC(1/4)
MaeI 562 CTAG C^TAG
MluI 199 ACGCGT A^CGCGT
MvnI 200 CGCG CG^CG
NcoI 123 CCATGG C^CATGG
NspI 702 GCATGT [GA]CATG^[CT]
PmaCI 679 CACGTG CAC^GTG
PmlI 679 CACGTG CAC^GTG
SapI 477 GCTCTTC GCTCTTC(1/4)
SbfI 507 CCTGCAGG CCTGCA^GG
SexAI 307 ACCTGGT A^CC[AT]GGT
SmaI 89 CCCGGG CCC^GGG
Sse8387I 507 CCTGCAGG CCTGCA^GG
StyI 123 CCATGG C^C[AT][AT]GG
XmaCI 89 CCCGGG C^CCGGG
XmaI 89 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AfaI 275 GTAC GT^AC
732 GTAC
AvaI 65 CTCGGG C^[CT]CG[GA]G
89 CCCGGG
AvaII 46 GGACC G^G[AT]CC
459 GGACC
BalI 374 TGGCCA TGG^CCA
437 TGGCCA
BpmI 870 CTCCAG CTGGAG(16/14)
754 CTGGAG
BsaAI 679 CACGTG [CT]AC^GT[GA]
733 TACGTG
BsoBI 65 CTCGGG C^[CT]CG[GA]G
89 CCCGGG
EaeI 374 TGGCCA [CT]^GGCC[GA]
437 TGGCCA
HinfI 179 GAGTC G^A[ACGT]TC
868 GACTC
MluNI 374 TGGCCA TGG^CCA
437 TGGCCA
MscI 374 TGGCCA TGG^CCA
437 TGGCCA
MslI 249 CATCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
640 CACCGTGATG
PleI 868 GACTC GAGTC(4/5)
179 GAGTC
PvuII 191 CAGCTG CAG^CTG
361 CAGCTG
RsaI 275 GTAC GT^AC
732 GTAC
SacI 475 GAGCTC GAGCT^C
832 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AlwI AspHI BanI
BanII BbvI BcnI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DsaI EcoRII FauI Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI Tsp45I TspRI XhoII