Restriction map for
pFN21AE6638
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AgeI Alw44I AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspI AviII AvrII BalI
BamHI BbeI BbsI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsmAI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrFI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI Ksp632I KspI MaeI MaeIII
MamI MfeI MflI MluI MluNI
MroI MscI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspI StuI
StyI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 242 GGTACC G^GTACC
AccI 274 GTCTAC GT^[AC][GT]AC
AhdI 121 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 412 AGCGCT AGC^GCT
Asp718I 242 GGTACC G^GTACC
AspEI 121 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 660 GTGCTC G[AT]GC[AT]^C
AvaI 663 CTCGGG C^[CT]CG[GA]G
AvaII 336 GGACC G^G[AT]CC
BanI 242 GGTACC G^G[CT][GA]CC
BbrPI 133 CACGTG CAC^GTG
BclI 680 TGATCA T^GATCA
BsiEI 357 CGACCG CG[GA][CT]^CG
BsiHKAI 660 GTGCTC G[AT]GC[AT]^C
BsiWI 293 CGTACG C^GTACG
BsoBI 663 CTCGGG C^[CT]CG[GA]G
Bsp1407I 137 TGTACA T^GTACA
BsrBI 344 GAGCGG CCGCTC(-3/-3)
BsrGI 137 TGTACA T^GTACA
BssHII 96 GCGCGC G^CGCGC
BstXI 470 CCAAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 194 CTGAG C^T[ACGT]AG
DraII 435 GGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 535 CGGCCA [CT]^GGCC[GA]
Eam1105I 121 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 412 AGCGCT AGC^GCT
EcoO109I 435 GGGGCCT [GA]G^G[ACGT]CC[CT]
FauI 20 CCCGC CCCGC(4/6)
FbaI 680 TGATCA T^GATCA
HaeII 412 AGCGCT [GA]GCGC^[CT]
HgaI 210 GCGTC GACGC(5/10)
HinfI 466 GACTC G^A[ACGT]TC
KpnI 242 GGTACC GGTAC^C
MboII 513 GAAGA GAAGA(8/7)
MslI 153 CATCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 500 GCATGC [GA]CATG^[CT]
PleI 466 GACTC GAGTC(4/5)
PmaCI 133 CACGTG CAC^GTG
PmlI 133 CACGTG CAC^GTG
PstI 427 CTGCAG CTGCA^G
SfaNI 625 GATGC GCATC(5/9)
SfcI 427 CTGCAG C^T[GA][CT]AG
SphI 500 GCATGC GCATG^C
SspBI 137 TGTACA T^GTACA
TspRI 321 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 470 CCAAGTGTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 10 enzymes) #
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AlwI 366 GATCC GGATC(4/5)
692 GGATC
BanII 444 GGGCTC G[GA]GC[CT]^C
637 GGGCTC
BpmI 247 CTGGAG CTGGAG(16/14)
604 CTGGAG
BsaAI 133 CACGTG [CT]AC^GT[GA]
291 CACGTA
BsrI 689 ACTGG ACTGG(1/-1)
320 CCAGT
DsaI 381 CCACGG C^C[GA][CT]GG
590 CCACGG
HphI 303 GGTGA GGTGA(8/7)
560 TCACC
PvuII 397 CAGCTG CAG^CTG
430 CAGCTG
TaqI 35 TCGA T^CGA
163 TCGA
TthHB8I 35 TCGA T^CGA
163 TCGA
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwNI BbvI BcnI BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DpnII EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI MaeII MboI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI TseI