Restriction map for
pFN21AE3157
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI AocI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BclI BfrI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BssHII
Bst1107I BstBI BstXI BstYI BstZ17I
Bsu36I ClaI DraI DraIII Eam1105I
Eco81I EcoNI EcoRI EcoRV FbaI
FseI FspI HincII HindII HpaI
KpnI KspI MamI MfeI MflI
MluI MluNI MroI MscI MseI
MunI NdeI NheI NotI NruI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I Tsp509I TthHB8I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Alw44I 397 GTGCAC G^TGCAC
Aor51HI 752 AGCGCT AGC^GCT
ApaI 311 GGGCCC GGGCC^C
ApaLI 397 GTGCAC G^TGCAC
AspI 187 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 218 GTATCC GTATCC(6/5)
BfaI 766 CTAG C^TAG
BglI 662 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1085 GAGACC GGTCTC(1/5)
BsiEI 745 CGGCCG CG[GA][CT]^CG
BsmI 281 GCATTC GAATGC(1/-1)
BsrBI 82 GAGCGG CCGCTC(-3/-3)
BstAPI 399 GCACGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 879 GGTGACC G^GT[ACGT]ACC
BstPI 879 GGTGACC G^GT[ACGT]ACC
CpoI 185 CGGACCG CG^G[AT]CCG
DraII 310 GGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 157 GACGTTTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1115 CCATGG C^C[GA][CT]GG
EagI 745 CGGCCG C^GGCCG
EclXI 745 CGGCCG C^GGCCG
Eco47III 752 AGCGCT AGC^GCT
Eco52I 745 CGGCCG C^GGCCG
EcoO109I 310 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 879 GGTGACC G^GT[ACGT]ACC
EcoT14I 1115 CCATGG C^C[AT][AT]GG
EcoT22I 853 ATGCAT ATGCA^T
HgaI 191 GCGTC GACGC(5/10)
HindIII 22 AAGCTT A^AGCTT
MaeI 766 CTAG C^TAG
MaeIII 880 GTGAC ^GT[ACGT]AC
MslI 640 CACTATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1115 CCATGG C^CATGG
NsiI 853 ATGCAT ATGCA^T
PflFI 187 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 828 CTGCAG CTGCA^G
RsrII 185 CGGACCG CG^G[AT]CCG
SapI 570 GCTCTTC GCTCTTC(1/4)
SmlI 25 CTTGAG C^T[CT][GA]AG
StyI 1115 CCATGG C^C[AT][AT]GG
Tsp45I 880 GTGAC ^GT[GC]AC
Tth111I 187 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 31 enzymes) #
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AcyI 608 GGCGCC G[GA]^CG[CT]C
846 GGCGCC
AlwNI 595 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
793 CAGGAGCTG
AvaI 359 CCCGGG C^[CT]CG[GA]G
1037 CCCGGG
BbeI 608 GGCGCC GGCGC^C
846 GGCGCC
BbsI 1098 GAAGAC GAAGAC(2/6)
545 GTCTTC
BlpI 498 GCTGAGC GC^T[ACGT]AGC
760 GCTCAGC
Bpu1102I 498 GCTGAGC GC^T[ACGT]AGC
760 GCTCAGC
BpuAI 1098 GAAGAC GAAGAC(2/6)
545 GTCTTC
BsaHI 608 GGCGCC G[GA]^CG[CT]C
846 GGCGCC
BsgI 484 CTGCAC GTGCAG(16/14)
712 GTGCAG
BsoBI 359 CCCGGG C^[CT]CG[GA]G
1037 CCCGGG
BssSI 169 CACGAG CACGAG(-5/-1)
487 CACGAG
BstF5I 447 GGATG GGATG(2/0)
467 GGATG
CelII 498 GCTGAGC GC^T[ACGT]AGC
760 GCTCAGC
EaeI 745 CGGCCG [CT]^GGCC[GA]
941 CGGCCA
EarI 571 CTCTTC CTCTTC(1/4)
64 GAAGAG
FokI 447 GGATG GGATG(9/13)
467 GGATG
Hin1I 608 GGCGCC G[GA]^CG[CT]C
846 GGCGCC
KasI 608 GGCGCC G^GCGCC
846 GGCGCC
Ksp632I 571 CTCTTC CTCTTC(1/4)
64 GAAGAG
NaeI 555 GCCGGC GCC^GGC
781 GCCGGC
NarI 608 GGCGCC GG^CGCC
846 GGCGCC
NgoMIV 555 GCCGGC G^CCGGC
781 GCCGGC
PflMI 594 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
944 CCACTCACTGG
PvuII 133 CAGCTG CAG^CTG
831 CAGCTG
SfcI 425 CTGTAG C^T[GA][CT]AG
828 CTGCAG
SfoI 608 GGCGCC GGC^GCC
846 GGCGCC
SmaI 359 CCCGGG CCC^GGG
1037 CCCGGG
Van91I 594 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
944 CCACTCACTGG
XmaCI 359 CCCGGG C^CCGGG
1037 CCCGGG
XmaI 359 CCCGGG C^CCGGG
1037 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI TseI TspRI