Restriction map for
pFN21AE3695
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AgeI Aor51HI ApoI AscI
AseI AsnI AspI AviII AvrII
BamHI BbeI BbvCI BciVI BclI
BfrI BlnI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DraIII Eco47III EcoRI EcoT22I FbaI
FseI FspI HaeII HindIII HpaI
KasI KspI MfeI MluI MroI
MseI MunI NaeI NarI NgoMIV
NheI NotI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SspBI
SwaI Tru9I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 1028 GGTACC G^GTACC
AhdI 961 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1651 GTGCAC G^TGCAC
ApaI 49 GGGCCC GGGCC^C
ApaLI 1651 GTGCAC G^TGCAC
Asp700I 357 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1028 GGTACC G^GTACC
AspEI 961 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 134 TGGCCA TGG^CCA
BbrPI 289 CACGTG CAC^GTG
BbsI 66 GAAGAC GAAGAC(2/6)
BcgI 1911 CGACCCGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1598 AGATCT A^GATCT
BpuAI 66 GAAGAC GAAGAC(2/6)
BsaAI 289 CACGTG [CT]AC^GT[GA]
BsaBI 198 GATCGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1016 ACCGGA [AT]^CCGG[AT]
BsiEI 579 CGGCCG CG[GA][CT]^CG
BssHII 26 GCGCGC G^CGCGC
BstAPI 957 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1287 GGTGACC G^GT[ACGT]ACC
BstPI 1287 GGTGACC G^GT[ACGT]ACC
BstXI 1199 CCATTGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 1524 GACACCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 579 CGGCCG C^GGCCG
Eam1105I 961 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 307 CTCTTC CTCTTC(1/4)
EclXI 579 CGGCCG C^GGCCG
Eco52I 579 CGGCCG C^GGCCG
EcoO65I 1287 GGTGACC G^GT[ACGT]ACC
EcoRV 1774 GATATC GAT^ATC
HincII 148 GTCAAC GT[CT]^[GA]AC
HindII 148 GTCAAC GT[CT]^[GA]AC
KpnI 1028 GGTACC GGTAC^C
Ksp632I 307 CTCTTC CTCTTC(1/4)
MamI 198 GATCGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 134 TGGCCA TGG^CCA
MscI 134 TGGCCA TGG^CCA
NcoI 1145 CCATGG C^CATGG
NruI 200 TCGCGA TCG^CGA
PflMI 126 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 289 CACGTG CAC^GTG
PmlI 289 CACGTG CAC^GTG
PshAI 1338 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 306 GCTCTTC GCTCTTC(1/4)
SbfI 492 CCTGCAGG CCTGCA^GG
SmaI 1283 CCCGGG CCC^GGG
SphI 914 GCATGC GCATG^C
Sse8387I 492 CCTGCAGG CCTGCA^GG
SspI 266 AATATT AAT^ATT
StuI 1254 AGGCCT AGG^CCT
TfiI 1668 GATTC G^A[AT]TC
Tsp509I 1989 AATT ^AATT
Van91I 126 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1943 CCACTAACTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1283 CCCGGG C^CCGGG
XmaI 1283 CCCGGG C^CCGGG
XmnI 357 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AocI 343 CCTAAGG CC^T[ACGT]AGG
1250 CCTCAGG
BfaI 283 CTAG C^TAG
781 CTAG
BsgI 241 CTGCAC GTGCAG(16/14)
859 GTGCAG
BsrBI 867 CCGCTC CCGCTC(-3/-3)
190 GAGCGG
BsrI 924 ACTGG ACTGG(1/-1)
1673 CCAGT
Bsu36I 343 CCTAAGG CC^T[ACGT]AGG
1250 CCTCAGG
DsaI 1145 CCATGG C^C[GA][CT]GG
1727 CCACGG
Eco81I 343 CCTAAGG CC^T[ACGT]AGG
1250 CCTCAGG
EcoNI 1301 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1581 CCTGGAGAAGG
MaeI 283 CTAG C^TAG
781 CTAG
MslI 1146 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1200 CATTGAGATG
NdeI 408 CATATG CA^TATG
801 CATATG
NspI 701 ACATGC [GA]CATG^[CT]
914 GCATGC
PstI 493 CTGCAG CTGCA^G
955 CTGCAG
SacI 304 GAGCTC GAGCT^C
1629 GAGCTC
SexAI 275 ACCTGGT A^CC[AT]GGT
317 ACCTGGT
SfcI 493 CTGCAG C^T[GA][CT]AG
955 CTGCAG
TspRI 112 CACTG CA[GC]TG[ACGT][ACGT]^
923 CACTG
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TseI Tsp45I
TthHB8I XhoII